A5059469952- Parasite Biology and Host Interactions
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Cytokine Signaling Pathways and Interactions
- Erythropoietin and Anemia Treatment
- Primate Behavior and Ecology
- Animal Behavior and Reproduction
- Erythrocyte Function and Pathophysiology
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Outbreaks Research
Center for Systems Biology
2019-2024
Harvard University
2024
Colorado College
2017
The ability to rapidly assemble and prototype cellular circuits is vital for biological research its applications in biotechnology medicine. Current methods the assembly of mammalian DNA are laborious, slow, expensive. Here we present Mammalian ToolKit (MTK), a Golden Gate-based cloning toolkit fast, reproducible, versatile large vectors their implementation models. MTK consists curated library characterized, modular parts that can be assembled into transcriptional units further weaved...
Feedback control of erythropoiesis exemplifies conflicting goals in tissue homeostasis: maintaining fast reactivity to stress while minimizing proliferative burden on progenitors the steady state. Here we show that these are tuned through combinatorial action cytokines. We find IL-17A, a pro-inflammatory cytokine, mediates striking synergism with negative feedback signal erythropoietin (Epo) vivo, accelerating erythropoietic response hypoxia. A model shows increased may occur two cell...
Abstract The ability to rapidly assemble and prototype cellular circuits is vital for biological research its applications in biotechnology medicine. Current methods that permit the assembly of DNA mammalian cells are laborious, slow, expensive mostly not permissive rapid prototyping constructs. Here we present Mammalian ToolKit (MTK), a Golden Gate-based cloning toolkit fast, reproducible versatile large vectors their implementation models. MTK consists curated library characterized,...