A5053962486- RNA Research and Splicing
- Heat shock proteins research
- Cell death mechanisms and regulation
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Autophagy in Disease and Therapy
- Melanoma and MAPK Pathways
- Kruppel-like factors research
- Mitochondrial Function and Pathology
Universitat Pompeu Fabra
2020-2022
Institute for Research in Biomedicine
2020-2022
Goethe University Frankfurt
2016-2020
Protein biogenesis is tightly linked to protein quality control (PQC). The role of PQC machinery in recognizing faulty polypeptides becoming increasingly understood. Molecular chaperones and cytosolic vacuolar degradation systems collaborate detect, repair, or hydrolyze mutant, damaged, mislocalized proteins. On the other hand, contribution cofactor binding-related enzyme maturation remains largely unexplored, although loading a represents an all-or-nothing transition regard enzymatic...
Abstract Post-transcriptional methylation of N6-adenine and N1-adenine can affect transcriptome turnover translation. Furthermore, the regulatory function N6-methyladenine (m6A) during heat shock has been uncovered, including enhancement phase separation potential RNAs. In response to acute stress, e.g. shock, orderly sequestration mRNAs in stress granules (SGs) is considered important protect transcripts from irreversible aggregation. Until recently, role N1-methyladenine (m1A) on remains...
Abstract Tumor cells adapt their metabolism to meet the energetic and anabolic requirements of high proliferation invasiveness. The metabolic addiction has motivated development therapies directed at individual biochemical nodes. However, currently there are few possibilities target multiple enzymes in tumors simultaneously. Flavin-containing enzymes, ca. 100 proteins humans, execute key biotransformations mammalian cells. To expose addiction, we inactivated a substantial fraction...
Stress-activated protein kinases (SAPKs) enhance survival in response to environmental changes. In yeast, the Hog1 SAPK and Mrc1, a required for DNA replication, define safeguard mechanism that allows eukaryotic cells prevent genomic instability upon stress during S-phase. Here we show that, mammals, p38 Claspin—the functional homolog of Mrc1—protect from damage osmostress We demonstrate phosphorylates Claspin either mutation p38-phosphorylation sites or inhibition suppresses protective role...
Cells respond to protein misfolding and aggregation in the cytosol by adjusting gene transcription a number of post-transcriptional processes. In parallel functional reactions, cellular structure changes as well; however, mechanisms underlying early adaptation compartments cytosolic are less clear. Here we show that mammalian ubiquitin ligase C-terminal Hsp70-interacting (CHIP), if freed from chaperones during acute stress, can dock on membranes thus performing proteostasis sensor function....
At any stage of their lifecycle, mRNAs are coated by specialized proteins. One few circumstances when free mRNA appears in the cytosol is disassembly polysomes during stress-induced shutdown protein synthesis. Using quantitative mass spectrometry, we sought to identify RNA-interacting cellular machinery heat-shocked mammalian cells. Free RNA-associated proteins displayed higher disorder and larger size, which supports role multivalent interactions initial phase association with RNAs stress....
Abstract Post-transcriptional modifications of nucleotide bases can effect several aspects mRNA function. For example, the recent work has established role m 6 A in coordinated regulation transcriptome turnover and translation during cellular differentiation tumorigenesis. The levels 1 mRNAs reach up to 10% those A, yet functional consequences this modification are much less clear. Here we show that N1-methyladenine protects against aberrant interactions heat shock amyloidogenesis mammalian...
ABSTRACT Transient sequestration of proteins and RNA is an essential principle cellular reaction to stress. Compared polypeptides, less known about the role released from polysomes during acute proteostasis Using quantitative mass spectrometry, we identified a set assembled by free in heat-shocked mammalian cytosol. RNA-associated displayed higher disorder larger size, which supports multivalent interactions initial phase granule formation. Structural features interactors defined them as...