Cancer stem cells (CSCs) have been associated with metastasis and therapeutic resistance can be generated via epithelial mesenchymal transition (EMT). Some studies suggest that the hormone melatonin acts in CSCs may participate inhibition of EMT. The objectives this study were to evaluate formation mammospheres from canine human breast cancer cell lines, CMT-U229 MCF-7, effects treatment on modulation EMT molecular markers: OCT4, E-cadherin, N-cadherin vimentin, as well viability...

Liver cancer is the sixth most commonly occurring globally, and main histological type hepatocellular carcinoma. This of neoplasia has a poor prognosis due to high rate recurrence intrahepatic metastasis, which are closely associated with angiogenic process. Vascular endothelial growth factor (VEGF), under control hypoxia inducible factor-1α (HIF-1α), stimulates proliferation cells increases cell permeability, promoting growth, spread metastasis tumors. Melatonin, hormone secreted by pineal...

Background: Oral cancer is the most common type of head and neck its high rate mortality morbidity closely related to processes angiogenesis tumor metastasis. The overexpression pro-angiogenic genes, HIF-1α VEGF, pro-metastatic gene, ROCK-1, are associated with unfavorable prognosis in oral carcinoma. Melatonin has oncostatic, antiangiogenic antimetastatic properties several types neoplasms, although relationship been little explored. This study aims analyze expression genes HIF-1α, VEGF...

NF-kB (nuclear factor kappa B) is a transcription composed of two subunits, p50 and p65, which plays key role in the inflammatory process. Melatonin has oncostatic, antiangiogenic antimetastatic properties, some recent studies have indicated an inhibitory effect melatonin on types cancer. This work aims to investigate effects treatment expression NFkB breast liver cancer models.The xenographic model was performed using female Balb/c nude athymic mice injected with MDA-MB-231 cells. The...

Oncogenic human papillomavirus (HPV), a causative agent of uterine cervical cancer, has also been detected in head and neck squamous cell cancers, especially carcinomas the tonsils. However, true HPV prevalence normal neoplasic oropharyngeal mucosa remains uncertain. To determine DNA cancer-free individuals, study was carried out on 50 Brazilian subjects. PCR performed to identify samples from four sites oropharynx (tonsils, soft palate, base tongue, back wall pharynx). For amplification...

AIM:To test the hypothesis that, in Southeastern Brazilian population, GSTT1, GSTM1 and CYP2E1 polymorphisms putative risk factors are associated with an increased for gastric cancer. METHODS:We conducted a study on 100 cases of cancer (GC), chronic gastritis (CG), 150 controls (C).Deletion GSTT1 genes was assessed by multiplex PCR.CYP2E1/PstI genotyping performed using PCR-RFLP assay. RESULTS:No relationship between GSTT1/GSTM1 deletion c1/c2 genotype observed among three groups.However,...

O carcinoma de células escamosas cabeça e pescoço (HNSCC) constitui o quinto tipo câncer mais comum mundialmente, com uma incidência anual global 780.000 novos casos. Os sítios comuns incluem cavidade oral, orofaringe, hipofaringe, nasofaringe, nasal, seios paranasais, laringe glândulas salivares. consumo tabaco e/ou álcool são os principais fatores risco envolvidos no desenvolvimento do HNSCC. Apesar dos recentes avanços tratamento, índice sobrevivência pacientes HNSCC tem permanecido em...

Breast cancer is the most common among women and has a high mortality rate. Adverse conditions in tumor microenvironment, such as hypoxia acidosis, may exert selective pressure on tumor, selecting subpopulations of cells with advantages for survival this environment. In context, therapeutic agents that can modify these conditions, consequently intratumoral heterogeneity need to be explored. Melatonin, addition its physiological effects, exhibits important anti-tumor actions which associate...

Breast cancer (BC) is the malignant neoplasm with highest mortality rate in women and female dogs are good models to study BC. We investigated efficacy of liquid biopsy detect gene mutations diagnosis follow-up In this study, 57 37 BC samples were collected from dogs, respectively. After core plasma collected, DNA ctDNA tumor fragments processed for next generation sequencing (NGS) assay. preprocessing data, they submitted Genome Analysis ToolKit (GATK). women, 1788 variants identified 221...

