A5020972809- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Genomics and Rare Diseases
- Microtubule and mitosis dynamics
- Plant Genetic and Mutation Studies
- Medieval European Literature and History
- Plant tissue culture and regeneration
- Advanced Fluorescence Microscopy Techniques
- Epigenetics and DNA Methylation
- Theology and Canon Law Studies
- DNA Repair Mechanisms
- Classical Studies and Legal History
- Chromosomal and Genetic Variations
- Neurogenetic and Muscular Disorders Research
- Cellular transport and secretion
MRC Laboratory for Molecular Cell Biology
2015-2020
University College London
2015-2020
MRC Laboratory of Molecular Biology
2014-2019
Hôpital Necker-Enfants Malades
2017
Sorbonne Université
2017
Norwich Research Park
2007
John Innes Centre
2007
Signaling from chemoattractant receptors activates the cytoskeleton of crawling cells for chemotaxis. We show using phosphoproteomics that different chemoattractants cause phosphorylation same core set around 80 proteins in Dictyostelium cells. Strikingly, majority these are phosphorylated at an [S/T]PR motif by atypical MAP kinase ErkB. Unlike most chemotactic responses, ErkB phosphorylations persistent and do not adapt to sustained stimulation with chemoattractant. integrates dynamic...
Dedifferentiation is a critical response to tissue damage, yet not well understood, even at basic phenomenological level. Developing Dictyostelium cells undergo highly efficient dedifferentiation, completed by most within 24 hr. We use this rapid investigate the control features of combining single cell imaging with high temporal resolution transcriptomics. Gene expression during dedifferentiation was predominantly simple reversal developmental changes, changes following pattern primarily...
In neutrophils and Dictyostelium, chemoattractant gradients generate directed cell migration by eliciting signaling events that bias intrinsic motility favor the production retention of upgradient pseudopods [1Stephens L. Milne Hawkins P. Moving towards a better understanding chemotaxis.Curr. Biol. 2008; 18: R485-R494Abstract Full Text PDF PubMed Scopus (136) Google Scholar, 2Andrew N. Insall R.H. Chemotaxis in shallow is mediated independently PtdIns 3-kinase biased choices between random...
ABSTRACT Embryonic development involves extensive and often rapid cell proliferation. An unavoidable side effect of proliferation is DNA damage. The consequences spontaneous damage during are not clear. Here, we define an approach to determine the effects on fate choice. Using single transcriptomics, identified a subpopulation Dictyostelium cells experiencing Damaged displayed high expression rad51, with gene induced by multiple types genotoxic stress. live imaging, tracked Rad51 from...
Summary For many crops, a narrowing genetic base is becoming an increasingly significant problem for improvements made through breeding. Commonly used breeding procedures systematically reduce diversity within elite gene pools. Here we describe new technique activation of genes in lines carrying Spm or dSpm transposon insertions. Activation Arabidopsis harbouring insertions their promoters can be induced by over‐expression the TnpA–VP16 fusion protein, which binds ends and activates local...