Long noncoding RNAs (lncRNAs) are essential regulators of gene expression in eukaryotes. Despite increasing knowledge on the function lncRNAs, little is known about their effects crop yield. Here, we identify a lncRNA transcribed from antisense strand neighbouring LRK (leucine-rich repeat receptor kinase) cluster named LAIR (LRK Antisense Intergenic RNA). overexpression increases rice grain yield and upregulates several genes. Additionally, chromatin immunoprecipitation assay results...

Fluorescent carbon dots (CDs) have been widely studied in bioscience and bioimaging, but the effect of CDs on plants has rarely studied. Herein, mung bean was adopted as a model plant to study phytotoxicity, uptake, translocation red emissive plants. The incubation with at concentration range from 0.1 1.0 mg/mL induced physiological response imposed no phytotoxicity growth. lengths root stem presented an increasing trend up treatment 0.4 mg/mL. Confocal imaging showed that were transferred...

Abstract Light and nutrient are important factors for vegetable production in plant factory or greenhouse. The total 12 treatments which contained the combination of four light intensity (150, 250, 350 450 μmol · m −2 s −1 ) three solution concentration (NSC) (1/4, 1/2, 3/4 strength NSC) were established investigation lettuce growth quality a chamber. NSC exhibited significant effects on photosynthetic pigment, nutritional quality, mineral content antioxidant capacity. That higher readily...

This study investigated the individual and combined effects of ultraviolet A (UV-A) far-red (FR) light irradiation on biomass, phytochemical accumulation, antioxidant capacity two lettuce cultivars in an artificial plant factory. UV-A supplementation yielded a smaller leaf area reduced biomass nitrate content. In contrast, it improved chlorophyll, soluble protein, sugar, vitamin C, flavonoid, polyphenol, anthocyanin contents 2,2-diphenyl-1-picrylhydrazyl radical-scavenging rate. FR resulted...

Mammalian calreticulin (CRT) is a multifunctional Ca(2+)-binding protein involved in more than 40 cellular processes various subcellular compartments, such as Ca(2+) storage and folding the endoplasmic reticulum (ER). CRT homologues were discovered plants almost 15 years ago, recent studies revealed that many plant species contain 2 or CRTs are members of distinct families, CRT1/2 family plant-specific CRT3 family. However, little known about their physiological functions. Here we report...

Endoplasmic reticulum (ER)-associated degradation (ERAD) is an integral part of the ER quality-control system that removes toxic misfolded proteins via ubiquitin/proteasome-mediated degradation. Most our knowledge on ERAD comes from biochemical and genetic studies in yeast mammalian cells. Although known to operate plant cells, little about its molecular components mechanism. A screen for suppressors Arabidopsis bri1-9 , a weak dwarf mutant caused by retention structurally defective yet...

Lycopene, one of the strongest natural antioxidants known and main carotene in ripe tomato, is very important for human health. Light well to be most environmental stimuli influencing lycopene biosynthesis; specifically, red light induces higher content tomato. However, whether blue promotes synthesis remains elusive exactly how stimulation unclear. We applied supplemental lighting on tomato plants at anthesis monitor effect synthesis. Our results showed that blue/red induced fruits;...

To produce high-quality broccoli microgreens, suitable light intensity for growth and phytochemical contents of microgreens in an artificial plant factory were studied. Broccoli irradiated under different photosynthetic photon flux density (PPFD): 30, 50, 70 90 μmol·m−2·s−1 with red: green: blue = 1:1:1 light-emitting diodes (LEDs). The grown 50 had the highest fresh weight, dry moisture content, while lowest. With increasing intensity, chlorophyll content increased, whereas carotenoid...

Abstract Plant growth and development are tightly regulated by phytohormones. However, little is known about the interaction between auxin gibberellin acid (GA) during flower stalk elongation how it directly related to organ formation. Therefore, effects of indole acetic (IAA) GA 3 treatments their on in flowering Chinese cabbage were investigated. The IAA 3, opposite results observed after with uniconazole (GA synthesis inhibitor) N-1-naphthylphthalamic (NPA) (auxin transport inhibitor)....

Flowering Chinese cabbage is one of the most economically important stalk vegetables. However, molecular mechanisms underlying bolting, which directly related to quality and yield, in this species remain unknown. Previously, we examined five key stem development stages flowering cabbage. Here, identified a gene, BcSOC1 (SUPPRESSOR OF OVEREXPRESSION CONSTANS1), using transcriptome analysis, whose expression was positively correlated with bolting. Exogenous gibberellin (GA3) low-temperature...

Nitrate reductase (NR) is rapidly inactivated by phosphorylation of serine residues in response to loss light or reduction CO2 levels. To identify sites within NR protein that play a role this post-translational regulation, heterologous expression system and an vitro inactivation assay for Arabidopsis were developed. Protein extracts containing kinases inhibitor proteins prepared from NR-defective mutant had lesions both the NIA1 NIA2 genes Arabidopsis. Active was produced Pichia pastoris...

Significance Endoplasmic reticulum (ER)-associated degradation (ERAD) is a well-studied cellular process in yeast and mammalian systems. Recent molecular genetic studies the reference plant Arabidopsis have revealed that ERAD also conserved plants. Here we report an for degrading misfolded/mutant receptor-like kinases requires plant-specific protein, ethyl methanesulfonate-mutagenized brassinosteroid-insensitive 1 suppressor 7 (EBS7), localized to ER membrane induced by stress. Our...

In greenhouse tomato production, the lower canopy of plants is often shaded by neighbouring plants. Supplementary lighting can be used to provide sufficient light energy for Red regarded as most efficient spectrum in supplementary lighting. Whether and how red ambient sunlight affect ripening remain elucidated. this research, impacts on were examined comparing ripening-related regulators, ethylene synthesis, signaling, carotenoid synthesis signaling Micro-Tom wild type fruit...

With far-red-light supplementation (3 W·m-2, and 6 W·m-2), the flower budding rate, plant height, internode length, display, stem diameter of Chinese kale were largely elevated, as well leaf morphology such width, petiole area. Consequently, fresh weight dry edible parts markedly increased. The photosynthetic traits enhanced, mineral elements accumulated. To further explore mechanism that far-red light simultaneously promoted vegetative growth reproductive kale, this study used RNA...

Gibberellin (GA) plays a major role in controlling Brassica rapa stalk development. As an essential negative regulator of GA signal transduction, DELLA proteins may exert significant effects on However, the regulatory mechanisms underlying this regulation remain unclear. In study, we report highly efficient and inheritable mutagenesis using CRISPR/Cas9 gene editing system BraPDS (phytoene desaturase) BraRGL1 (key protein) genes. We observed loss-of-function mutation due to two amino acids...

Asparagine-linked glycans (N-glycans) are crucial signals for protein folding, quality control, and endoplasmic reticulum (ER)-associated degradation (ERAD) in yeast mammals. Although similar ERAD processes were reported plants, little is known about their biochemical mechanisms, especially relationships with N-glycans. Here, we show that a missense mutation the Arabidopsis EMS-mutagenized bri1 suppressor 3 ( EBS3 ) gene suppresses dwarf mutant, bri1-9, phenotypes of which caused by ER...

Three different LED spectra (W: White light; WFR: W + far-red WB: blue light) with similar photosynthetic photon flux density (PPFD) were designed to explore the effects of supplementary and lights on leaf color, biomass phytochemicals two cultivars red-leaf lettuce ("Yanzhi" "Red Butter") in an artificial lighting plant factory. Lettuce plants under WB had redder color significantly higher contents pigments, such as chlorophyll a, b, (a b) anthocyanins. The accumulation health-promoting...