A5103045573- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- GDF15 and Related Biomarkers
- Nuclear Structure and Function
- Acute Ischemic Stroke Management
- Cancer-related molecular mechanisms research
- Dental Trauma and Treatments
- Head and Neck Anomalies
- Oral and Maxillofacial Pathology
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Vitamin K Research Studies
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Vitamin C and Antioxidants Research
- RNA regulation and disease
- Barrier Structure and Function Studies
- Metabolism and Genetic Disorders
- Vitamin D Research Studies
- dental development and anomalies
- Tracheal and airway disorders
- Neurological Disease Mechanisms and Treatments
- Genomics and Rare Diseases
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2024
Universidade Federal de Uberlândia
2024
Helmholtz Zentrum München
2022-2023
Leipzig University
2023
University Hospital Leipzig
2022
Universidade Estadual de Campinas (UNICAMP)
2019-2022
Aarhus University
2022
Brazilian Institute of Neuroscience and Neurotechnology
2020
Centro Universitário do Triângulo
2017
Obesity promotes diverse pathologies, including atherosclerosis and dementia, which frequently involve vascular defects endothelial cell (EC) dysfunction. Each organ has distinct EC subtypes, but whether ECs are differentially affected by obesity is unknown. Here we use single-cell RNA sequencing to analyze transcriptomes of ~375,000 from seven organs in male mice at progressive stages identify organ-specific vulnerabilities. We find that deregulates gene expression networks, lipid handling,...
Lipodystrophy syndromes (LDs) are characterized by loss of adipose tissue, metabolic complications such as dyslipidemia, insulin resistance, and fatty liver disease, well accelerated atherosclerosis. As a result tissue deficiency, the systemic concentration adipokine leptin is reduced. A current promising therapeutic option for patients with LD treatment recombinant (metreleptin), resulting in reduced risk mortality. Here, we investigate effects on endothelial to mesenchymal transition...
White adipose tissue (WAT) is essential for energy storage and metabolic homeostasis, plays a central role in the pathology of obesity type 2 diabetes. Vascular networks within WAT support oxygen nutrient supply regulate adipogenesis, yet mechanisms underlying endothelial-adipocyte crosstalk under both healthy dysfunctional conditions remain unclear. Here, we present vascularized organoid (vAO) model engineered from human adipose-derived stem cells induced pluripotent cell-derived...
Pericytes (PCs) are essential components of the blood brain barrier. Brain PCs critical for dynamically regulating flow, maintaining vascular integrity and their dysregulation is associated with a myriad disorders such as Alzheimer's disease. To understand physiological molecular functions, studies have increasingly focused on primary PC isolation culture. Multiple methods culture been developed over years, however, it still unclear how compare to in vivo counterparts. address this question,...
INTRODUÇÃO/JUSTIFICATIVA: As doenças raras são um conjunto diverso de condições patológicas geralmente crônicas, baixa frequência na população em geral, mas, como grupo, tornam-se expressivas números pessoas afetadas. Considera-se aquelas que afetam até 65 cada 100.000 indivíduos, ou seja, 1,3 para 2.000 indivíduos. O número total é difícil determinação, mas estima-se haja mais 6.000 e 70,0% crianças. Elas caracterizadas por uma ampla diversidade sinais sintomas as manifestações podem...
Abstract Alzheimer’s Disease (AD) is characterized by progressive cognitive decline and synaptic dysfunction, often associated with amyloid-beta accumulation microglial alterations. Here, we investigate the role of Sodium-dependent Vitamin C Transporter 2 (SVCT2) in microglia to modulate AD-like pathology mice. Using a combination RNA sequencing, advanced quantitative proteomics, electrophysiology, behavioral tests, high-throughput imaging, viral gene delivery, explore interplay between...
Ischemic stroke (IS) is one of the most common causes mortality worldwide and represents 80% all cases. Previous studies have shown that some metabolites can be markers oxidative injury to vascular endothelial cells neuromodulation after brain ischemia. Biomarkers are small molecules dysregulated during diseases used improve diagnosis determine prognosis, including responses treatment. This study aims identify if there specific in plasma patients with IS acute chronic stages. Currently, we...
Abstract Objectives : to investigate exosome-derived transfer RNAs (tRNAs) in the plasma of patients chronic phase ischemic stroke (IS). tRFs may be disease biomarkers, especially for disorders linked hypoxic and extracellular stress. Material methods We assessed samples from ten who had (IS), collected them six months after ictus, compared obtained controls. used small RNA sequencing tRNA identification quantification. Results found that most abundant class identified patients’ were 3′...
Abstract Introduction and purpose Lipodystrophy (LD) syndromes are characterized by the loss of adipose tissue resulting in metabolic complications accelerated atherosclerosis. The systemic concentration adipokine leptin is reduced LD as a result deficiency. A therapeutical option to treat substitution leptin, which improves reduces mortality. However, vascular effects remain largely unknown. Here we analyze direct on system development Methods results Treatment human endothelial cells (ECs)...