A5067286292- Algal biology and biofuel production
- Micro and Nano Robotics
- Chemical and Physical Properties in Aqueous Solutions
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- Crystallization and Solubility Studies
- Advanced Electron Microscopy Techniques and Applications
- Medical Imaging Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Microplastics and Plastic Pollution
- 3D Printing in Biomedical Research
- Seaweed-derived Bioactive Compounds
- RNA Interference and Gene Delivery
- Electrohydrodynamics and Fluid Dynamics
- Microbial Community Ecology and Physiology
- Effects and risks of endocrine disrupting chemicals
- Winter Sports Injuries and Performance
- Protein purification and stability
- Adventure Sports and Sensation Seeking
- Outdoor and Experiential Education
- Computational Geometry and Mesh Generation
- Advanced Fluorescence Microscopy Techniques
- Advanced Graph Theory Research
- Analytical Chemistry and Chromatography
- Molecular Communication and Nanonetworks
University of California, San Diego
2016-2025
Ronin Institute
2018-2025
Universidade de São Paulo
2013-2022
University of Zurich
2021
University of San Diego
2017
Universidade Federal de São Paulo
2016
Universidade Cidade de São Paulo
2013
Associação de Pais e Amigos dos Excepcionais de São Paulo
2012
Our reliance on agriculture for sustenance, healthcare, and resources has been essential since the dawn of civilization. However, traditional agricultural practices are no longer adequate to meet demands a burgeoning population amidst climate-driven challenges. Microalgae emerge as beacon hope, offering sustainable renewable source food, animal feed, energy. Their rapid growth rates, adaptability non-arable land non-potable water, diverse bioproduct range, encompassing biofuels...
ABSTRACT Chlamydomonas reinhardtii is a promising model microalga for recombinant molecules production. Nonetheless, low yield challenge its industrial use. This work investigated the influence of ammonium chloride (NH 4 Cl) concentration and temperature on growth transgenic C. expressing fluorescent protein mCherry laboratory scale. A Central Composite Rotatable Design was used to establish cultivation conditions. NH Cl concentrations ranging from 400 647.49 mg/L temperatures between 25°C...
Efficient protein secretion is a desirable trait for any recombinant expression system, together with simple, low-cost, and defined media, such as the typical media used photosynthetic cultures of microalgae. However, low titers secreted heterologous proteins are usually obtained, even most extensively studied microalga Chlamydomonas reinhardtii, preventing their industrial application. In this study, we aimed to expand evaluate secretory signal peptides (SP) in C. reinhardtii by comparing...
Abstract Algae biotechnology holds immense promise for revolutionizing the bioeconomy through sustainable and scalable production of various bioproducts. However, its development has been hindered by lack advanced genetic tools. This study introduces a synthetic biology approach to develop such tools, focusing on construction testing promoters. By analyzing conserved DNA motifs within promoter regions highly expressed genes across six different algal species, we identified cis-regulatory...
We present the comprehensive characterization of a newly identified microalga, Chlamydomonas pacifica, originally isolated from soil sample in San Diego, CA, USA. This species showcases remarkable biological versatility, including broad pH range tolerance (6-11.5), high thermal (up to 42 degree celsius), and salinity resilience 2% NaCl). Its amenability genetic manipulation sexual reproduction via mating, particularly between two opposing strains CC-5697 & CC-5699, now publicly available...
Microalgae offer a compelling platform for the production of commodity products, due to their superior photosynthetic efficiency, adaptability nonarable lands and nonpotable water, capacity produce versatile array bioproducts, including biofuels biomaterials. However, scalability microalgae as bioresource has been hindered by challenges such costly biomass related vulnerability pond crashes during large-scale cultivation. This study presents pipeline genetic engineering pilot-scale biodiesel...
Harnessing and controlling the localization of recombinant proteins is critical for advancing applications in synthetic biology, industrial biotechnology, drug delivery. This study explores protein anchoring controlled release Chlamydomonas reinhardtii , providing innovative tools these fields. Using truncated variants GP1 glycoprotein fused to plastic-degrading enzyme PHL7, we identified PPSPX motif as essential cell wall. Constructs with increased content exhibited reduced secretion but...
Chlamydomonas incerta , a genetically close relative of the model green alga reinhardtii shows significant potential as host for recombinant protein expression. Because genetic relationship between C. and this species offers an additional reference point advancing our understanding photosynthetic organisms, also provides new candidate biotechnological applications. This study investigates ’s capacity to express three proteins: fluorescent mCherry, hemicellulose-degrading enzyme xylanase,...
Sustainable low-carbon energy solutions are critical to mitigating global carbon emissions. Algae-based platforms offer potential by converting dioxide into valuable products while aiding sequestration. However, scaling algae cultivation faces challenges like contamination in outdoor systems. Previously, our lab evolved Chlamydomonas pacifica, an extremophile green alga, which tolerates high temperature, pH, salinity, and light, making it ideal for large-scale bioproduct production,...
Bioprospecting algae strains with tolerance to extreme conditions such as pH, temperature, salinity, and light is crucial for advancing biotechnology environmental applications. However, traditional screening methods often involve significant costs labor, restricting their accessibility practical use. In this study, we developed validated low-cost, high-throughput techniques, predominantly employing agar plates liquid culture assays, effectively differentiate levels among various strains....
The discovery of a new extremophile alga, Chlamydomonas pacifica, provides an opportunity to expand on heterologous protein expression beyond the traditional reinhardtii. C. pacifica is unicellular capable surviving at high pH, temperatures, and salinity. These various growth conditions allow outcompete any invading contaminants in open-air environments. Developing this novel species as platform for recombinant production could significantly advance commercial microalgal production. We have...
This review aimed to present Chlamydomonas reinhardtii as an alternative for heterologous protein production, especially biopharmaceuticals, and its general characteristics when compared with other expression systems. The need produce proteins industrial interest, therapeutic ends, diagnostic kits has led the development of recombinant microalgal technology. technology presents some interesting features, such rapid growth low transgene dispersion plants, ability fold complex bacteria,...
Algae biotechnology holds immense promise for revolutionizing the bioeconomy through sustainable and scalable production of various bioproducts. However, their development has been hindered by lack advanced genetic tools. This study introduces a synthetic biology approach to develop such tools, focusing on construction testing promoters. By analyzing conserved DNA motifs within promoter regions highly expressed genes across six different algal species, we identified cis-regulatory elements...
Abstract Microalgae offer a compelling platform for the production of commodity products, due to their superior photosynthetic efficiency, adaptability non-arable lands and non-potable water, capacity produce versatile array bioproducts, including biofuels biomaterials. However, scalability microalgae as bioresource has been hindered by challenges such costly biomass related vulnerability pond crashes during large-scale cultivation. This study presents pipeline genetic engineering...
Abstract Plastic pollution has become a global crisis, with microplastics contaminating every environment on the planet, including our food, water, and even bodies. In response, there is growing interest in developing plastics that biodegrade naturally, thus avoiding creation of persistent microplastics. As mechanism to increase rate polyester plastic degradation, we examined potential using green microalga Chlamydomonas reinhardtii for expression secretion PHL7, an enzyme breaks down...