A5074085320- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Insect Resistance and Genetics
- RNA modifications and cancer
- Lung Cancer Research Studies
- Peptidase Inhibition and Analysis
- Chronic Lymphocytic Leukemia Research
- Insect-Plant Interactions and Control
- Chronic Myeloid Leukemia Treatments
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Oral microbiology and periodontitis research
- Vaccine Coverage and Hesitancy
- Antimicrobial Peptides and Activities
- Protein Degradation and Inhibitors
- PARP inhibition in cancer therapy
- Vibrio bacteria research studies
- Health and Well-being Studies
- HER2/EGFR in Cancer Research
- Bacterial Genetics and Biotechnology
- Research on scale insects
- Bacteriophages and microbial interactions
- Synthesis and biological activity
- Enzyme Structure and Function
Christian Medical College
2003-2025
Universitäts-Herzzentrum Freiburg-Bad Krozingen
2025
University of Freiburg
2025
Cawthron Institute
2025
Chandigarh University
2024
Guru Gobind Singh Medical College and Hospital
2016-2024
Punjab Agricultural University
2010-2023
National Institutes of Health
1999-2023
National Center for Biotechnology Information
2017-2023
Thapar Institute of Engineering & Technology
2022-2023
2-(4-Aminophenyl)benzothiazoles 1 and their N-acetylated forms have been converted to C- N-hydroxylated derivatives investigate the role of metabolic oxidation in mode action this series compounds. 2-(4-Amino-3-methylphenyl)benzothiazole (1a, DF 203, NSC 674495) is a novel potent antitumor agent with selective growth inhibitory properties against human cancer cell lines. Very low IC50 values (<0.1 μM) were encountered most sensitive breast lines, MCF-7 T-47D, whereas renal line TK-10 was...
Background: Small cell lung carcinoma (SCLC) is an aggressive, recalcitrant cancer, often metastatic at diagnosis and unresponsive to chemotherapy upon recurrence, thus it challenging treat. Methods: Sixty-three human SCLC lines three NSCLC were screened for response 103 US Food Drug Administration–approved oncology agents 423 investigational agents. The library was a diverse set of small molecules that included multiple compounds targeting the same molecular entity. in triplicate nine...
The spotted bollworm Earias vittella (Lepidoptera: Nolidae) is a polyphagous pest with enormous economic significance, primarily affecting cotton and okra. However, the lack of gene sequence information on this has significant constraint molecular investigations formulation superior management strategies. An RNA-seq-based transcriptome study was conducted to alleviate such limitations, de novo assembly performed obtain transcript sequences pest. Reference identification across E....
Abstract Purpose: The purpose of this study was to investigate the antiangiogenic properties 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin (17-DMAG; NSC707545), a water-soluble benzoquinone ansamycin. Experimental Design: activity 17-DMAG, in vivo, evaluated for inhibition fibroblast growth factor (FGF)-2-induced angiogenesis s.c. implanted Matrigel mice. In vitro, 17-DMAG on endothelial cells (human umbilical vein cells; HUVEC) tested FGF-2; and vascular (VEGF)-induced proliferation...
Archaeosomes as liposomes made with one or more ether lipids that are unique to the domain of Archaeobacteria, found in Archaea constitute a novel family liposome. Achaean-type consist archaeol (diether) and/or caldarchaeol (tetraether) core structures. can be produced using standard procedures (hydrated film submitted sonication, extrusion and detergent dialysis) at any temperature physiological range lower, therefore making it possible encapsulate thermally stable compounds. Various well...
Adrenomedullin is a secreted peptide hormone with multiple activities. Several reports have indicated that adrenomedullin may be involved in tumor survival, but this has not been directly shown. Here we evaluate the vitro and vivo effects of overexpression human breast cancer cells.The cell lines T47D MCF7, both which express low basal levels adrenomedullin, were stably transfected an expression construct contained coding region gene or empty vector. Properties cells assessed by...
Amrasca biguttula (Ishida) commonly known as cotton leafhopper is a severe pest of and okra. Not much on this insect at molecular level due to lack genomic transcriptomic data. To prepare for functional studies in insect, we evaluated 15 common housekeeping genes (Tub, B-Tub, EF alpha, GADPH, UbiCF, RP13, Ubiq, G3PD, VATPase, Actin, 18s, 28s, TATA, ETF, SOD Cytolytic actin) during different developmental stages under starvation stress. We selected early (1st 2nd), late (3rd 4th) stage nymphs...
