A5016417647- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Clostridium difficile and Clostridium perfringens research
- Metabolism and Genetic Disorders
- Nanopore and Nanochannel Transport Studies
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Erythrocyte Function and Pathophysiology
- Bacterial Infections and Vaccines
- Ion-surface interactions and analysis
- Metabolomics and Mass Spectrometry Studies
- Electrostatics and Colloid Interactions
- Blood disorders and treatments
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Multiple Sclerosis Research Studies
- Insect symbiosis and bacterial influences
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- RNA modifications and cancer
- Neonatal Health and Biochemistry
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Burkholderia infections and melioidosis
University of Exeter
2012-2024
MRC Mitochondrial Biology Unit
2006-2008
Wellcome Trust
2008
MRC Human Nutrition Research
2006
Babraham Bioscience Technologies (United Kingdom)
2006
Mechanisms of mitochondrial DNA (mtDNA) maintenance have recently gained wide interest owing to their role in inherited diseases as well aging. Twinkle is a new 5′–3′ helicase, defects which we previously shown underlie disease, progressive external ophthalmoplegia with multiple mtDNA deletions. Mouse highly similar the human counterpart, suggesting conserved function. Here, characterized mouse gene and expression profile report that patterns are not between mouse, but synchronized adjacent...
NetB is a pore-forming toxin produced by Clostridium perfringens and has been reported to play major role in the pathogenesis of avian necrotic enteritis, disease that emerged due removal antibiotics animal feedstuffs. Here we present crystal structure pore form solved 3.9 Å. The heptameric assembly shares structural homology staphylococcal α-hemolysin. However, rim domain, region thought interact with target cell membrane, shows sequence divergence leading alteration phosphocholine binding...
Background: It was recently reported that, using Western blotting, some multiple sclerosis (MS) patients in the United States had antibodies against epsilon toxin (Etx) from Clostridium perfringens, suggesting that may play a role disease. Objective: We investigated for serum Etx UK with clinically definite (CDMS) or presenting isolated syndrome (CIS) optic neuritis (ON) and age- gender-matched controls. Methods: tested sera CDMS, CIS ON controls by blotting. also CDMS reactivity linear...
Artificial transfer of mitochondrial genes to the nucleus has implications for understanding function, evolution, and human health. Therefore, we created nuclear compatible versions subunit a (A6) ATP synthase, linked targeting signal. Expression were compared Chlamydomonas reinhardtii, which naturally occurs in nucleus. Algal was targeted mitochondria more efficiently than variants. However, there no evidence improved function cultured cells; on contrary, long-term expression algal...
Maintenance of an intact mitochondrial genome is essential for oxidative phosphorylation in all eukaryotes. Depletion copy number can have severe pathological consequences due to loss respiratory capacity. In Saccharomycescerevisiae, several bifunctional metabolic enzymes been shown be required DNA (mtDNA) maintenance. For example, Ilv5 branched chain amino acid biosynthesis and mtDNA stability. We identified OXA1 TIM17 as novel multicopy suppressors instability ilv5 cells. addition,...
Necrotic enteritis toxin B (NetB) is a β-pore-forming produced by Clostridium perfringens and has been identified as key virulence factor in the pathogenesis of avian necrotic enteritis, disease causing significant economic damage to poultry industry worldwide. In this study, site-directed mutagenesis was used identify amino acids that play role NetB oligomerisation pore-formation. K41H showed significantly reduced toxicity towards LMH cells human red blood relative wild type toxin. unable...
Clostridium perfringens epsilon toxin (Etx) is a pore forming that causes enterotoxaemia in ruminants and may be cause of multiple sclerosis humans. To date, most vitro studies Etx have used the Madin-Darby canine kidney (MDCK) cell line. However, using Chinese hamster ovary (CHO) cells engineered to express putative receptor, myelin lymphocyte protein (MAL), suggest amino acids important for activity differ between species. In this study, we investigated role Y42, Y43 H162, previously...
Abstract Aerolysin-like proteins are a family of β-pore-forming toxins which widely present in all kingdoms life. Recently, this is gaining attention because their biotechnological application as nanopore sensors for sensing and sequencing biomolecules. Here, we explore the possibilities using knowledge sequence structure to screen new potential candidates. However, spite conserved structural fold, identity very low. This complicates alignment, hindering understanding pore properties,...