A5053588782- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Molecular Biology Techniques and Applications
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Organ Transplantation Techniques and Outcomes
- Ethics in Clinical Research
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- Innovation and Socioeconomic Development
- Organ Donation and Transplantation
- Ethics and Legal Issues in Pediatric Healthcare
- Spinal Cord Injury Research
- RNA regulation and disease
- Neuroethics, Human Enhancement, Biomedical Innovations
- RNA modifications and cancer
- Transplantation: Methods and Outcomes
American Society of Law, Medicine and Ethics
2024
Mayo Clinic Hospital
2024
Mayo Clinic in Arizona
2024
Translational Genomics Research Institute
2017-2023
Cardiff University
2022
The Foundational Data Initiative for Parkinson Disease (FOUNDIN-PD) is an international collaboration producing fundamental resources disease (PD). FOUNDIN-PD generated a multi-layered molecular dataset in cohort of induced pluripotent stem cell (iPSC) lines differentiated to dopaminergic (DA) neurons, major affected type PD. were derived from the Parkinson's Progression Markers study, which included participants with PD carrying monogenic variants, variants intermediate effects, and...
Therapeutic development for spinal cord injury is hindered by the difficulty in conducting clinical trials, which to date have relied solely on functional outcome measures patient enrollment, stratification, and evaluation. Biological biomarkers that accurately classify severity predict neurologic would represent a paradigm shift way trials could be conducted. MicroRNAs emerged as attractive biomarker candidates due their stability biological fluids, phylogenetic similarities, tissue...
Exercise improves cardiometabolic and vascular function, although the mechanisms remain unclear. Our objective was to demonstrate diversity of circulating extracellular RNA (ex-RNA) release during acute exercise in humans its relevance exercise-mediated benefits on inflammation. We performed plasma small sequencing 26 individuals undergoing symptom-limited maximal treadmill exercise, with replication our top candidate miRNA a separate cohort 59 bicycle ergometry. found changes miRNAs other...
Normothermic Regional Perfusion, or NRP, is a method of donated organ reperfusion using cardiopulmonary bypass modified extracorporeal membrane oxygenation (ECMO) circuit after circulatory death while leaving organs in the dead donor's corpse. Despite its potential, several key ethical issues remain unaddressed by this technology.
One promising goal for utilizing the molecular information circulating in biofluids is discovery of clinically useful biomarkers. Extracellular RNAs (exRNAs) are one most diverse classes cargo, easily assayed by sequencing and with expressions that rapidly change response to subject status. Despite exRNA evaluations from have focused on small RNA analysis, specifically microRNAs (miRNAs). Another characterizing information, correlate expression injuries associated specific tissues origin....
Alzheimer’s disease (AD), due to its multifactorial nature and complex etiology, poses challenges for research, diagnosis, treatment, impacts millions worldwide. To address the need minimally invasive, repeatable measures that aid in AD diagnosis progression monitoring, studies leveraging RNAs associated with extracellular vesicles (EVs) human biofluids have revealed AD-associated changes. However, validation of biomarkers has suffered from collection samples differing points time course or...
The potential utility of microRNA as biomarkers for early detection cancer and other diseases is being investigated with genome-scale profiling differentially expressed microRNA. Processes measurement assurance are critical components measurements. Here, we evaluated the a set total RNA samples, designed between-sample differences in relative abundance miRNAs, process controls. Three pure human samples (brain, liver, placenta) two different mixtures these were control on multiple systems at...
Abstract The FOUNdational Data INitiative for Parkinson’s Disease (FOUNDIN-PD) is an international collaboration producing fundamental resources disease (PD). FOUNDIN-PD generated a multi-layered molecular dataset in cohort of induced pluripotent stem cell (iPSC) lines differentiated to dopaminergic (DA) neurons, major affected type PD. were derived from the Progression Markers Initiative study including participants with PD carrying monogenic ( SNCA ) variants, variants intermediate effects...
The development of the CRISPR gene editing technique has been hyped as a that could fundamentally change scientific research and its clinical application. Unrecognized is fact it joins other technologies have tried failed under same discourse hype. These technologies, like therapy stem cell research, moved quickly passed basic into application with dire consequences. Before hastily moving to applications, necessary consider determine how CRISPR/Cas systems should be applied. In case single...
ABSTRACT Background The potential utility of microRNA as biomarkers for early detection cancer and other diseases is being investigated with genome-scale profiling differentially expressed microRNA. Processes measurement assurance are critical components measurements. Here, we evaluated the a set total RNA samples, designed between-sample differences in relative abundance miRNAs, process controls. Results Three pure human samples (brain, liver, placenta) two different mixtures these were...
The benefits of acute exercise on distant organs (including the neurocognitive system) have been recognized, suggesting existence biomarkers that mediate in target organs. Extracellular RNAs (ex-RNAs) are RNA molecules stable circulation, capable regulating gene expression across multiple at a distance. In 26 individuals without clinical cardiovascular disease (myocardial infarction or heart failure), we performed small non-coding sequencing (RNA-seq) from plasma before and after...