A5049387289- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Photoacoustic and Ultrasonic Imaging
- Lipid Membrane Structure and Behavior
- Photodynamic Therapy Research Studies
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- CAR-T cell therapy research
- Angiogenesis and VEGF in Cancer
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Porphyrin and Phthalocyanine Chemistry
- biodegradable polymer synthesis and properties
- Metabolomics and Mass Spectrometry Studies
- Advanced Fluorescence Microscopy Techniques
- MicroRNA in disease regulation
- Click Chemistry and Applications
- T-cell and B-cell Immunology
- Thermography and Photoacoustic Techniques
- Medical Imaging Techniques and Applications
- Infrared Thermography in Medicine
- Characterization and Applications of Magnetic Nanoparticles
National Cancer Institute
2023-2024
National Institutes of Health
2020-2024
Center for Cancer Research
2023-2024
National Institute of Biomedical Imaging and Bioengineering
2020
Wuhan National Laboratory for Optoelectronics
2011-2019
Huazhong University of Science and Technology
2009-2019
University Health Network
2011-2016
Princess Margaret Cancer Centre
2014-2016
University of Toronto
2011-2013
Ontario Institute for Cancer Research
2011-2013
PEGylation (PEG) is the most commonly adopted strategy to prolong nanoparticles' vascular circulation by mitigating reticuloendothelial system uptake. However, there remain many concerns in regards its immunogenicity, targeting efficiency, etc., which inspires pursuit of alternate, non-PEGylated systems. We introduced here a PEG-free, porphyrin-based ultrasmall nanostructure mimicking nature lipoproteins, termed PLP, that integrates multiple imaging and therapeutic functionalities, including...
High-density lipoprotein (HDL)-mimicking peptide–phospholipid scaffold (HPPS) nanoparticles are used as a flexible and biocompatible nanocarrier for the delivery of small interfering RNA (siRNA). The direct cytosolic ability cholesterol-modified siRNA displayed by nanoparticle results in selective interference (RNAi) to targeted cells. This provides strategy bypass endolysosomal trafficking, makes HPPS useful tool therapeutic delivery. Detailed facts importance specialist readers published...
Nanotheranostics based on tumor-selective small molecular prodrugs could be more advantageous in clinical translation for cancer treatment, given its defined chemical structure, high drug loading efficiency, controlled release, and reduced side effects. To this end, we have designed synthesized a reactive oxygen species (ROS)-activatable heterodimeric prodrug, namely, HRC, nanoformulated it imaging synergistic chemo- photodynamic therapy. The prodrug consists of the chemodrug camptothecin...
The abilities to deliver siRNA its intended action site and assess the delivery efficiency are challenges for current RNAi therapy, where effective will join force with patient genetic profiling achieve optimal treatment outcome. Imaging could become a critical enabler maximize efficacy in context of tracking delivery, rational dosimetry planning. Several imaging modalities have been used visualize nanoparticle‐based but rarely did they guide We report multimodal theranostic...
Although small interfering RNA (siRNA) can silence the expression of disease-related genes, delivery these highly charged molecules is challenging. Delivery approaches for siRNAs are actively being pursued, and improved strategies required nontoxic efficient gene knockdown. Low density lipoprotein (LDL) a natural endogenous nanoparticle that has rich history as vehicle. Here, we examine purified LDL nanoparticles carriers siRNAs. When siRNA was covalently conjugated to cholesterol, over 25...
The main challenge for RNAi therapeutics lies in systemic delivery of siRNA to the correct tissues and transporting them into cytoplasm targeted cells, at safe, therapeutic levels. Recently, we developed a high-density lipoprotein-mimicking peptide-phospholipid scaffold (HPPS) demonstrated its direct cytosolic vitro, thereby bypassing endosomal trapping.We investigate vivo implementation HPPS delivery.After administration KB tumor-bearing mice, prolonged blood circulation time...
Kupffer cells (KCs), potent scavenger located in hepatic sinusoids, constantly phagocytize and degrade foreign materials to maintain metabolism clearance. Understanding the strategic KC arrangement which links their spatial location function lobules, basic functional unit liver, is highly valuable for characterizing liver function. However, selectively labeling KCs vivo remains challenging. Herein, a fast self-assembled pomegranate structure-like nanoparticle with "nanopomegranate seeds" of...
In adoptive T cell therapy (ACT), the direct cytotoxic effects of CD8 cells on tumor cells, including release interferon-gamma (IFN-γ), are considered primary mechanism for eradication. Cancer antigen escape diminishes responses, thereby limiting therapeutic success. The impacts IFN-γ targeting non-tumor in ACT, other hand, remains under-investigated. We hypothesized that action particularly vascular endothelial within physiological microenvironment, could influence efficacy.
The development of experimental animal models for head and neck tumors generally rely on the bioluminescence imaging to achieve dynamic monitoring tumor growth metastasis due complicated anatomical structures. Since is largely affected by intracellular luciferase expression level external D-luciferin concentrations, its accuracy requires further confirmation. Here, a new triple fusion reporter gene, which consists herpes simplex virus type 1 thymidine kinase (TK) gene radioactive imaging,...
Breast cancer is one of the most harmful cancers in human. Its early diagnosis expected to improve patients' survival rate. X-ray computed tomography (CT) has been widely used tumor detection for obtaining three-dimentional information. Fluorescence Molecular Tomography (FMT) imaging combined with near-infrared fluorescent dyes provides a powerful tool acquisition molecular biodistribution information deep tissues. Thus, combination CT and FMT modalities allows us better differentiate...
Photodynamic therapy (PDT) gains wide attention as a useful therapeutic method for cancer. It is mediated by the oxygen and photosensitizer under specific light irradiation to produce reactive species (ROS), which induce cellular toxicity regulate redox potential in tumor cells. Nowadays, genetic photosensitizers of low easy production are required be developed. KillerRed, unique red fluorescent protein exhibiting excellent phototoxic properties, has act application PDT. Meantime, course...
Abstract Adoptive T cell therapy relies on trafficking of infused cells to the tumor. Chemokine gradients and chemokine receptors expressed mediate this recruitment cells. However, due high complexity tumor microenvironment, regulation homing tumors remains poorly understood. We examined how various impact their ability home cancer using zirconium-89 (89Zr)-oxine labeling. Ex vivo activated mouse cells, wild type (WT) or deficient (KO) for a receptor, were labeled with 89Zr-oxine...
Glioblastoma is the most lethal form of brain tumors and one fatal human cancers with universal recurrence post-surgical procedures common failure in salvage therapies. Fluorescence agents introduced to facilitate identification cancerous tissues surgical resection are facing challenges such as low sensitivity specificity ineffective delivery intracerebral tumor via systemic administration. Recently, we have developed a porphyrin-lipid assembled HDL-like nanovesicle (porphyHDL) sub 20 nm...
The cytosolic drug delivery mechanism is a key advantage for the of intracellular active cancer agents. Here, we use confocal microscopy to investigate HDL-mimicking peptide-phospholipid scaffold (HPPS).
Here, we developed a breast cancer-targeting nanoparticle for dual-modality imaging based on incorporation of CT contrast agents (iodinated oil) and far-infrared fluorescent dyes (DiR-BOA) into core nanoparticles, obtained FMT signals simultaneously.