J. W. Randles

ORCID: 0000-0003-2231-816X
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About
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Research Areas
  • Plant Virus Research Studies
  • Plant and Fungal Interactions Research
  • Insect-Plant Interactions and Control
  • Plant Disease Resistance and Genetics
  • Plant Pathogenic Bacteria Studies
  • Plant-Microbe Interactions and Immunity
  • Coconut Research and Applications
  • Oil Palm Production and Sustainability
  • Plant tissue culture and regeneration
  • Plant Pathogens and Fungal Diseases
  • Phytoplasmas and Hemiptera pathogens
  • Cocoa and Sweet Potato Agronomy
  • Transgenic Plants and Applications
  • Bacteriophages and microbial interactions
  • Legume Nitrogen Fixing Symbiosis
  • Agronomic Practices and Intercropping Systems
  • Animal Virus Infections Studies
  • Agricultural pest management studies
  • Plant Reproductive Biology
  • CRISPR and Genetic Engineering
  • Viral gastroenteritis research and epidemiology
  • Plant Parasitism and Resistance
  • Date Palm Research Studies
  • Mosquito-borne diseases and control
  • Plant Pathogens and Resistance

The University of Adelaide
2012-2024

Tricoci University of Beauty Culture
2005

Brookhaven College
2005

Agricultural Institute
1974-1993

Heinrich Heine University Düsseldorf
1982-1983

Horticultural Research Institute
1976-1977

Bureau of Plant Industry
1976

Beltsville Agricultural Research Center
1976

South Australian Research and Development Institute
1970

University of Auckland
1968

Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV as bait, confirmed interaction between these vitro. induces expression specifically infected...

10.1105/tpc.104.027235 article EN The Plant Cell 2004-12-17

DNA β is a circular single-stranded satellite which co-infects with certain monopartite helper begomoviruses to cause economically important diseases, such as cotton leaf curl disease (CLCuD). encodes single protein, C1. Tomato New Delhi virus (ToLCNDV) bipartite begomovirus in both A and B are required for systemic infection. Inoculation of tomato plants ToLCNDV alone induced local but not infection, whereas co-inoculation the associated CLCuD resulted containing disrupted C1 open reading...

10.1099/vir.0.83049-0 article EN Journal of General Virology 2007-09-13

Members of the family Avsunviroidae have a single-stranded circular RNA genome that adopts rod-like or branched conformation and can form, in strands either polarity, hammerhead ribozymes involved their replication plastids through symmetrical RNA–RNA rolling-circle mechanism. These viroids lack central conserved region typical members Pospiviroidae. The includes three genera, Avsunviroid , Pelamoviroid Elaviroid, with total four species. This is summary ICTV Report on taxonomy...

10.1099/jgv.0.001045 article EN cc-by Journal of General Virology 2018-03-26

Members of the family Pospiviroidae have single-stranded circular RNA genomes that adopt a rod-like or quasi-rod-like conformation. These contain central conserved region is involved in replication nucleus through an asymmetric RNA–RNA rolling-circle mechanism. lack hammerhead ribozymes are typical viroids classified Avsunviroidae . The includes genera Apscaviroid , Cocadviroid Coleviroid Hostuviroid and Pospiviroid with >25 species. This summary ICTV Report on which available at...

10.1099/jgv.0.001543 article EN Journal of General Virology 2020-12-17

DNA beta is a single-stranded satellite which encodes single gene, betaC1. To better understand the role of betaC1 in pathogenicity beta, yeast two-hybrid screen tomato cDNA library was carried out using from Cotton leaf curl Multan virus (CLCuMV) as bait. A ubiquitin-conjugating enzyme, designated SlUBC3, functionally complemented mutant deficient enzymes identified. The authenticity and specificity interaction between SlUBC3 confirmed both vivo, bimolecular fluorescence complementation...

10.1094/mpmi-22-6-0737 article EN other-oa Molecular Plant-Microbe Interactions 2009-05-15

Promoters isolated from the Tomato leaf curl virus (TLCV) drive both constitutive and tissue-specific expression in transgenic tobacco. Following systemic TLCV infection of plants stably expressing promoter:GUS transgenes, transgene driven by all six promoters was silenced. Silencing coat protein (V2:GUSdeltaC) characterized more detail. Transgene silencing observed leaf, stem, pre-anthesis floral tissue occurred with continued replication host tissues. Infection V2:GUSdeltaC heterologous...

10.1094/mpmi.2003.16.5.429 article EN other-oa Molecular Plant-Microbe Interactions 2003-05-01

SUMMARY Nicotiana velutina mosaic virus (NVMV), found in Australia, was transmitted by inoculation of sap to twenty species the Solanaceae and Chenopodiaceae, Gomphrena globosa; its host range closely resembles that potato mop‐top (PMTV). Infectivity abolished when kept at room temperature between 1 4 days, or heated for 10 min 60 70 °C. NVMV frequently through seed four spp. PMTV were purified a method involved redissolving particles sedimented low speed centrifugation leaf extracts,...

10.1111/j.1744-7348.1976.tb01748.x article EN Annals of Applied Biology 1976-10-01

The six open reading frames of Tomato leaf curl virus (TLCV) were expressed in host Nicotiana species using a Tobacco mosaic vector. Each the genes, except that encoding viral coat protein, produced phenotypic effect when planta, but corresponding untranslatable mutant genes asymptomatic. C1 (Rep) gene invoked hypersensitive response clevelandii restricted construct to sites infection. Expression C2 N. benthamiana necrotic lesions on inoculated leaves as well severe veinal necrosis...

10.1094/mpmi.2004.17.1.27 article EN Molecular Plant-Microbe Interactions 2004-01-01

Summary Fractionation, electroblotting and molecular hybridisation of nucleic acids extracted from tissue African oil palm coconut some other monocotyledonous species, collected in several areas the south‐west Pacific region, demonstrated presence small with nucleotide sequences secondary structure similar to cadang‐cadang viroid (CCCVd). The palms which contained CCCVd‐related molecules showed orange leaf spots resembling those described for naturally infected CCCVd Philippines, also...

10.1111/j.1744-7348.1991.tb06092.x article EN Annals of Applied Biology 1991-02-01

A new virus, subterranean clover mottle virus (SCMoV) was found at several locations in Western Australia Trifolium subterraneum L. (subterranean clover) with severe disease symptoms. Paiticles of the were shown to share many physical properties members sobemo‐virus group. However, addition single‐stranded RNA M J about 1.5 × 10 6 which is characteristic sobemoviruses, SCMoV particles also contained circular and linear viroid‐like RNAs two size classes, one 400 other 300 nucleotides long. At...

10.1111/j.1365-3059.1983.tb01300.x article EN Plant Pathology 1983-03-01
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