A5102725381- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Toxoplasma gondii Research Studies
- Viral-associated cancers and disorders
- Parvovirus B19 Infection Studies
- Chemokine receptors and signaling
- Inflammatory mediators and NSAID effects
- Virus-based gene therapy research
- Mycobacterium research and diagnosis
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Respiratory viral infections research
- Adenosine and Purinergic Signaling
- Polyomavirus and related diseases
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Systemic Lupus Erythematosus Research
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Viral Infections and Vectors
- RNA regulation and disease
- HIV Research and Treatment
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- Photosynthetic Processes and Mechanisms
University Hospital Ulm
2010-2023
Universität Ulm
1997-2021
National Center for Global Health and Medicine
2016
German Center for Infection Research
2016
Weatherford College
2009-2013
Centre Hospitalier Universitaire Sainte-Justine
2004
Charité - Universitätsmedizin Berlin
2003
Institute of Virology of the Slovak Academy of Sciences
2003
Goethe University Frankfurt
2003
KU Leuven
2002
Human cytomegalovirus (HCMV) is a widely spread herpesvirus, suggested to play role in tumor progression. US28, chemokine receptor encoded by HCMV, binds broad spectrum of chemokines and constitutively activates various pathways linked proliferation. Our studies reveal that expression US28 induces proangiogenic transformed phenotype up-regulating the vascular endothelial growth factor enhancing cell cycle US28-expressing cells promote tumorigenesis when injected into nude mice. The G...
BACKGROUND: Cytomegalovirus (CMV) disease constitutes a serious complication after allogeneic stem cell transplantation. For the clearance of CMV, CD8+ T cells are pivotal. STUDY DESIGN AND METHODS: Here, novel streptamer technology was used at good manufacturing practice (GMP) level for adoptive transfer CMV‐specific into acute leukemia patients with recurrent high CMV antigenemia RESULTS: After single transfusion, frequency CD8+CD45RA+CCR7– effector increased dramatically from 0.0% to...
The human cytomegalovirus (HCMV), potentially associated with the development of malignancies, encodes constitutively active chemokine receptor US28. Previously, we have shown that US28 expression induces an oncogenic phenotype both in vitro and vivo. Microarray analysis revealed differential genes involved signaling US28-expressing NIH-3T3 cells. In particular, cyclooxygenase-2 (COX-2), a key mediator inflammatory diseases major determinant several forms cancer, was highly up-regulated....
The efficacy of different letermovir (AIC246, MK8228) doses (60, 120, and 240 mg/day) against human cytomegalovirus (HCMV) was evaluated in a recent phase 2b dose-range-finding prophylaxis study stem-cell transplant recipients. Here we report the genotypic phenotypic characterization 15 viral breakthroughs considered to be virological failures. Direct sequencing an HCMV open reading frame UL56 region that included amino acids 230–370 thus encompassed all known resistance mutations followed...
Until recently, cytomegalovirus (CMV) infection represented a major threat to lung transplant recipients. Preliminary studies have shown that antiviral prophylaxis might improve the outcome for these patients.We extended our initial pilot trial of prolonged with either oral ganciclovir (1 g 3 times per day) or valganciclovir (450 mg twice day). The included 96 patients who were at risk CMV-related events.CMV resulted in significant decrease events (i.e., active and disease), from 75% control...
The compound letermovir (LMV) has recently been approved for the prophylaxis of cytomegalovirus (CMV) infection and disease in adult CMV seropositive recipients an allogeneic hematopoietic stem cell transplant. LMV inhibits replication by binding to viral terminase complex. However, first cases clinical resistance have occurred. Here we report a fast breakthrough resistant during secondary hematopoietic-cell transplant recipient. A 44-year-old male patient with acute myeloid leukemia (AML)...
Miller-Fisher syndrome (MFS) is classified as a variant of Guillain-Barré (GBS), accounting for 5%-25% all GBS cases. Since the coronavirus disease-2019 (COVID-19) outbreak, increasing evidence has been reported neurological manifestations severe acute respiratory 2 (SARS-CoV-2) infection, affecting both central and peripheral nervous system. Here we report clinical course, detailed cerebrospinal fluid (CSF) profile including CSF/blood antibody status, neurochemical characteristics patient...
