A5041850751- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- Cardiac Arrhythmias and Treatments
- Cardiac electrophysiology and arrhythmias
- RNA Research and Splicing
- Cardiac pacing and defibrillation studies
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Atrial Fibrillation Management and Outcomes
- Epigenetics and DNA Methylation
- Cardiovascular Function and Risk Factors
- Cancer, Lipids, and Metabolism
- Genetic factors in colorectal cancer
- Radiomics and Machine Learning in Medical Imaging
- Cardiac Valve Diseases and Treatments
- Renal cell carcinoma treatment
- Molecular Biology Techniques and Applications
- Cardiovascular Syncope and Autonomic Disorders
- Economic Theory and Institutions
- Acute Myocardial Infarction Research
- Economic Theory and Policy
- Adolescent Sexual and Reproductive Health
- Cardiac Imaging and Diagnostics
- Cardiomyopathy and Myosin Studies
- Digital and Traditional Archives Management
Fred Hutch Cancer Center
2021-2025
The Ohio State University
1963-2025
East Tennessee State University
1979-2011
James H. Quillen VA Medical Center
2011
Royal Victoria Hospital
1985-1997
McGill University
1985-1997
University of Massachusetts Chan Medical School
1991
Johnson City Medical Center
1990
Medical Center Hospital
1990
Patton State Hospital
1978
The technique of recording His bundle electrograms in man by a tripolar electrode catheter was applied study patients with first, second, and third-degree heart block. In all first-degree block (congenital, acquired, or induced atrial pacing), the delay A-V conduction occurred proximal to as evidenced prolonged P-H interval. enhancement (shortening interval) due isoproterenol qualitatively similar three forms cases Wenckebach phenomenon interval progressively increased until dropped beat...
Abstract Cell-free DNA (cfDNA) has the potential to inform tumor subtype classification and help guide clinical precision oncology. Here we develop Griffin, a framework for profiling nucleosome protection accessibility from cfDNA study phenotype of tumors using as low 0.1x coverage whole genome sequencing data. Griffin employs GC correction procedure tailored variable fragment sizes, which generates better representation chromatin improves accuracy cancer detection classification. We...
Preeclampsia is characterized by placental dysfunction and results in significant morbidity, but reliable early prediction remains challenging. We investigated whether clinically obtained prenatal cell-free DNA (cfDNA) screening (PDNAS) using whole-genome sequencing (WGS) data can be leveraged to predict preeclampsia risk pregnancy (≤16 weeks). Using 1,854 routinely collected clinical PDNAS samples (median, 12.1 weeks) with low-coverage (0.5×) WGS data, we developed a framework quantify...
The exon junction complex (EJC) deposited upstream of mRNA junctions shapes structure, composition, and fate spliced ribonucleoprotein particles (mRNPs). To achieve this, the EJC core nucleates assembly a dynamic shell peripheral proteins that function in diverse post-transcriptional processes. illuminate consequences composition change, we purified EJCs from human cells via RNPS1 CASC3. We show originates as an SR-rich mega-dalton-sized RNP contains but lacks Sometime before or during...
Article22 April 2022Open Access Source DataTransparent process Mammalian UPF3A and UPF3B can activate nonsense-mediated mRNA decay independently of their exon junction complex binding Zhongxia Yi orcid.org/0000-0002-6360-881X Center for RNA Biology, The Ohio State University, Columbus, OH, USA Department Molecular Genetics, Contribution: Conceptualization, Investigation, Writing - original draft, review & editing Search more papers by this author René M Arvola orcid.org/0000-0002-5778-0496...
Advanced prostate cancers comprise distinct phenotypes, but tumor classification remains clinically challenging. Here, we harnessed circulating DNA (ctDNA) to study phenotypes by ascertaining nucleosome positioning patterns associated with transcription regulation. We sequenced plasma ctDNA whole genomes from patient-derived xenografts representing a spectrum of androgen receptor active (ARPC) and neuroendocrine (NEPC) cancers. Nucleosome transcriptional activity were reflected in at regions...
In 20 subjects the effects of controlled premature atrial stimulation on atrioventricular conduction was studied using an electrode catheter technic for recording electrical activity specialized conducting fibers. The least common type response observed that in which A-V delay limited only to nodal region (type A). This obtained whenever increased direct proportion prematurity a propagated impulse. It characterized by progressive prolongation P-H interval with ultimate failure occurring...
In eukaryotic cells, proteins that associate with RNA regulate its activity to control cellular function. To fully illuminate the basis of function, it is essential identify such RNA-associated proteins, their mode action on RNA, and preferred targets binding sites. By analyzing catalogs human defined by ultraviolet light (UV)-dependent -independent approaches, we classify these into two major groups: (i) widely recognized (RBPs), which bind directly UV-crosslink efficiently (ii) a new group...
Seven patients with atrial fibrillation and six flutter were studied using the technic of His bundle recordings. All 13 received digitalis. In each QRS complex (except for premature ventricular beats) was preceded by a single deflection. Complete block distal to not observed. Thus, zone concealment could be localized region proximal bundle. The clinical findings confirmed in three animal experiments. nonconducted P waves also blocked aberrant beats distinguished from presence preceding...
Abstract The Exon Junction Complex (EJC) deposited upstream of exon-exon junctions during pre-mRNA splicing in the nucleus remains stably bound to RNA modulate mRNA fate at multiple post-transcriptional steps until its disassembly translation. Here, we investigated two EJC mechanisms human embryonic kidney 293 (HEK293) cells, one mediated by PYM1, a factor that can bind both ribosome and RBM8A/MAGOH heterodimer core, another elongating itself. We find EJCs lacking PYM1 interaction show no...
Many post-transcriptional mechanisms operate via mRNA 3′UTRs to regulate protein expression, and such controls are crucial for development. We show that homozygous mutations in two zebrafish exon junction complex (EJC) core genes rbm8a magoh leads muscle disorganization, neural cell death, motor neuron outgrowth defects, as well dysregulation of mRNAs subjected nonsense-mediated decay (NMD) due translation termination ≥ 50 nts upstream the last exon-exon junction. Intriguingly, we find...
Although recurrent ventricular tachycardia can be controlled in many patients with standard doses of procainamide hydrochloride or quinidine sulfate, the occasional patient a condition refractory to this therapy has been treated beta- adrenergic blocking agents, cardiac sympathectomy, pacemakers. In four tachycardia, 7.5 10.5 gm successfully arrhythmia without adverse sequelae. Physicians should consider large before they utilize more radical therapy.
The motion of the posterior wall normal left atrium has not been studied systematically. superoposterior portion is adynamic throughout cardiac cycle, whereas inferoposterior displaced posteriorly with atrial filling during ventricular systole. In present study, diameter (LAD), systolic (LASM) and velocity (LASV), were determined in following groups patients: 34 normals; eight patients either coronary artery disease or aortic stenosis; six insufficiency; three septal defect. results obtained...
Abstract Cell-free DNA (cfDNA) has the potential to inform tumor subtype classification and help guide clinical precision oncology. Here we developed Griffin, a new method for profiling nucleosome protection accessibility from cfDNA study phenotype of tumors using as low 0.1x coverage whole genome sequencing (WGS) data. Griffin employs novel GC correction procedure tailored variable fragment sizes, which improves prediction chromatin accessibility. achieved excellent performance detecting in...