A5008502758- Cancer-related molecular mechanisms research
- Veterinary Oncology Research
- Sarcoma Diagnosis and Treatment
- Virus-based gene therapy research
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Molecular Biology Techniques and Applications
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- MicroRNA in disease regulation
- Infectious Diseases and Mycology
- Hedgehog Signaling Pathway Studies
- interferon and immune responses
- RNA Research and Splicing
- Vascular Tumors and Angiosarcomas
- Immune responses and vaccinations
- Viral Infections and Outbreaks Research
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Immune Response and Inflammation
University of Minnesota
2015-2024
University of Minnesota Medical Center
2013-2024
University of Minnesota System
2018
Masonic Cancer Center
2015
Breast Cancer Care
2015
This study examines the extent to which memory CD4+ T cells share immunosurveillance strategies with CD8+ resident (TRM). After acute viral infection, predominantly used residence survey nonlymphoid tissues, albeit not as stringently observed for cells. In contrast, were more likely be within lymphoid organs than Migration properties of memory-phenotype in non-SPF parabionts similar, generalizing these results diverse infections and conditions. TRM shared overlapping transcriptional...
Abstract Overall survival of patients with osteosarcoma (OS) has improved little in the past three decades, and better models for study are needed. OS is common large dog breeds genetically inducible mice, making disease ideal comparative genomic analyses across species. Understanding level conservation intertumor transcriptional variation species how it associated progression to metastasis will enable us more efficiently develop effective strategies manage improve therapy. In this study,...
Abstract Angiosarcoma is a highly aggressive cancer of blood vessel–forming cells with few effective treatment options and high patient mortality. It both rare heterogenous, making large, well-powered genomic studies nearly impossible. Dogs commonly suffer from similar cancer, called hemangiosarcoma, breeds like the golden retriever carrying heritable genetic factors that put them at risk. If clinical similarity canine hemangiosarcoma human angiosarcoma reflects shared etiology, dogs could...
Analyses of healthy tissue reveal signatures that identify resident memory CD8
We performed genomewide gene expression analysis of 35 samples representing 6 common histologic subtypes canine lymphoma and bioinformatics analyses to define their molecular characteristics. Three major groups were defined on the basis profiles: (1) low-grade T-cell lymphoma, composed entirely by T-zone lymphoma; (2) high-grade consisting lymphoblastic peripheral not otherwise specified; (3) B-cell marginal diffuse large Burkitt lymphoma. Interspecies comparative profiles also showed that...
Abstract Background Deregulation of microRNA (miRNA) transcript levels has been observed in many types tumors including osteosarcoma. Molecular pathways regulated by differentially expressed miRNAs may contribute to the heterogeneous tumor behaviors naturally occurring cancers. Thus, tumor-associated miRNA expression provide informative biomarkers for disease outcome and metastatic potential osteosarcoma patients. We showed previously that clusters at 14q32 locus are downregulated human...
Genital immunology is a key determinant of human immunodeficiency virus (HIV) susceptibility. Both factors are modulated by bacterial vaginosis (BV) and, to some extent, Lactobacillus iners, the genital spp. that predominates in African, Caribbean, and other Black (ACB) women. We conducted clinical trial assess impact oral metronidazole treatment on immune parameters HIV acquisition risks Kenyan women with BV. The primary endpoint was ex vivo cervical CD4+ T-cell susceptibility after 1...
Abstract Background The etiology of hemangiosarcoma remains incompletely understood. Its common occurrence in dogs suggests predisposing factors favor its development this species. These could represent a constellation heritable characteristics that promote transformation events and/or facilitate the establishment microenvironment is conducive for survival malignant blood vessel-forming cells. hypothesis study was characteristic molecular features distinguish from non-malignant endothelial...
The oral mucosa is a frontline for microbial exposure and juxtaposes several unique tissues mechanical structures. Based on parabiotic surgery of mice receiving systemic viral infections or co-housing with microbially diverse pet shop mice, we report that the harbors CD8+ CD103+ resident memory T cells (TRM), which locally survey without recirculating. Oral antigen re-encounter during effector phase immune responses potentiated TRM establishment within tongue, gums, palate, cheek. Upon...
Fas ligand (FasL) gene therapy for cancer has shown promise in rodents; however, its efficacy higher mammals remains unknown. Here, we used intratumoral FasL delivered an adenovirus vector (Ad-FasL) as neoadjuvant to standard of care 56 dogs with osteosarcoma. Tumors from treated had greater inflammation, necrosis, apoptosis, and fibrosis at day 10 (amputation) compared pretreatment biopsies or tumors that did not receive Ad-FasL. Survival improvement was apparent inflammation...
Humans experience frequent respiratory infections. Immunology and vaccinology studies in mice are typically performed naive specific pathogen-free animals responding to their very first challenge. We found that the infection induces lifelong enlargement of lung-draining mediastinal lymph nodes (medLNs). Furthermore, infection-experienced medLNs supported better T cell surveillance effector responses new unrelated infections exhibited more biased accumulation memory establishment within lung....
Osteosarcoma (OS) is a heterogeneous and rare disease with disproportionate impact, as it mainly affects children adolescents. Lamentably, more than half of patients OS succumb to metastatic disease. Clarification the etiology disease, development better strategies manage progression, methods guide personalized treatments are among unmet health needs for patients. Progress in managing has been hindered by extreme heterogeneity OS; thus, models that accurately recapitulate natural needed. For...
Rapidly growing tumor cells require a nutrient-rich environment in order to thrive, therefore, restricting access certain key amino acids, such as arginine, often results the death of malignant cells, which frequently display defective cell cycle check-point control. Healthy by contrast, become quiescent and remain viable under arginine restriction, displaying full recovery upon return arginine-rich conditions. The use arginase therapy restrict available for selectively targeting is...
Abstract Central memory T (TCM) cells patrol lymph nodes and perform conventional responses upon re-stimulation: proliferation, migration, differentiation into diverse cell subsets while also self-renewing. Resident (TRM) are parked within single organs, share properties with terminal effectors, contribute to rapid host protection. We observed that reactivated TRM rejoined the circulating pool. Epigenetic analyses revealed align closely populations, bearing little resemblance recently...