The temporal expression of the UL97 gene product during human cytomegalovirus (HCMV) infection foreskin fibroblasts (HFF) and subcellular localization this protein were analyzed by using a polyclonal antiserum raised against truncated 47 kDa. was detectable 16 h after Western blot (immunoblot) analysis. Since only reduced occurred in presence two inhibitors DNA replication, phosphonoacetic acid ganciclovir, we conclude that is an early-late gene, requiring replication for maximum expression....
Summary The Glossy2 (Gl2) locus of maize is required for the formation epicuticular wax layer young plants. gl2 mutant seedlings can be visually identified because their glossy leaf surface which different from dull wild‐type seedlings. Gl2 was isolated by transposon tagging. Seven unstable mutations, gl2‐m2 to gl2‐m8 , were induced in a parental strain carrying an active transposable Activator (Ac) element wx‐m7 allele. Genetic tests on allele indicated that it not caused Ac but insertion...
The human cytomegalovirus (HCMV) UL33 gene is conserved among all β-herpesviruses and encodes a protein that shows sequence similarity with chemokine receptors belonging to the family of G protein-coupled receptors. Here, we show HCMV predominantly transcribed as spliced mRNA which 5′ terminus localized 55 bp upstream start codon. Like its homolog from rat (RCMV), R33, activates multiple signaling pathways in ligand-independent manner. Although both constitutively activate phospholipase C...
The resurrection plant Craterostigma plantagineum can recover from severe desiccation within 24 h of contact with water, and it is used as a model system to analyse tolerance in higher plants. During drying or ABA treatment specific set transcripts accumulates rapidly leaves other tissues. In order study transcriptional mechanisms stress‐induced gene expression one (CDeT27‐45) was selected for promoter analysis. Chimeric fusions were constructed the CDeT27‐45 β‐glucuronidase luciferase....
ABSTRACT We have investigated the morphogenesis of human and murine cytomegalovirus by transmission electron microscopy after high-pressure freezing, freeze substitution, plastic embedding. observed large tubular infoldings inner nuclear membrane that were free lamina active in primary envelopment subsequent transport capsids to periphery. Semiquantitative determinations enlarged area location nucleocapsids demonstrated this structure represents a virus-induced specialized domain at which...
CMV associated tissue-invasive disease is with a considerable risk of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (HSCT). Recently, the terminase inhibitor letermovir (LMV) has been approved for prophylaxis infection in HSCT. We hereby report 60-year-old female experiencing reactivation HSCT seronegative donor-constellation. Due to ongoing elevated viral load drug-associated myelosuppression, which prevented ganciclovir therapy, treatment was replaced by...
Human cytomegalovirus (HCMV) is a human pathogenic herpesvirus associated with variety of clinical symptoms. Current antiviral therapy not always effective, so that improved drug classes and drug-targeting strategies are needed. Particularly host-directed antivirals, including pharmaceutical kinase inhibitors (PKIs), may help to overcome problems resistance. Here, we focused on utilizing selection clinically relevant PKIs determined their anticytomegaloviral efficacies. Particularly,...
ABSTRACT An open reading frame (ORF), US28, with homology to mammalian chemokine receptors has been identified in the genome of human cytomegalovirus (HCMV). Its protein product, pUS28, shown bind several CC chemokines, including RANTES, MCP-1, and MIP-1α, CX 3 C fractalkine high affinity. Addition chemokines cells expressing pUS28 was reported cause a pertussis toxin-sensitive increase concentration cytosolic free Ca 2+ . Recently, mediate constitutive, ligand-independent, toxin-insensitive...
Zika virus (ZIKV) is an emerging pathogen that causes congenital infections which may result in birth defects, such as microcephaly. Currently, no approved treatment or vaccination available. ZIKV can be readily detected cell culture where virally infected cells are normally stained by specific antibodies. As regularly a cytopathic effect, we were wondering whether this viral property used to quantitatively determine infectivity. We here describe the use of...
Cytomegalovirus (CMV) disease represents a serious complication after allogeneic peripheral blood stem cell (PBSC) transplantation. If possible, donors for transplantation are selected on the basis of their CMV serostatus. However, cytomegalovirus-specific immune status can be further characterized by measuring phosphoprotein 65-specific CD8(+) T frequencies using tetramers, pentamers, and streptamers. We therefore investigated specificity sensitivity all 3 methods compared results to...