A5067230838- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Water Quality Monitoring and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- RNA and protein synthesis mechanisms
- Higher Education and Teaching Methods
- Polymer composites and self-healing
- Receptor Mechanisms and Signaling
- Polymer Nanocomposites and Properties
- Gas Sensing Nanomaterials and Sensors
- Constructed Wetlands for Wastewater Treatment
- Flame retardant materials and properties
- Metabolomics and Mass Spectrometry Studies
- Water Quality and Pollution Assessment
- Metal Forming Simulation Techniques
- Polymer Surface Interaction Studies
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Force Microscopy Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Advanced Welding Techniques Analysis
Thermo Fisher Scientific (United States)
2023-2025
Sichuan University
2024-2025
Jiangsu Provincial Academy of Environmental Science
2015-2024
Jiangsu University
2021-2024
Beijing University of Chinese Medicine
2019-2024
Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine
2019-2024
Massachusetts Institute of Technology
2023
University of Alberta
2023
Heilongjiang Academy of Sciences
2021
Pacific Northwest National Laboratory
2019
To control the spread of 2019 novel coronavirus (COVID-19), China imposed nationwide restrictions on movement its population (lockdown) after Chinese New Year 2020, leading to large reductions in economic activities and associated emissions. Despite such decreases primary pollution, there were nonetheless several periods heavy haze pollution eastern China, raising questions about well-established relationship between human air quality. Here, using comprehensive measurements modeling, we show...
One challenge associated with the discovery and development of monoclonal antibody (mAb) therapeutics is determination heavy chain light pairing. Advances in MS instrumentation MS/MS methods have greatly enhanced capabilities for analysis large intact proteins yielding much more detailed accurate proteoform characterization. Consequently, direct interrogation antibodies or F(ab′)2 Fab fragments has potential to significantly streamline therapeutic mAb processes. Here, we demonstrate first...
Adeno-associated viruses (AAVs) are non-enveloped ssDNA icosahedral T = 1 used as vectors for clinical gene delivery. Currently, there over 200 AAV-related trials and six approved biologics on the market. As such new analytical methods continually being developed to characterize monitor quality purity of manufactured AAV vectors, these include ion-exchange chromatography Direct Mass Technology. However, require homogeneous standards with a high molecular weight standard comparable mass an...
Monoclonal antibodies (mAbs) and antibody–drug conjugates (ADCs) are two of the most important therapeutic drug classes that require extensive characterization, whereas their large size structural complexity make them challenging to characterize demand use advanced analytical methods. Top-down mass spectrometry (TD-MS) is an emerging technique minimizes sample preparation preserves endogenous post-translational modifications (PTMs); however, TD-MS proteins suffers from low fragmentation...
The extent to which noncovalent protein complexes retain native structure in the gas phase is highly dependent on experimental conditions. Energetic collisions with background can cause structural changes ranging from unfolding subunit dissociation. Additionally, recent studies have highlighted role of charge such changes, but mechanism not completely understood. In this study, top down (native TD) mass spectrometry was used probe gas-phase alcohol dehydrogenase (ADH, 4mer) under varying...
BamA is the central component of essential β-barrel assembly machine (BAM), a conserved multi-subunit complex that dynamically inserts and folds proteins into outer membrane Gram-negative bacteria. Despite recent advances in our mechanistic structural understanding BamA, there are few potent selective tool molecules can bind to modulate activity. Here, we explored vitro selection methods different BamA/BAM protein formulations discover peptide macrocycles kill Escherichia coli by targeting...
Membrane proteins represent the majority of clinical drug targets and are actively involved in a range cellular processes. However, complexity membrane mimetics for protein solubilization poses challenges native mass spectrometry (MS) analyses. The most common approach MS analyses remains offline buffer exchange into MS-compatible buffers prior to manual sample loading static nano-ESI emitters. This laborious process requires relatively high consumption optimization individual proteins....
Ferritin degradation pathways, particularly NCOA4-mediated ferritinophagy, are crucial for maintaining iron homeostasis. Here, we demonstrate the coexistence of two NCOA4 isoforms, one iron-sulfur cluster-free and cluster-bound, in oxygenated cell cultures. Using a combination spectroscopic analytical techniques, vitro characterization fragment (383-522), denoted NCOA4-D, revealed predominance monomeric species with relatively stable [2Fe-2S] cluster under normoxic conditions. The results...
Rapid, convenient methods for monoclonal antibody (mAb) isolation are critical determining the concentrations of therapeutic mAbs in human serum. This work uses porous nylon membranes modified with a HER2 peptide mimotope, KGSGSGSQLGPYELWELSH (KH19), rapid affinity capture Herceptin, mAb used to treat breast cancer. Covalent linking KH19 poly(acrylic acid)-containing films leads Herceptin-binding capacity 10 mg per mL membrane and allows selective Herceptin from diluted (1:3) serum 5 min....
This paper describes a convenient synthesis of nitrilotriacetate (NTA)-containing polymers and subsequent layer-by-layer adsorption these on flat surfaces in membrane pores. The resulting films form NTA-metal-ion complexes capture 2-3 mmol metal ions per mL film. Moreover, coatings bind multilayers polyhistidine-tagged proteins through association with complexes. Inclusion acrylic acid repeat units NTA-containing copolymers promotes swelling to increase protein binding Au-coated wafers....
Trypsin-containing membranes effect limited digestion to identify facile sites in protein structures.
Bacterial cell surface glycoconjugates are critical for survival and interactions between bacteria their hosts. Consequently, the pathways responsible biosynthesis have untapped potential as therapeutic targets. The localization of many glycoconjugate enzymes to membrane represents a significant challenge expressing, purifying, characterizing these enzymes. Here, we leverage cutting-edge detergent-free methods stabilize, purify, structurally characterize WbaP, phosphoglycosyl transferase...