A5026844996- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Chronic Lymphocytic Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Immunodeficiency and Autoimmune Disorders
- Inflammasome and immune disorders
- Autoimmune and Inflammatory Disorders Research
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Mesenchymal stem cell research
- Lymphoma Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- Dermatology and Skin Diseases
- Hematological disorders and diagnostics
- Cutaneous lymphoproliferative disorders research
- Systemic Lupus Erythematosus Research
- Platelet Disorders and Treatments
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Blood groups and transfusion
- Blood disorders and treatments
- Monoclonal and Polyclonal Antibodies Research
Istituti di Ricovero e Cura a Carattere Scientifico
2005-2024
Istituto Giannina Gaslini
2009-2024
Brescia University
2020
University of Genoa
1997-2015
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2015
Ospedale Policlinico San Martino
2005
Mario Negri Institute for Pharmacological Research
2000
Martin University
2000
Ospedali Riuniti di Ancona
1997
Alleanza Contro il Cancro
1988-1992
Clonally expanded populations of B cells carrying somatic mutations Ig variable (V) region genes have been detected in the CNS subjects with multiple sclerosis (MS), suggesting that a process cell affinity maturation ensuing production potentially pathogenic autoantibodies may occur inside CNS. Here, we characterized subsets present cerebrospinal fluid (CSF) MS patients and individuals other inflammatory neurological disorders by flow cytometry. CD19 + CD38 high+ CD77 , Ki67 Bcl-2 –...
Abstract IL-12 activates murine and human B cells, but little information is available as to the expression function of IL-12R on lymphocytes. Here we show that latter freshly isolated from tonsils, expressed transcripts both β1 β2 chains chain mRNA was selectively increased (4- 5-fold) by incubation with Staphylococcus aureus Cowan I bacteria or IL-12. cell stimulation induced de novo two IL-18R, i.e., IL-1 receptor-related protein accessory protein-like. Functional studies showed IL-18...
Background: Follicular center lymphoma displays widespread lymph node involvement at diagnosis. The chemoattractants that control the locomotion of follicular B cells have not been established. Stromal cell-derived factor-1 (SDF-1) is a CXC-class chemokine enhances migration normal human and expressed in peripheral lymphoid tissues. Here we investigated 1) whether SDF-1 stimulates vitro their presumed counterparts (i.e., germinal cells) 2) same express transcripts. Methods: were purified by...
Systemic juvenile idiopathic arthritis (sJIA), a multifaceted autoinflammatory disorder, can be complicated by life-threatening conditions such as macrophage activation syndrome (MAS) and interstitial lung disease (ILD). The management of these presents therapeutic challenge, underscoring the need for innovative treatment approaches.
Twelve paediatric patients with aplastic anaemia and two groups of normal control subjects underwent flow cytometric analysis for intracytoplasmic expression γ interferon (γ‐IFN) tumour necrosis factor α (TNF‐α) in bone marrow peripheral blood CD4+ CD8+ cells. The same cytokines were tested, by immunoassay, culture supernatants from unstimulated mononuclear cells (MNCs). Marrow expressing γ‐IFN TNF‐α significantly increased the comparison ( P < 0·05 to 0·0001 different cellular subsets)....
Human C5a is a potent chemoattractant for granulocytes, monocytes, and dendritic cells. In mice has been shown to be chemotactic germinal center (GC) B To date, no information available on the effects of human cell locomotion. Here we demonstrate that rC5a increases polarization migration tonsillar The locomotory response was due both chemokinetic activities rC5a. Moreover, memory and, at lesser extent, naive fractions from purified populations displayed rC5a-enhanced migratory properties,...
Abstract Purpose: Few data are available in the literature on chemokine receptor expression and migratory capability of mantle cell lymphoma (MCL) B cells. Information these issues may allow us to identify novel mechanisms chemokine-driven tumor migration. Experimental Design: The research was designed investigate: (a) CCR1 CCR7 CXCR1 CXCR5 receptors; (b) chemotaxis respective ligands MCL cells their normal counterparts, i.e., CD5+ Results: Malignant from patients counterparts displayed...
Background and Objectives Congenital amegakaryocytic thrombocytopenia (CAMT) is a rare, autosomal recessive disorder induced by mutations of the gene coding for thrombopoietin (TPO) receptor (c-MPL). Patients initially present with isolated that subsequently progresses into pancytopenia. Although mechanisms leading to aplasia are unknown, age onset has been reported depend on severity c-MPL functional defect. To improve our knowledge in this field, we studied clinical biological features...
In chronic inflammatory disorders, B cells can contribute to tissue damage by autoantibody production and antigen presentation T cells. Here, we have characterized synovial fluid B-cell subsets in patients with oligoarticular juvenile idiopathic arthritis (JIA), an issue not addressed before detail. from (SF) peripheral blood (PB) of 25 JIA patients, as well PB 20 controls comparable age, were multicolor flow cytometry. Immunoglobulin-secreting detected ELISPOT. Immunohistochemical analyses...
Background Fractalkine/CX3CL1, a surface chemokine, binds to CX3CR1 expressed by different lymphocyte subsets. Since CX3CL1 has been detected in the germinal centres of secondary lymphoid tissue, this study we have investigated expression and function human naïve, centre memory B cells isolated from tonsil or peripheral blood. Methodology/Principal Findings We demonstrate unambiguously that highly purified blood at mRNA protein levels as assessed quantitative PCR, flow cytometry competition...
C-X3-C motif chemokine ligand 1 (CX3CL1)/fractalkine is a released after cleavage by two metalloproteases, ADAM metallopeptidase domain 10 (ADAM10) and 17 (ADAM17), involved in inflammation angiogenesis the cancer microenvironment. The role of CX3CL1/ receptor 1(CX3CR1) axis multiple myeloma (MM) microenvironment still unknown. Firstly, we analyzed bone marrow (BM) plasma levels CX3CL1 111 patients with cell disorders including 70 active MM, 25 smoldering (SMM), 16 monoclonal gammopathy...
We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)‐1α/CCL3, MIP‐1β/CCL4, MIP‐3α/CCL20, MIP‐5/CCL15, monocyte chemotactic (MCP)‐1/CCL2, MCP‐2/CCL8, MCP‐3/CCL7, eotaxin/CCL11 and macrophage‐derived chemokine (MDC)/CCL22 on locomotion human tonsil B lymphocytes their subsets. Upon isolation, cells were poorly responsive, but, following short‐term culture, they displayed statistically significant responses (P < 0.001) to MIP‐1α, MIP‐5, MCP‐1,...
AC133+ cells may represent an alternative source of transplantable haemopoietic progenitor to CD34+ cells. Here, we have addressed the characterization umbilical cord blood (UCB) and compared their immunophenotypic functional features with those UCB cell fractions were purified by magnetic sorting, analysed flow cytometry, tested for content in blast colony-forming units (CFU-Bl), erythroid granulocyte-macrophage before after expansion presence various growth factor combinations. Median...
Cytokine expression assessed by flow cytometry in 53 acquired aplastic anemia patients before and after combined immunosuppression (EBMT WPSAA protocols) showed that CD3(+) marrow cells containing TNF-alpha, IFN-gamma IL4 were similar subjects with disease at onset (DO) responsive to treatment who had more CD3(+)/TNF-alpha(+) CD 3(+)/IFN-gamma(+) than normal controls. In vitro block of TNF-alpha and/or significantly increased BFU-e over baseline 28 patients. only incremented colonies...