A5032542388- Genetics, Aging, and Longevity in Model Organisms
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Fungal and yeast genetics research
- Circadian rhythm and melatonin
- Dietary Effects on Health
- RNA Research and Splicing
- Microbial Metabolic Engineering and Bioproduction
- Epigenetics and DNA Methylation
- ATP Synthase and ATPases Research
- Biochemical Acid Research Studies
- FOXO transcription factor regulation
- Gene Regulatory Network Analysis
- Histone Deacetylase Inhibitors Research
- Health, Environment, Cognitive Aging
- DNA Repair Mechanisms
- Metabolism and Genetic Disorders
- Spaceflight effects on biology
- Nuclear Structure and Function
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Bioinformatics and Genomic Networks
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
Amsterdam University Medical Centers
2018-2025
University of Amsterdam
2018-2025
Amsterdam UMC Location University of Amsterdam
2018-2025
Integrated Cardio Metabolic Centre
2018-2023
Karolinska Institutet
2018-2023
University of Ottawa
2022
University Medical Center Groningen
2015-2019
University of Groningen
2013-2019
University of Liverpool
2013-2014
Genomics (United Kingdom)
2013-2014
An integrated account of the molecular changes occurring during process cellular aging is crucial towards understanding underlying mechanisms. Here, using novel culturing and computational methods as well latest analytical techniques, we mapped proteome transcriptome replicative lifespan budding yeast. With age, found primarily proteins involved in protein biogenesis to increase relative their transcript levels. Exploiting dynamic nature our data, reconstructed high-level directional...
Are human ovarian aging and the age-related female fertility decline caused by oxidative stress mitochondrial dysfunction in oocytes?We found damage oocytes of advanced maternal age, even at primordial follicle stage, confirmed such oocytes, which likely resulted use alternative energy sources.Signs reactive oxygen species-induced have been observed maturing follicles, early stages embryogenesis. However, although recent evidence indicates that also follicles metabolically active...
The ability to perceive and respond harmful conditions is crucial for the survival of any organism. transcription factor DAF-16/FOXO central these responses, relaying distress signals into expression stress resistance longevity promoting genes. However, its sufficiency in fulfilling this complex task has remained unclear. Using C. elegans, we show that DAF-16 does not function alone but as part a transcriptional regulatory module, together with HLH-30/TFEB. Under conditions, both factors...
Slowing down translation in either the cytosol or mitochondria is a conserved longevity mechanism. Here, we found non-interventional natural correlation of mitochondrial and cytosolic ribosomal proteins (RPs) mouse population genetics, suggesting translational balance. Inhibiting C. elegans through mrps-5 RNAi repressed translation. Transcriptomics integrated with proteomics revealed that this inhibition specifically reduced efficiency mRNAs required growth pathways while increasing stress...
Multiple studies characterizing the human ageing phenotype have been conducted for decades. However, there is no centralized resource in which data on multiple age-related changes are collated. Currently, researchers must consult several sources, including primary publications, order to obtain at various levels. To address this and facilitate integrative, system-level of we developed Digital Ageing Atlas (DAA). The DAA a one-stop collection covering different biological levels (molecular,...
Aging strongly influences human morbidity and mortality. Thus, aging-preventive compounds could greatly improve our health lifespan. Here we screened for such compounds, known as geroprotectors, employing the power of transcriptomics to predict biological age. Using age-stratified tissue transcriptomes machine learning, generated age classifiers applied these transcriptomic changes induced by 1,309 different in cells, ranking their ability induce a "youthful" transcriptional state. Testing...
Nuclear transport is facilitated by the Pore Complex (NPC) and essential for life in eukaryotes. The NPC a long-lived exceptionally large structure. We asked whether quality control compromised aging mitotic cells. Our images of single yeast cells during aging, show that abundance several components assembly factors decreases. Additionally, single-cell histories reveal better maintain those are longer lived. presence herniations at nuclear envelope aged suggests misassembled NPCs accumulated...
Over 80% of women with X-linked adrenoleukodystrophy (ALD) develop spinal cord disease in adulthood for which treatment is supportive only. For future clinical trials quantitative data on progression rates are essential. Moreover, diagnosis can be challenging ALD women, as the most important diagnostic biomarker normal 15–20%. Better biomarkers needed. The purpose this single centre cross-sectional follow-up study was to assess whether Expanded Disability Status Scale (EDSS), AMC Linear...
Impaired insulin/IGF-1 signaling (IIS) and caloric restriction (CR) prolong lifespan in the nematode C. elegans. However, a cross comparison of these longevity pathways using multi-omics integration approach is lacking. In this study, we aimed to identify key metabolite fingerprints that are shared between IIS CR worm models integration. We generated transcriptomics metabolomics data from long-lived strains, i.e. daf-2 (impaired IIS) eat-2 (CR model) compared them with wild-type strain N2....
The deregulation of metabolism is a hallmark aging. As such, changes in the expression metabolic genes and profiles amino acid levels are features associated with aging animals. We previously reported that most acids decline age Caenorhabditis elegans (C. elegans). Glycine, contrast, substantially accumulates C. elegans. In this study we show coupled to decrease gene enzymes important for glycine catabolism. further supplementation significantly prolongs lifespan, early adulthood its...
There is a large variability in lifespans of individuals even if they are genetically identical and raised under the same environmental conditions. Our recent system wide study replicative aging baker's yeast predicts that protein biogenesis driver aging. Here, we address how natural variation lifespan within wild-type populations cells correlates to three biogenesis-related parameters, namely cell size, ribosomal Rpl13A-GFP levels, division times. Imaging wild type microfluidic devices...
A comprehensive description of the phenotypic changes during cellular aging is key towards unraveling its causal forces. Previously, we mapped age-related in proteome and transcriptome (Janssens et al., 2015). Here, employing same experimental procedure model-based inference, generate a account metabolic replicative life Saccharomyces cerevisiae. With age, found decreasing metabolite levels, growth substrate uptake rates accompanied by switch from aerobic fermentation to respiration, with...
Mitochondrial form and function are closely interlinked in homeostasis aging. Inhibiting mitochondrial translation is known to increase lifespan C. elegans, accompanied by a fragmented network. However, whether this link between morphology causal longevity remains uncharacterized. Here, we show elegans that disrupting network blocking fission or fusion synergizes with reduced prolong stimulate stress response such as the unfolded protein response, UPRMT. Conversely, immobilizing through...
ABSTRACT Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand; for instance, research related to nutrition aging. The nematode Caenorhabditis elegans is a key model organism these fields, owing the large repository of available C. mutants their convenient natural lifespan. Here, we describe robust sensitive analytical method semi-quantitative analysis >100 polar (metabolomics) >1000 apolar (lipidomics) metabolites elegans, using...