A5069789302- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Renal and related cancers
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Genetic Syndromes and Imprinting
- Birth, Development, and Health
- Mesenchymal stem cell research
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Gender, Sexuality, and Education
- Genetic and phenotypic traits in livestock
- Galectins and Cancer Biology
- Business, Education, Mathematics Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Violence, Education, and Gender Studies
- Criminal Justice and Penology
- Tissue Engineering and Regenerative Medicine
- Psychology and Mental Health
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Social Sciences and Policies
- Accounting and Financial Management
Universidade Federal de Uberlândia
2016-2024
Brazilian Agricultural Research Corporation
2018-2024
Abstract This study aimed to evaluate the effects of donor age on lipid metabolism during in vitro maturation (IVM) pigs cumulus‐oocyte complexes (COCs). We evaluated transcript levels genes, percentage ooplasm occupied by droplets (LD) and DNA methylation COCs from sows prepubertal gilts. Transcript six genes ( ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2 ), which were analyzed cumulus cells (CCs), increased after 44 h IVM sow group. In gilt group, only FASN expression increased, while NR3C1...
Cloning using somatic cell nuclear transfer (SCNT) has many potential applications such as in transgenic and genomic-edited animal production. Abnormal epigenetic reprogramming of nuclei is probably the major cause low efficiency associated with SCNT. Strategies to alter DNA donor may help improve cloning efficiency. In present study, we aimed characterize effects procaine S-adenosyl-l-homocysteine (SAH) demethylating agents during culture bovine skin fibroblasts. We characterized SAH on...
The establishment of epigenetic marks during the reprogramming window is susceptible to environmental influences, and stimuli this critical stage can cause altered DNA methylation in offspring. In a previous study, we found that low levels sulphur cobalt (low S/Co) diet offered oocyte donors methylome bovine embryos. However, due extensive occurs embryogenesis, hypothesized different regions (DMRs) identified blastocysts may not maintain adulthood. Here, aimed characterize DMRs previously...
Abstract Somatic cell nuclear transfer (SCNT) is commercially used despite incomplete reprogramming of the somatic nucleus by enucleated oocyte compromising its efficiency. Oocyte selection a key factor in increasing this efficiency as cytoplasm reprograms differentiated cell. In study, we adapted methodology to characterize epialleles potential epigenetic markers single vitro matured oocytes. Characterization regions that control expression imprinted genes, X‐chromosome inactivation, and...
The purpose of this study was to characterize the reproductive physiology, oocyte competence, and chromatin compaction in Nelore calves early-prepubertal period (EPP) intermediate-prepubertal (IPP). Calves aged 2–5 8–11 months old (IPP) were assigned Trial 1 (morpho-physiological–endocrine evaluations, n = 8) or 2 (oocyte donors, vs. respective control groups cows (n 8, each). All morphological endpoints, except antral follicle count, increased from EPP IPP. LH-FSH plasma concentrations...
Abstract Maternal nutrition is critical in mammalian development, influencing the epigenetic reprogramming of gametes, embryos, and fetal programming. We evaluated effects different levels sulfur (S) cobalt (Co) maternal diet throughout pre- periconceptional periods on biochemical reproductive parameters donors DNA methylome progeny Bos indicus cattle. The low-S/Co group differed from control with respect to homocysteine, folic acid, B 12 , insulin growth factor 1, glucose. oocyte yield was...
ABSTRACT: Somatic Cell Nuclear Transfer (SCNT-Cloning) is a promising technique in many areas and based on genetically identical individuals. However, its efficiency low. Studies suggest that the leading cause inadequate epigenetic reprogramming. This study aimed to characterize methylation pattern of exon 10 regions IGF2 gene Imprinting Control Region (ICR) H19 placenta cloned calves. For this study, female male calves presenting different phenotypes were used. Genomic DNA from these...
X chromosome inactivation (XCI) compensates for the imbalance in gene expression between sexes.In mice, it is well established that long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) essential initiating XCI.The most well-known antisense of mouse XIST locus TSIX, a negative modulator XIST.However, cattle, these events are not yet established.In this study, we characterized patterns strand-specific transcription along bovine fetal placenta, since understanding regulation...
O jogo “Que ervilha sou eu?” permite a mobilização dos conceitos da Genética Mendeliana de modo ativo e com eficiência. Para jogar, é necessário entender as relações dominância recessividade alelos, proporções diferentes fenótipos na descendência relação entre genótipo fenótipo.
BL and HB.The expression of XIST H2AFY2 reached the lowest levels at D11 D14.We suggest that changes in mRNA these genes initial stages development are related to onset XCI cattle.This study is relevant improve our understanding dynamic mechanisms act cattle until elongated embryo stage.
Abstract Cloning using somatic cell nuclear transfer (SCNT) has many potential applications such as in transgenic and genomic-edited animal production. Abnormal epigenetic reprogramming of nuclei is probably the major cause low efficiency associated with SCNT. Strategies to alter DNA donor may help improve cloning efficiency. In present study, we aimed characterise effects procaine S-adenosyl-L-homocysteine (SAH) demethylating agents during culture bovine skin fibroblasts. We characterised...