Mitochondria are dynamic organelles that constantly change morphology. What controls mitochondrial morphology however remains unresolved. Using actively respiring yeast cells growing in distinct carbon sources, we find and activity unrelated. Cells can exhibit fragmented or networked different nutrient environments independent of activity. Instead, is controlled by the intracellular redox state, which itself depends on nature electron entry into transport chain (ETC)—through complex I/II...

Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing ∆ubp3 cells, orthogonal approaches,...

Cells contain disparate amounts of distinct amino acids, each which has different metabolic and chemical origins, but the supply cost vs demand requirements is unclear. Here, using yeast we quantify restoration-responses after disrupting acid supply, uncover a hierarchically prioritized restoration strategy for acids. We comprehensively calculate individual biosynthetic costs, total an acid, estimate cumulative supply/demand acid. Through this, discover that priority driven by gross itself...

Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing ∆ubp3 cells, orthogonal approaches,...

Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing Δubp3 cells, orthogonal approaches,...

Abstract Mitochondria are dynamic organelles that constantly change morphology. What controls mitochondrial morphology however remains unresolved. Using actively respiring yeast cells growing in distinct carbon sources, we find and activity unrelated. Cells can exhibit fragmented or networked different nutrient environments independent of activity. Instead, is controlled by the intracellular redox state, which itself depends on nature electron entry into Electron Transport Chain (ETC)—...

Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing Δubp3 cells, orthogonal approaches,...

Abstract Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing Δubp3 cells, orthogonal...

Abstract Cells require disparate amounts of distinct amino acids, which themselves have discrete biosynthetic costs. However, it remains unclear if and how cells respond differently to their scarcity. To explore this, we re-organized acids into groups based on metabolic origins. Subsequently, using yeast assessed responses transient disruptions in acid supply, uncover diverse restoration for acids. hierarchically prioritize restoring glutamate-, sulfur-, pentose-phosphate- pyruvate-derived...

Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing Δubp3 cells, orthogonal approaches,...