A5062686144- RNA Research and Splicing
- Toxoplasma gondii Research Studies
- Cardiomyopathy and Myosin Studies
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Genetic Neurodegenerative Diseases
- Sports Performance and Training
- Fluid Dynamics and Heat Transfer
- Molecular spectroscopy and chirality
- Muscle metabolism and nutrition
- Mass Spectrometry Techniques and Applications
- Genetics and Physical Performance
- Medical Imaging Techniques and Applications
- Hormonal and reproductive studies
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Exercise and Physiological Responses
- thermodynamics and calorimetric analyses
- Chemical Thermodynamics and Molecular Structure
Human Technopole
2024
Max Perutz Labs
2016-2021
Vienna Biocenter
2019-2021
University of Vienna
2016-2021
MORN (Membrane Occupation and Recognition Nexus) repeat proteins have a wide taxonomic distribution, being found in both prokaryotes eukaryotes. Despite this ubiquity, they remain poorly characterised at structural functional level compared to other common repeats. In terms, are often assumed be lipid-binding modules that mediate membrane targeting. We addressed putative activity by focusing on protein composed solely of repeats— Trypanosoma brucei MORN1. Surprisingly, no evidence for...
Sarcomeric proteins FATZ-1 and α-actinin-2 associate in a tight fuzzy complex, which forms phase-separated condensates.
ABSTRACT MORN (Membrane Occupation and Recognition Nexus) repeat proteins have a wide taxonomic distribution, being found in both prokaryotes eukaryotes. Despite this ubiquity, they remain poorly characterised at structural functional level compared to other common repeats. In terms, are often assumed be lipid-binding modules that mediate membrane targeting. We addressed putative activity by focusing on protein composed solely of repeats – Trypanosoma brucei MORN1. Surprisingly, no evidence...
Actinin-2 plays a central role in Z-disk assembly and stability as it crosslinks actin filament thanks to its unique antiparallel dimeric architecture.The organization spacing between each -actinin-2 dimer is regulated via two specific interactions with titin, the giant blueprint protein of sarcomere: (i) EF3-4 interact titin Z-repeats PIP2-regulated manner (ii) rod domain interacts Zq titin.The first interaction has been studied detail at all levels, but molecular basis behind second well...
Actinin-2 plays a pivotal role in Z-disk assembly and stability as it crosslinks actin filaments from adjacent sarcomeres acts binding platform for number of proteins.Among them is FATZ-1, 30-kDa protein that appears when Z-bodies, the precursors Z-disks, are formed.Accordingly, FATZ-1 believed to have central at initial stages myofibrillogenesis by serving focal point interactions with proteins, but its structure function remain unknown.Here, we used bioinformatics, CD SAXS show...
In sarcomeres, α-actinin crosslinks actin filaments and anchors them to the Z-disk.FATZ proteins interact with five other core Z-disk proteins, contributing myofibril assembly maintenance as a protein interaction hub.Here we report first structure its cellular validation of α-actinin-2 in complex partner, FATZ-1, which is best described conformational ensemble.We show that FATZ-1 forms tight fuzzy propose molecular mechanism via main recognition elements secondary binding sites.The obtained...