In this work, physicochemical properties of two globular proteins—bovine serum albumin (BSA) having a molecular weight 67 kDa and human (HSA) 69 kDa—were characterized. The bulk characteristics these proteins involved the diffusion coefficient (hydrodynamic radius), electrophoretic mobility, dynamic viscosity as function protein solution concentration for various pH values. hydrodynamic radius data suggested an association molecules, most probably forming compact dimers. Using diameter...

Adsorption of fibrinogen from aqueous solutions on mica was studied using AFM and in situ streaming potential measurements. In the first stage, bulk physicochemical properties substrate were characterized for various ionic strength pH. The zeta number uncompensated (electrokinetic) charges protein surfaces determined microelectrophoretic Analogously, measurements, electrokinetic charge density pH range 3−10 NaCl background electrolyte concentration 10−3 10−2 M. Next, kinetics adsorption at...

Bulk physicochemical properties of bovine plasma fibrinogen (Fb) in electrolyte solutions were characterized. These comprised determination the diffusion coefficient (hydrodynamic radius), electrophoretic mobility, and isoelectric point (iep). The hydrodynamic radius Fb for ionic strength 0.15 M was 12.7 nm pH 7.4 (physiological conditions) 12 9.5. Using these values, number uncompensated (electrokinetic) charges on protein Nc calculated from mobility data. It found that physiological...

Vimentin, a protein that builds part of the cytoskeleton and is involved in many aspects cellular function, was recently identified as cell surface attachment site for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The present study investigated physicochemical nature binding between SARS-CoV-2 S1 glycoprotein receptor domain (S1 RBD) human vimentin using atomic force microscopy quartz crystal microbalance. molecular interactions RBD proteins were quantified monolayers...

Adsorption of fibrinogen was theoretically studied using the three-dimensional random sequential adsorption (RSA) model. Fibrinogen molecule shape approximated by bead model considering presence flexible side arms. Various cases were considered inter alia, side-on mechanisms and simultaneous side-on/end-on mechanism. The latter is pertinent to at lower pH (below isoelectric point 5.8) where entire positively charged. Extensive calculations enabled one determine jamming surface concentration...

Adsorption kinetics of human serum albumin (HSA) on silica substrates was studied using optical waveguide lightmode spectroscopy (OWLS) and quartz microbalance (QCM) techniques. Measurements were performed at pH 3.5, 5.6, 7.4 for various bulk suspension concentrations ionic strengths. The diffusion coefficient measurements showed that 3.5 the HSA molecules are stable NaCl from 10–3 to 0.15 M. This allowed us precisely determine mass transfer rate coefficients OWLS QCM cells. experimental...

Abstract Brain-derived neurotrophic factor (BDNF) is essential for the development and function of human neurons, therefore it a promising target neurodegenerative disorders treatment. Here, we studied BDNF-based electrostatic complex with dendrimer nanoparticles encapsulated in polyethylene glycol (PEG) neurotoxin-treated, differentiated neuroblastoma SH-SY5Y cells, model mechanisms. PEG layer was adsorbed at dendrimer-protein core to decrease their cellular uptake reduce BDNF-other...

Molecular dynamics modeling was applied to predict chitosan molecule conformations, the contour length, gyration radius, effective cross-section and density in electrolyte solutions. Using various experimental techniques diffusion coefficient, hydrodynamic diameter electrophoretic mobility of molecules were determined. This allowed calculate zeta potential, electrokinetic charge ionization degree as a function pH temperature. The solution zero shear dynamic viscosity also measured, which...

The toxicity of silver nanoparticles (AgNPs) depends on their physicochemical properties. ongoing research aims to develop effective methods for modifying AgNPs using molecules that enable control over the processes induced by in both normal and cancerous cells. Application amino acid-stabilized appears promising, exhibiting tunable electrokinetic Therefore, this study focused determining influence surface charge cysteine (CYS)-stabilized towards human B (COLO-720L) T (HUT-78) lymphocyte...

The adsorption kinetics of human serum fibrinogen at silica substrates was studied using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance (QCM) techniques. Measurements were performed pH 3.5, 4, 7.4 for various ionic strengths. experimental data interpreted in terms a hybrid random sequential model. This allowed the mass transfer rate coefficient OWLS cell maximum coverages to be determined pHs. appearance different, pH-dependent mechanisms on confirmed. At 3.5...

Adsorption of poly(amido amine) dendrimers (G8 generation) on silica substrates was studied by atomic force microscopy (AFM), quartz crystal microbalance (QCM), and optical waveguide lightmode spectroscopy (OWLS). Initially, diffusion coefficient, hydrodynamic diameter, electrophoretic mobility, stability the were determined as a function pH ionic strength for various electrolytes. kinetics measurements performed AFM allowed to determine porosity their internal solvation Subsequent carried...

Physicochemical properties of bovine plasma fibrinogen (Fb) in electrolyte solutions were characterized. These comprised the diffusion coefficient (hydrodynamic radius), determined by DLS method, electrophoretic mobility and isoelectric point. The hydrodynamic radius Fb was 12 nm for pH<5. number uncompensated (electrokinetic) charges on protein N c calculated from data. It found that pH<5.8 electrokinetic charge positive, independently ionic strength negative pH>5.8. For pH=3.5 value , 26...

Colloid particle deposition was applied to characterize bovine and human fibrinogen (Fb) monolayers on mica produced by controlled adsorption under diffusion transport at pH 3.5. The surface concentration of Fb determined AFM enumeration single molecules adsorbed over the substrate surface. electrokinetic properties for various ionic strength were studied using in situ streaming potential measurements. It shown that adsorbs irreversibly a broad range 4 × 10(-4) 0.15 M, NaCl. overcharging...

An efficient method was developed enabling the synthesis of spheroidal polymer microparticles. Thorough physicochemical characteristics particles were acquired comprising size, shape, electrophoretic mobility, and diffusion coefficient. The monodisperse, their shape well-fitted by prolate spheroids having axis ratio equal to 4.17. Knowing coefficient, hydrodynamic diameter 449 nm calculated, which matched value derived from Brenner's analytical expression. Particle deposition kinetics on...

The surface properties of polyelectrolyte multilayers (PEMs) obtained <italic>via</italic> sequential adsorption oppositely charged polyions from their solutions and used as cushions for supported lipid bilayers were investigated.