A5065795242- Renal and related cancers
- Immune Cell Function and Interaction
- Genetic and Kidney Cyst Diseases
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- S100 Proteins and Annexins
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Cancer Research and Treatments
- Nonmelanoma Skin Cancer Studies
- Neonatal Respiratory Health Research
- Cancer-related gene regulation
- Virus-based gene therapy research
- PI3K/AKT/mTOR signaling in cancer
- Polyamine Metabolism and Applications
- Organ Donation and Transplantation
- Autopsy Techniques and Outcomes
- Ear and Head Tumors
- Protein Kinase Regulation and GTPase Signaling
- Sarcoidosis and Beryllium Toxicity Research
- Toxin Mechanisms and Immunotoxins
- Complement system in diseases
- Ureteral procedures and complications
University of Lucerne
2016-2024
Luzerner Kantonsspital
2017-2022
University Hospital of Zurich
2010-2014
University of Zurich
2005-2013
Institute of Cell Biology and Neurobiology
2009
University Hospital Innsbruck
2004
We investigated the proliferative capacity of renal proximal tubular cells in healthy rats. Previously, we observed that originate from differentiated cells. now found 1) by application bromo-deoxyuridine (BrdU) for 14 days and costaining BrdU, G(1)-phase marker cyclin D1 bulk S3 segment juvenile rats were involved proliferation; 2) although proliferation rate was about 10-fold higher compared with adult rats, roughly 40% G(1) both groups; 3) after a strong mitotic stimulus (lead acetate),...
Tubulo-interstitial fibrosis is a constant feature of chronic renal failure and it suspected to contribute importantly the deterioration function. In fibrotic kidney there exists, besides normal fibroblasts, large population myofibroblasts, which are supposedly responsible for increased production intercellular matrix. It has been proposed that myofibroblasts in originate from transformation tubular cells via epithelial-mesenchymal transition (EMT) or infiltration by bone marrow-derived...
Regulatory T cells (Tregs) represent a major population in the control of immune homeostasis and autoimmunity. Here we show that Fos-like 2 (Fosl2), TCR-induced AP1 transcription factor, represses Treg development controls Mice overexpressing Fosl2 (Fosl2tg) indeed systemic inflammatory phenotype, with infiltrates multiple organs. This phenotype is absent Fosl2tg × Rag2−/− mice lacking B cells, induces cell-intrinsic reduction responsible for phenotype. can transfer inflammation, which...
Diagnosis of salivary gland neoplasms is often challenging due to their high morphological diversity and overlaps. Several recurrent molecular alterations have been described recently, which can serve as powerful diagnostic tools potential therapeutic targets (e.g. NTRK or RET fusions). However, current sequential testing be expensive time consuming. In order facilitate the diagnosis neoplasms, we designed an all-in-one RNA-based next generation sequencing panel suitable for detection...
The most difficult thyroid tumors to be diagnosed by cytology and histology are conventional follicular carcinomas (cFTCs) oncocytic (oFTCs). Several microRNAs (miRNAs) have been previously found consistently deregulated in papillary carcinomas; however, very limited information is available for cFTC oFTC. aim of this study was explore miRNA deregulation find candidate markers that can used diagnostically.Thirty-eight (21 cFTCs, 17 oFTCs) 10 normal tissue samples were studied expression 381...
To determine whether metastasis to brain is associated with altered expression patterns of integrins, we investigated the αvβ3, αvβ5, αvβ6 and αvβ8 integrins in primary malignancies metastases breast, lung renal carcinomas malignant melanoma. Inhibitors αv are currently clinical trials for glioblastoma. The role process from other human tumors unknown. Immunohistochemistry novel integrin subtype specific rabbit monoclonal antibodies was performed on tissue microarrays archival material...
We searched for evidence a contribution of stem cells in growth the proximal S3 segments healthy rats. According to cell model, are undifferentiated and slow cycling; bulk cycling transit amplifying, rapidly cells. show following. 1) By continuous application thymidine analog (ThA) 7 days, epithelial kidneys display high-cycling rate. 2) Slow-cycling cells, identified by lack ThA uptake during 14 days up death expression cycle protein Ki67 at death, have same degree differentiation as...
Smoking increases the risk of developing rheumatoid arthritis (RA) and worsens course disease. In current study we analysed whether smoking can affect gene expression directly in joints.Synovial fibroblasts were incubated with 5% cigarette smoke extract changes detected using whole genome microarrays verified real-time PCR. Synovial tissues obtained from non-smoking patients RA undergoing joint replacement surgery mice exposed to or ambient air a body exposure chamber for 3 weeks.Microarray...