Social cellular aggregation or multicellular organization pose increased risk of transmission infections through the system upon infection a single cell. The generality evolutionary responses to this outside Metazoa remains unclear. We report discovery several thematically unified, remarkable biological conflict systems preponderantly present in prokaryotes. These combine thresholding mechanisms utilizing NTPase chaperones (the MoxR-vWA couple), GTPases and proteolytic cascades with...
We have examined a series of tyrosine kinase inhibitors structurally related to erbstatin (tyrphostins) for Inhibition p210bcr-abl autokinase activity in vitro and growth chronic myelogenous leukemia (CML) K562 cells. Of the tyrphostins with IC50 <50 μM, AG814, AG946, AG952, AG896, AG9S3, AG956 AG957 (structurally lavendustin A piceatannol) completely Inhibited an Immune complex assay. Another group (AG807, AG568, AG763, AG1076, AG490, AG1318, AG556, AG1319, AG5S5 AG1111) Inhibits cells but...
Small cell lung cancer (SCLC) is an extremely aggressive that frequently recurs. Twenty-three human SCLC lines were selected representing varied Myc status. Gene expression of cancer, stem-like, hedgehog pathway, and notch pathway genes determined by RT(2)-PCR array Exon 1.0 ST array. Etoposide topotecan concentration response was examined. The IC50's for etoposide ranged over nearly 3 logs upon 96 hrs exposure to the drugs. status, TOP2A, TOP2B TOP1 mRNA or topoisomerase 1 2 protein did not...
Methylation of histone H3 lysine 27 (H3K27) is widely recognized as a transcriptionally repressive chromatin modification but the mechanism repression remains unclear. We devised and implemented forward genetic scheme to identify factors required for H3K27 methylation-mediated silencing in filamentous fungus Neurospora crassa identified bromo-adjacent homology (BAH)-plant homeodomain (PHD)-containing protein, EPR-1 (effector polycomb 1; NCU07505). associates with H3K27-methylated chromatin,...
Several homologous domains are shared by eukaryotic immunity and programmed cell-death systems poorly understood bacterial proteins. Recent studies show these to be components of a network highly regulated connecting apoptotic processes counter-invader immunity, in prokaryotes with multicellular habit. However, the provenance key adaptor domains, namely those Death-like TRADD-N superfamilies, quintessential feature metazoan systems, remained murky. Here, we use sensitive sequence analysis...
Development of cancer therapeutics partially depends upon selection appropriate animal models. Therefore, improvements to model are beneficial. Forty-nine human tumor xenografts at in vivo passages 1, 4 and 10 were subjected cDNA microarray analysis yielding a dataset 823 Affymetrix HG-U133 Plus 2.0 arrays. To illustrate mining strategies supporting therapeutic studies, transcript expression was determined: 1) relative other models, 2) with successive passage, 3) during the vitro transition....
Thrips tabaci is a severe pest of onion and cotton. Due to lack information on its genome or transcriptome, not much known about this insect at the molecular level. To initiate studies in insect, RNA was sequenced; de novo transcriptome assembly analysis were performed. The RNAseq data used identify reference RNAi pathway genes insect. Additionally, feeding demonstrated T. for first time. From assembled 27,836 coding sequence (CDS) with an average size 1236 bp per CDS identified. About 85.4%...
Urinary Tract Infection (UTI) is a globally widespread human infection caused by an infestation of uropathogens. Eventhough, Escherichia coli often quoted as being the chief among them, Staphylococcus aureus involvement in UTI especially gestational understated. Staphylococcal accessory regulator A (SarA) quorum S. that controls expression various virulence and biofilm phenotypes. Since SarA had been focussed target for antibiofilm agent development, study aims to develop potential drug...
Dental caries occurs as a result of dysbiosis among commensal and pathogenic bacteria leading to demineralization enamel within dental biofilm (plaque) consequence lower pH in the oral cavity. In our previous study, we have reported 1,3-disubstituted ureas particularly, 1,3-di-m-tolylurea (DMTU) could inhibit formation along with concentrations fluoride (31.25 ppm) without affecting bacterial growth. present RT-qPCR analysis showed target specific molecular mechanism DMTU. vivo treatment...
Multi-drug resistant Staphylococcus aureus (MDRSA) remains a great challenge despite decade of research on antimicrobial compounds against their infections. In the present study, various acyclic amines and diamines were chemically synthesized tested for as well antibiofilm activity MDRSA. Among all compounds, an diamine, (2,2'-((Butane-1,4-diylbis(azanediyl)bis(methylene))diphenol) designated asADM 3, showed better (MIC at 50 μg/mL) (MBIC50 5 μg/mL). addition, ADM 3 was capable reducing...