A5043370781- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Chromosomal and Genetic Variations
- Animal Genetics and Reproduction
Harvard University
2017-2021
Multiplex genome editing is the simultaneous introduction of multiple distinct modifications to a given genome. Though in its infancy, maturation this field will facilitate powerful new biomedical research approaches and enable host far-reaching biological engineering applications, including therapeutic modalities industrial as well "genome writing" de-extinction efforts. In Perspective, we focus on multiplex large eukaryotic genomes. We describe current state multiplexed editing, limits our...
Abstract We have exploited the repetitive nature of transposable elements human genome to generate synthetic circuits. Transposable such as LINE-1 and Alu successfully replicated in mammalian genomes throughout evolution reach a copy number ranging from thousands more than million. Targeting these with programmable DNA nucleases CRISPR-Cas9 rapidly induce extremely high levels cell death. use this genotoxic feature build biocontainment circuits: CRISPR defense system (CRISPR-DS) capable...
Abstract Bacterial genome editing methods are used to engineer strains for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method of editing, but traditional techniques using endogenous RecA-mediated pathways inefficient laborious. Phage encoded RecT proteins can improve HR over 1000-fold, these have limited portability between species. Using Escherichia coli , Lactococcus lactis, Mycobacterium smegmatis, Lactobacillus rhamnosus Caulobacter...