M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF).To find differences between BALF from affected (clBALF) and hlBALF opposite, healthy lung, as a control, same patient, regarding their individual polarization correlation with IL-10 TGF-β.Eighteen patients confirmed were investigated. Macrophage subtyping was performed...

Regulatory T cells (Tregs) play an important role in the suppression of immune response lung cancer. Cytotoxic T-lymphocyte antigen 4 (CTLA-4) expressed on lymphocytes is capable downregulating cytotoxic and constitutively Tregs. Little known about population Tregs with two forms CTLA-4: surface (s) intracellular (in) cancer environment. Th17 defined by production IL-17 have pleiotropic functions anticancer response. Our aim was to detect elements regulation three compartments: analysis...

Studying the dynamics changes of neutrophils during innate immune response in coronavirus 2019 (COVID-19) can help understand pathogenesis this disease. The aim study was to assess usefulness new neutrophil activation parameters: Immature Granulocyte (IG), Neutrophil Reactivity Intensity (NEUT-RI), Granularity (NEUT-GI), and data relating granularity, activity, volume (NE-WX, NE-WY, NE-WZ) available hematology analyzers distinguish convalescent patients from with active SARS-CoV-2 infection...

Monocytes play a role in viral biology, but little is known about the monocyte subpopulation course of COVID-19 disease. The aim study was analysis classical, intermediate and non-classical monocytes with expression PD-L1 CD62L, TIM-3 CD86 molecules peripheral blood (PB) to distinguish patients SARS-CoV-2 infection from convalescent patients. group consisted 55 51 cells were analyzed by flow cytometry. number proportion lower than We observed ones. There higher PDL-1-positive noticed...

(1) The cells from the monocyte line play an important role as regulators of cancer development and progression. Monocytes present pro- anti-tumor immunity differentiation into macrophages. Macrophages are predominant in lung environment could be evaluated by bronchoalveolar lavage fluid (BALF). (2) aim study was analysis monocytes: classical, intermediate non-classical with expression of: CD62L, CD11c, CD18, HLA-DR non-small cell (NSCLC) their correlation BALF macrophages lungs (clBALF)...

The presence of metastases in mediastinal lymph nodes (LNs) is essential for planning lung cancer treatment and assessing anticancer immune responses. aim the study was to assess LNs neoplastic cells evaluate cancer-selected antigen expression. LN aspirates were obtained during an EBUS/TBNA procedure. analyzed using a hematological analyzer flow cytometry. It possible indicate characterized by high fluorescence connected with metabolic activity determine their non-hematopoietic origin Using...

Lymphocytes play a leading role in regulation of the immune system lung cancer patients. The recognition T cells profile may help prediction effectiveness anticancer immunotherapy. aim study was to determine dominant subpopulation CD4+ and CD8+ lymphocytes metastatic non-metastatic lymph nodes (LNs) LNs aspirates were obtained during EBUS/TBNA procedure analyzed by flow cytometry. We showed higher percentage effector memory than (28.6 vs. 15.3% 28.6 14.0%, p< 0.05). proportion CD45RO+...

The overexpression of programmed death-1 (PD-1) and cytotoxic T cell antigen 4 (CTLA-4) receptors on cells are among the major mechanisms tumor immunoevasion. However, expression pattern these subpopulations a different activation status at sites is poorly characterized. Thus, we analyzed PD-1 CTLA-4 naïve, activated, memory, activated memory cells. Bronchoalveolar lavage fluid (BALF) from lung affected by cancer (clBALF), opposite ‘healthy’ (hlBALF), peripheral blood (PB) samples were...

Cell response to novel coronavirus disease 19 (COVID-19) is currently a widely researched topic. The assessment of leukocytes population and the maturation both B T lymphocytes may be important in characterizing immunological profile COVID-19 patients. aim present study was evaluate cells patients with interstitial lesions on chest X-ray (COVID-19 (+)), without changes (−)) healthy control. group consisted 23 divided two groups: (+) n = 14 (−) 9 control 20. flow cytometry method performed....

Identification of patients with activation the immune system which indicates presence infection is essential, especially in times global coronavirus 2019 (COVID-19) pandemic. The aim present study was to evaluate reactive lymphocytes (RE-LYMP) parameter COVID-19 and correlate it markers by flow cytometry. group consisted 40 patients: (n = 20) others virus infections without (COVID-19(−) 20)) 20 healthy donors (HC). Blood count cytometry were performed. COVID-19(+) had significantly lower...

The role of the adaptive microenvironment components in severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) infection is widely researched, but remains unclear. Studying common dynamics immune response changes can help understand pathogenesis disease 2019 (COVID-19), especially critical patients. aim present study was to determine cytokines concentration and leukocyte subpopulations profiles COVID-19 (n = 23) 18) group distinguished by computed tomography (CT) severity score. We...

