A5051593516- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Eicosanoids and Hypertension Pharmacology
- Antioxidant Activity and Oxidative Stress
- Sulfur Compounds in Biology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Redox biology and oxidative stress
- Metabolism, Diabetes, and Cancer
- Stress Responses and Cortisol
- Cardiovascular and exercise physiology
- ATP Synthase and ATPases Research
- Exercise and Physiological Responses
- Genomics, phytochemicals, and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Heart Rate Variability and Autonomic Control
- Neurobiology and Insect Physiology Research
- Autophagy in Disease and Therapy
- Physical Activity and Health
- Thermoregulation and physiological responses
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Dietary Effects on Health
- Fibromyalgia and Chronic Fatigue Syndrome Research
University of Alabama at Birmingham
2015-2025
University of Pennsylvania
2016
Queen's University
2016
Louisiana State University Health Sciences Center Shreveport
2016
Boston University
2016
University of Wisconsin–Madison
2001
The M1 and M2 polarized phenotypes dictate distinctive roles for macrophages as they participate in inflammatory disorders. There has been growing interest the role of cellular metabolism macrophage polarization. However, it is currently unclear whether different aspects a specific metabolic program coordinately regulate this process. In study, we found that pyruvate dehydrogenase kinase 1 (PDK1), key regulatory enzyme glucose metabolism, plays an important differential activation...
To validate a Cardiometabolic Disease Staging (CMDS) system for assigning risk level diabetes, and all-cause cardiovascular disease (CVD) mortality.Two large national cohorts, CARDIA NHANES III, were used to CMDS. CMDS: Stage 0: metabolically healthy; 1: one or two metabolic syndrome factors [other than impaired fasting glucose (IFG)]; 2: IFG tolerance (IGT) (without IFG); 3: of three (IFG, IGT, and/or syndrome); 4: type 2 diabetes mellitus/CVD.In the study, compared with 0 healthy subjects,...
Temozolomide (TMZ) is an alkylating agent used for treating gliomas. Chemoresistance a severe limitation to TMZ therapy; there critical need understand the underlying mechanisms that determine tumor response TMZ. We recently reported chemoresistance related remodeling of entire electron transport chain, with significant increases in activity complexes II/III and cytochrome c oxidase (CcO). Moreover, pharmacologic genetic manipulation CcO reverses chemoresistance. Therefore, test hypothesis...
Glycated hemoglobin (HbA1c) has been advocated for the diagnosis of diabetes and prediabetes. Its performance commonly assessed in corroboration with elevated fasting plasma glucose (FPG), but not combination FPG 2-hr values. This study assesses receiver operating characteristics (ROC) curves HbA1c pertaining to diagnoses prediabetes by and/or glucose, effects age, gender, race.We utility diagnosing among 5395 adults without known from National Health Nutrition Examination Survey (NHANES)...
We conducted, in persons with Parkinson's disease (PD), a thorough assessment of neuromotor function and performance conjunction phenotypic analyses skeletal muscle tissue, further tested the adaptability PD to high-intensity exercise training. Fifteen participants (Hoehn Yahr stage 2-3) completed 16 wk training designed simultaneously challenge strength, power, endurance, balance, mobility function. Skeletal adaptations (P < 0.05) included myofiber hypertrophy (type I: +14%, type II: +36%),...
Abstract Hydrogen sulfide (H 2 S) is involved in numerous pathophysiological processes and shares overlapping functions with CO • NO. However, the importance of host-derived H S microbial pathogenesis unknown. Here we show that Mtb -infected mice deficient S-producing enzyme cystathionine β-synthase (CBS) survive longer reduced organ burden, pharmacological inhibition CBS reduces bacillary load mice. High-resolution respirometry, transcriptomics mass spectrometry establish stimulates...
The lipid products derived from the cyclooxygenase pathway can have diverse and often contrasting effects on vascular cell function. Cyclopentenone prostaglandins (cyPGs), such as 15-deoxy-Δ 12,14 -prostaglandin-J 2 (15d-PGJ ), a peroxisome proliferator–activated receptor-γ (PPARγ) agonist, been reported to cause endothelial apoptosis, yet in other types, cyPGs induce cytoprotective mediators, heat shock proteins, heme oxygenase-1, glutathione (GSH). Herein, we show human cells that low...
