A5018004018- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- RNA Research and Splicing
- RNA regulation and disease
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Polyamine Metabolism and Applications
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
- interferon and immune responses
- DNA Repair Mechanisms
- Inflammatory Myopathies and Dermatomyositis
- Glycosylation and Glycoproteins Research
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- CAR-T cell therapy research
- Biochemical and Molecular Research
Rutgers, The State University of New Jersey
2014-2024
Rutgers New Jersey Medical School
2012-2024
The University of Texas Rio Grande Valley
2024
Case Western Reserve University
2023
The Ohio State University
2022
Eli Lilly (United States)
2017
Newark Academy
2011
University Hospital, Newark
2010
In-Q-Tel
2008
University of Colorado Colorado Springs
2007
P-TEFb is a key regulator of the process controlling processivity RNA polymerase II and possesses kinase activity that can phosphorylate carboxy-terminal domain largest subunit II. Here we report cloning small Drosophila finding it encodes Cdc2-related protein kinase. Sequence comparison suggests with 72% identity, PITALRE, could be human homolog protein. Functional homology was suggested by transcriptional analysis an promoter HeLa nuclear extract depleted PITALRE. Because lost ability to...
An autoantibody known as anti-Jo-1 antibody is found in 25% of patients with myositis. Its prevalence both myositis and cryptogenic fibrosing alveolitis was 68% (13 out 19 patients), compared 7.5% alone (four 53) 3% (two 62). Anti-Jo-1 may be useful indicating alveolitis. Raynaud9s phenomenon, the sicca syndrome, mild arthritis are also often part syndrome.
The interferon-induced protein kinase DAI, the double-stranded RNA (dsRNA)-activated inhibitor of translation, plays a key role in regulating synthesis higher cells. Once activated, process that involves autophosphorylation, it phosphorylates initiation factor eIF-2, leading to inhibition polypeptide chain initiation. activity DAI is controlled by regulators, including dsRNA activators and highly structured single-stranded RNAs which block activation dsRNA. To elucidate mechanism activation,...
The proliferating cell nuclear antigen (PCNA), also known as cyclin and DNA polymerase delta auxiliary factor, is present in reduced amounts nongrowing cells synthesized at a greater rate the S phase of growing cells. recently discovered involvement PCNA replication suggested that this pattern expression functions to regulate synthesis. We have investigated possibility further by examining synthesis, stability, accumulation HeLa fractionated centrifugal elutriation into nearly synchronous...
A cell‐free system from mouse Krebs II ascites cells is described which responds by increased amino acid incorporation into protein on addition of encephalomyocarditis virus (EMC‐RNA). RNA other sources does not produce this response. The stimulation EMC‐RNA occurs over a narrow range Mg 2+ concentrations and maximal at 5 mM, optimal for the endogenous lower than that required in presence poly(U). EMC‐RNA‐directed product distinguished products its size composition. After an initial lag 4...
The human double-stranded RNA-dependent protein kinase (PKR) is an important component of the interferon response to virus infection. activation PKR accompanied by autophosphorylation at multiple sites, including one in N-terminal regulatory region (Thr-258) that required for full activity. Several kinases are activated phosphorylation between subdomains VII and VIII, referred as loop. We show Thr-446 Thr-451 loop vivo vitro high-level Mutation either residue Ala impaired translational...
Myotonic dystrophy is caused by an expanded CTG repeat in the 3′ untranslated region of DM protein kinase (DMPK) gene. The triggers nuclear retention mutant DMPK transcripts, but resulting underexpression probably does not fully account for severe phenotype. One proposed disease mechanism that accumulation CUG repeats may interfere with function. Here we show thermal melting and nuclease digestion studies form highly stable hairpins. Furthermore, bind to dsRNA-binding domain PKR,...