Myelodysplastic syndromes (MDS) are common malignant disorders with a poor prognosis. It is necessary to search for new rapid diagnostic methods detect MDS patients cytogenetic changes. The aim of the study was assess hematological neutrophil- and monocyte- related parameters I then bone marrow patient without A total 45 MDS, including 17 changes, were examined. conducted using Sysmex XN-Series analyzer. New neutrophil monocyte parameters, such as immature granulocytes (IG), reactivity...

Most patients with primary immune deficiency suffer from recurrent infections; however, paradoxical autoimmune phenomena can also manifest. The aim of this study was to identify immunological markers associated common variable immunodeficiency (CVID). included 33 adults CVID divided into two groups: (1) those noninfectious complications (CVID-C (

The mechanisms underlying the immune response to coronavirus disease 2019 (COVID-19) and recovery process have not been fully elucidated. aim of study was analyze leukocyte subpopulations in patients at significant time points (at diagnosis, 3 6 months after infection) selected according analysis changes lungs by CT classification system, considering severity disease. groups consisted severe non-severe COVID-19 patients. There a decrease CD8+ T cells, NK eosinophils, with an increasing...

Objective: Local cytokine milieu (especially Th2 inflammatory type) secreted into the asthmatic airways affect alternative activated macrophages polarization (M2). TSLP and IL-33 are important alarmins of allergic response associated with inflammation. The aim study was to investigate expression receptors for epithelial derived cytokines: (TSLPR) (ST2) on induced sputum CD206 positive from asthma healthy subjects analyze relationships between these clinical features disease. Methods:...

A balance between tumor invasion and immune defence system is widely investigated. Objective . The aim of this study was to evaluate lymphocyte phenotype in lymph nodes (LNs) patients with lung cancer relation the presence metastases. Methods We investigated 364 LNs resected by transcervical extended mediastinal lymphadenectomy (TEMLA) 49 squamous cell carcinoma (SCC) or adenocarcinoma (AD) (A) without metastases (B). Expression CD4, CD8, CD25, CTLA-4, Foxp3 assessed immunohistochemical...

The efficacy of immune checkpoint inhibitors (ICIs) remains unexpected and in some patients the resistance to anti-programmed death-1 (anti-PD-1) death ligand 1 (anti-PD-L1) agents is observed. One possible explanation may be PD-L2 activity. PD-1 ligands: PD-L1 are present on cancer cells but also, not without significance, alveolar macrophages (AMs) contributing immune-suppression tumor microenvironment. aim this study was analyse PD-L2, expression AMs bronchoalveolar lavage fluid (BALF)...

Although the role of T lymphocytes in sarcoidosis (SA) and lung cancer (LC) is quite well reported, occurrence B cells disease microenvironments may suggest their potential as natural modifiers immune response. The aim this study was to investigate B-cell profile lymphocyte-related hematological parameters between patients with SA, LC healthy controls (HCs). were assessed by flow cytometry a analyzer peripheral blood (PB) material from lymph nodes (LNs) obtained EBUS/TBNA method. We showed...

The neutrophils evaluation seems interesting in the initial qualifications of patients with various inflammatory processes. In this study, we presented analysis and new parameters complexity (NEUT-GI, NE-WX), maturation (IG), size (NE-FSC, NE-WZ), neutrophil activities (NEUT-RI, NE-WY) coronavirus disease 2019 (COVID-19), lung cancer (LC), sarcoidosis (SA), healthy controls (HCs). Peripheral blood (PB) was collected. were examined by Sysmex XN-1500. mean absolute value for IG parameter...

Macrophages play an important role in the suppression and activation of immune anti-cancer response, but little is known about dominant macrophage phenotype lung cancer environment, evaluated by bronchoalveolar lavage fluid (BALF). The aim this study was to characterize macrophages BALF from a affected (cBALF) healthy (hBALF) same patient regarding their individual polarization selected cytokines profile. A total 36 patients with confirmed were investigated. markers: CD206 CD163 CD80 CD86...

Over the past decade, immune checkpoint inhibitors have revolutionized treatment of non-small cell lung cancer (NSCLC). Unfortunately, not all patients benefit from PD-(L)1 blockade, yet, PD-L1 tumor expression is only approved biomarker, and other biomarkers been investigated. In present study, we analyzed presence immunomodulatory molecules: PD-L1, CD47, CD73, Fas, FasL on mature cells (MTCs) stem (CSCs) in lymph nodes (LNs) aspirates refer it to lymphocyte subpopulation peripheral blood...