A5075993246- Nuclear Physics and Applications
- Bone health and osteoporosis research
- Bone Tissue Engineering Materials
- Calcium Carbonate Crystallization and Inhibition
- Hydrocarbon exploration and reservoir analysis
- Bone and Dental Protein Studies
- Advanced X-ray Imaging Techniques
- High-pressure geophysics and materials
- Nuclear reactor physics and engineering
- Alkaline Phosphatase Research Studies
- NMR spectroscopy and applications
- Spectroscopy and Chemometric Analyses
- Dental materials and restorations
- Orthodontics and Dentofacial Orthopedics
- Analytical Chemistry and Chromatography
- Parathyroid Disorders and Treatments
- Boron Compounds in Chemistry
- Cephalopods and Marine Biology
- Paleontology and Evolutionary Biology
- X-ray Diffraction in Crystallography
- Paleontology and Stratigraphy of Fossils
- Bone health and treatments
- Chemical Thermodynamics and Molecular Structure
- Medical Imaging Techniques and Applications
- Crystallization and Solubility Studies
Aarhus University
2019-2025
Abstract Bone has a complex hierarchical structure with structural integration from nm to cm. The understanding of bone is developing rapidly due improvements in available methodologies that allow unravelling structures across several length scales. These methods include advances electron microscopy, particular, focused ion beam scanning microscopy (FIB‐SEM), confocal laser techniques, X‐ray imaging, diffraction tomography (XRD‐CT), and tensor (small angle scattering tomgraphy, SAXS‐TT wide...
The biomineralization of bone remains a puzzle. During Haversian remodeling in the dense human cortical bone, osteoclasts excavate tunnel that is then filled by osteoblasts with layers varying fibril orientations, resulting lamellar motif. Such represents an excellent possibility to increase our understanding as material well studying spatio/temporal variations biomineral across osteon. To this end, fluorescence computed tomography and diffraction scattering sub-micrometer resolution applied...
ABSTRACT PHEX is predominantly expressed by bone and tooth-forming cells, its inactivating mutations in X-linked hypophosphatemia (XLH) lead to renal phosphate wasting severe hypomineralization of bones teeth. Also present XLH are hallmark hypomineralized periosteocytic lesions (POLs, halos) that persist despite stable correction serum (Pi) improves bulk mineralization. In XLH, mineralization-inhibiting osteopontin (OPN, a substrate for PHEX) accumulates the extracellular matrix bone. To...
Accurate 3D characterization of osteocyte lacunae is important when investigating the role osteocytes under various physiological and pathological conditions but remains a challenge. With continued development laboratory X-ray micro-computed tomography, an increasing number studies employ these techniques beyond traditional bone morphometry to quantify lacunae. However, there lack knowledge on effect measurement parameters image quality resolution in turn quantification. Herein, we have...
The use of a phase-retrieval technique for propagation-based phase-contrast neutron imaging with polychromatic beam is demonstrated. This enables samples low absorption contrast and/or improving the signal-to-noise ratio to facilitate e.g. time-resolved measurements. A metal sample, designed be close phase pure object, and bone sample canals partially filled D2O were used demonstrating technique. These imaged followed by retrieval. For both ratios significantly improved and, in case...
Computed tomography spans a versatile set of techniques that range several length scales and modalities. It is common to them all the sample must be prepared in way which allows for addressing scientific question(s) posed as well being suitable specific setup experiment. We present two lathe-based preparation workflows developed prepare biomineralized samples (here bone) very different experiments terms setup, types questions asked, requirements. The first experiment, involving measurement...
Propagation-based phase contrast, for example in the form of edge enhancement is well established within X-ray imaging but not widely used neutron imaging. This technique can help increase contrast low-attenuation samples may confuse quantitative absorption measurements. Therefore, it important to understand experimental parameters that cause and amplify or dampen this effect order optimize future experiments properly. Two simulation approaches have been investigated, a wave-based...
Guanine crystals are frequently encountered in nature the β-polymorph to create structural colors, enhance vision of creatures, and for camouflage. Unfortunately, it is challenging control crystallization guanine aqueous conditions laboratory because its low solubility water. Here, we crystallize an environment under confinement. We employ water–oil–water double emulsions stabilized with a metal–ligand functionalized surfactant as selectively permeable containers by dynamically adjusting pH...
Liquid plays an important role in bone that has a complex 3D hierarchical pore structure. However, liquid (water) is difficult to discern from e.g. organic matrix by X-ray imaging. Therefore, we use correlative approach using both high resolution and neutron Human femoral with adsorbed into some of the pores was imaged Neutron Microscope at ICON beamline, SINQ PSI, lab-based μCT 2.7 μm voxel size. Segmentation two datasets showed that, even though clearly distinguishable data not data, it...
Stomatopods are ferocious hunters that use weaponized appendages to strike down their pray. The clubs of species such as Odontodactylus scyllarus undergo tremendous forces, and in consequence they have intricate structures, consisting hydroxyapatite, chitin, amorphous calcium phosphate carbonate, occasionally calcite. These materials distributed differently across the four major zones dactyl club: impact, periodic lateral medial, striated regions. While stomatopod structure been studied for...
Bone has a complex hierarchical structure, which is essential for its performance. typically replete with cells called osteocytes that are embedded in the mineralized bone matrix osteocyte lacunae, interconnected by canaliculi only few hundred nanometer wide to form vast cellular network. Our understanding of lacuno-canalicular network been limited because difficulties image within opaque 3D. Synchrotron X-ray computed tomography ideally suited study it combines high penetration power X rays...
We demonstrate the use of a phase retrieval technique for propagation-based contrast neutron imaging with polychromatic beam. This enables samples low absorption and/or improving signal-to-noise ratio to facilitate e.g. time resolved measurements. A metal sample, designed be close pure object, and bone sample canals partially filled D2O were used demonstrating technique. These imaged beam followed by retrieval. For both significantly improved in case allowed separation D2O, which is...