Exposure of cells to complex mixtures oxidized lipids such as those found in low-density lipoprotein (oxLDL) induce reactive oxygen and nitrogen species (ROS/RNS) formation. The source the ROS/RNS within is unknown; it thought they may be involved redox cell signaling. Although this possibility was initially overlooked, becoming clear that mitochondria, which are a superoxide hydrogen peroxide, play critical role response on exposure lipids. In study, we tested mitochondria potential...
Nitric oxide protects cells from oxidative stress through a number of direct scavenging reactions with free radicals but the effects nitric on regulation antioxidant enzymes are only now emerging. Using bovine aortic endothelial as model, we show that oxide, at physiological rates production (1-3 nM/s), is capable inducing synthesis glutathione mechanism involving gamma-glutamylcysteine synthetase and gamma-glutamyl transpeptidase. This novel signalling pathway cGMP-independent hypothesize...
Dysfunctional bioenergetics has emerged as a key feature in many chronic pathologies such diabetes and cardiovascular disease. This led to the mitochondrial paradigm which it been proposed that mtDNA sequence variation contributes disease susceptibility. In present study we show novel animal model of polymorphisms, MNX (mitochondrial-nuclear exchange) mouse, from C3H/HeN mouse inserted on C57/BL6 nuclear background vice versa test this concept. Our data major contribution susceptibility...
Osteocalcin is secreted by osteoblasts and improves insulin sensitivity in vivo, although mechanisms remain unclear. We tested the hypothesis that osteocalcin directly modulates cell biology insulin-targeted peripheral tissues. In L-6 myocytes, stimulated glucose transport both absence (basal) presence of insulin. Similarly, primary cultured adipocytes, carboxylated uncarboxylated increased basal insulin-stimulated as well sensitivity. also oxidation, though there was no effect on fatty acid...
Tribbles homolog 3 (TRIB3) was found to inhibit insulin-stimulated Akt phosphorylation and modulate gluconeogenesis in rodent liver. Currently, we examined a role for TRIB3 skeletal muscle insulin resistance. Ten insulin-sensitive, ten insulin-resistant, untreated type 2 diabetic (T2DM) patients were metabolically characterized by hyperinsulinemic euglycemic glucose clamps, biopsies of vastus lateralis obtained. Skeletal samples also collected from models including streptozotocin...
The relationships among skeletal muscle lipid peroxidation, intramyocellular content (IMCL), and insulin sensitivity were evaluated in nine insulin-sensitive (IS), 13 insulin-resistant (IR), 10 adults with type 2 diabetes (T2DM).Insulin was assessed by hyperinsulinemic-euglycemic clamp [glucose disposal rate (GDR)]. Lipid peroxidation 4-hydroxynonenal (HNE)-protein adducts general oxidative stress protein carbonyl content. All patients sedentary.Protein-HNE elevated 1.6-fold T2DM compared IS...
The enzyme cytochrome c oxidase (CcO) or complex IV (EC 1.9.3.1) is a large transmembrane protein that serves as the last in respiratory electron transport chain of eukaryotic mitochondria. CcO promotes switch from glycolytic to oxidative phosphorylation (OXPHOS) metabolism and has been associated with increased self-renewal characteristics gliomas. Increased activity tumors tumor progression after chemotherapy failure, patients primary glioblastoma multiforme high have worse clinical...
Exposure of endothelial cells to oxidized low-density lipoprotein (oxLDL) leads diverse cellular effects, including induction the intracellular antioxidant GSH. It is not known whether lipid-or protein-derived oxidation products cause GSH and this involves increased activity key enzyme in its synthesis, glutamate—cysteine ligase (GCL). Furthermore, effect oxLDL exposure on cell's ability combat oxidative stress has been previously examined. In present study we found that, bovine aortic...