In several DNA repair genes, polymorphisms may result in reduced capacity, which has been implicated as a risk factor for various types of cancer. The frequency the polymorphic alleles varies among populations, suggesting an ethnic distribution genotypes. We genotyped 300 healthy Southeastern Brazilian individuals (262 European ancestry and 38 African ancestry) codons 194 399 XRCC1 base excision pathway gene codon 241 XRCC3 homologous recombination gene. allele frequencies were 0.07...

The ZNF706 gene encodes a protein that belongs to the zinc finger family of proteins and was found be highly expressed in laryngeal cancer, making structure function worthy investigation. In this study, we purified recombinant human suitable for structural analysis Escherichia coli BL21(DH3).ZNF706 mRNA extracted from larynx tissue sample, cDNA ligated into cloning vector using TOPO method. according E. expression system procedures nickel-affinity column. qualities quantification alpha, beta...

Mammary cancer is the most prevalent type of in female dogs. The main cause mortality occurrence metastasis. metastatic process complex and involves Epithelial- Mesenchymal Transition (EMT), which can be activated by Transforming Growth Factor beta (TGF-β) changes cellular phenotype, as well as, expression proteins such E-cadherin, N-cadherin, vimentin claudin-7. Melatonin a hormone with oncostatic anti-metastatic properties appears to participate TGF-β pathway. Thus, present work aimed...

The breast cancer (BC) has a high rate of morbidity and mortality; thus, the discovery new therapeutic targets is great interest for researchers. Previous studies have documented that melatonin, main hormone synthesized by pineal gland, plays important roles in control tumorigenesis. Similar to agomelatine, melatonin analogue can also perform its functions binding G protein coupled membrane receptors MT1 MT2. In series carried out two cell lines (MCF-7 MDA-MB-231 strains), dose-responsive...

Mammary tumours (MT) are one of the most prevalent malignancies in female dogs and women. Currently, molecular analyzes have shown that each tumour type presents its own genetic signature. In this context, liquid biopsy allows a comprehensive characterisation tumour, enabling early diagnosis personalised treatment patients. women, deleterious mutations inherited BRCA2 gene associated with an increased risk breast cancer, resistance to therapies worse prognosis. dogs, there many divergent...

Breast cancer is the most prevalent tumor type among women and female dogs. Tumor malignancy characterized by epithelial-to-mesenchymal transition (EMT) which leads to metastasis formation. The inhibition of angiotensin II I receptor (AGTR1) an antagonist such as losartan can suppress angiogenesis, consequently contributing control. aim this study was analyze capacity AGTR-1 gene edition modulate EMT process in triple negative/metastatic mammary cells, compared existing treatment protocols...

The second main cause of death in Brazil is cancer, and according to statistics disclosed by National Cancer Institute from (INCA) 466,730 new cases cancer are forecast for 2008. analysis tumour tissues various types patients' clinical data, genetic profiles, characteristics diseases epidemiological data may lead more precise diagnoses, providing effective treatments. In this work we present a decision support system diseases, which manages relational database containing information relating...

Background: The most aggressive breast cancer is the triple negative histological type, and gold standard for its treatment platinum salts, such as carboplatin. Due to high recurrence, there a need test new drugs, PARP inhibitors (PARPi), that induce lethality in cells with DNA damage. Olaparib PARPi, already used some tumors but not tested canine species. Thus, aim of this study was demonstrate efficacy olaparib inhibiting repair control disease progression by decreasing migration capacity...

Breast cancer (BC) presents high mortality rate and about 30-40% have mutation in the PIK3CA gene. Alpelisib is a PI3K inhibitor that acts on p110α. The melatonin shown important anti-neoplastic effects may increase effectiveness of chemotherapy. This study evaluated synergistic action Melatonin BC lines carrying H1047R PIK3CA, relative to cellular dynamics PI3K/AKT/mTOR pathway. MDA-MB-468 (triple negative), MDA-MB-453 negative, PIK3CA) T-47D cells (luminal A, were divided into four...