We studied 38 patients with malaria tropica over a period of 5 days during antiparasitic therapy. Serum or plasma levels interleukin (IL) 1beta, IL-6, IL-10, tumour necrosis factor-alpha (TNF-alpha), the soluble vascular adhesion molecule (sVCAM) and intracellular (sICAM) were determined by enzyme-linked immunosorbent assay. Protein C antithrombin III activity analysed chromogenic tests protein S clotting test. Antithrombin III, was significantly lower in severe displayed highly significant...
The efficacy of mammalian target rapamycin (mTOR) inhibitors is currently tested in patients affected by autosomal dominant polycystic kidney disease. Treatment with mTOR has been associated numerous side effects. However, the renal-specific effect inhibitor treatment cessation disease unknown. Therefore, we compared pulse and continuous everolimus Han:SPRD rats. Four-week-old male heterozygous wild-type rats were administered or vehicle gavage feeding for 5 wk, followed 7 wk without...
The eukaryotic translation initiation factor eIF3a is one of the core subunits complex eIF3, responsible for ribosomal subunit joining and mRNA recruitment to ribosome. It known play an important role in general as well specific translational regulation various gene products, among which many influence tumour development, progression, therapeutically pathways DNA damage repair. Therefore, beyond its protein synthesis, emerging regulator pathogenesis therapy response and, therefore, a...
SUMMARY: Aim: Autosomal dominant polycystic kidney disease (ADPKD) is characterized by an imbalance between tubular epithelial cell proliferation and apoptosis. We have previously shown that the mammalian target of rapamycin (mTOR) signalling pathway aberrantly activated in cystic kidneys Han:SPRD rats with ADPKD. Because Akt kinase upstream regulator mTOR, we hypothesized activity could be enhanced rats. Methods: Reverse transcription‐polymerase chain reaction, western blot, enzyme‐linked...
Fibroblast specific protein 1 (FSP1)/S100A4 is a calcium binding which has been linked to epithelial-mesenchymal transition, tissue fibrosis, pulmonary vascular disease, metastatic tumour development, increased cell motility and invasiveness. This reported be also expressed in newly formed differentiated fibroblasts used various studies demonstrate transition (EMT). We aimed characterize S100A4 positive cells different human compartments, with the focus on fibroblasts/myofibroblast. found...
During a study on the mTor pathway in rat kidney we observed striking increase of phosphorylation S6 kinase mitosis. In cryostat sections perfusion-fixed tissue mitotic cells appeared as bright spots immunofluorescence using an antibody specific for site Thr421/Ser424. They were easily spotted overviews with objective 4× and 10×. Immunofluorescence was weak during interphase. prophase it increased both nucleus cytoplasm remained subsequent phases All which found tubules interstitium...
Regulation of cytotoxic effector molecule expression in human CTLs after viral or bacterial activation is poorly understood. By using autologous dendritic cells (DCs) to prime T lymphocytes, we found perforin only highly up-regulated virus- (HSV-1, vaccinia virus) but not intracellular bacteria- (Listeria innocua, Listeria monocytogenes, Mycobacterium tuberculosis, Chlamydophila pneumoniae) activated CTLs. In contrast, larger quantities IFN-gamma and TNF-alpha were produced...
Abstract Fibroblast specific protein 1 (FSP1/S100A4) is an intracellular calcium binding which has been linked to epithelial-mesenchymal transition, tissue fibrosis, pulmonary vascular disease, metastatic tumour development, increased cell motility and invasiveness. This reported be uniquely expressed in newly formed differentiated fibroblasts used various studies demonstrate transition (EMT). However, S100A4 immunoreactivity does not solely fit the distribution shape of fibroblasts;...
ABSTRACT We previously demonstrated that Homeobox (HOX) transcription factors are differentially expressed between joint locations and can accurately assign synovial fibroblasts (SFs) to their correct location. show here the expression of 5’HOXD HOXD10, HOXD11, HOXD13 in SFs strikingly overlaps with predilection sites for development rheumatoid arthritis (RA). Changes gene after silencing 5’HOXDs aligned joint-specific differences RA SFs. In particular, we identify as regulator or primary...
Abstract Cancer–testis antigens (CTAgs) play a major role in the immune response against cancer, but their biological functions germ and cancer cells is still unclear. MAGE‐C1 MAGE‐C2 are two CTAgs located at Xq27 region of chromosome X frequently expressed multiple myeloma. Chromosomal rearrangements often occur We therefore investigated whether numerical structural chromosomal aberrations correlate with protein expression primary myelomas. To this aim, we designed new fluorescence situ...