A5079624513- Platelet Disorders and Treatments
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Biochemical Acid Research Studies
- Cell Adhesion Molecules Research
- Diet, Metabolism, and Disease
- Renal Diseases and Glomerulopathies
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Ion Transport and Channel Regulation
- Blood disorders and treatments
- Alzheimer's disease research and treatments
- Ion channel regulation and function
- Lipid metabolism and biosynthesis
- Amino Acid Enzymes and Metabolism
- Blood groups and transfusion
- RNA and protein synthesis mechanisms
- Blood properties and coagulation
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Hemoglobin structure and function
Polytechnic Institute of Bragança
2023
Centro de Investigaciones Biológicas Margarita Salas
2002-2018
Centre for Biomedical Network Research on Rare Diseases
2009-2018
Instituto de Investigación de Enfermedades Raras
2008-2018
Consejo Superior de Investigaciones Científicas
1995-2013
Centro de Investigación Biomédica en Red
2008-2009
Hospital General Universitario Gregorio Marañón
1974-1986
National University of Distance Education
1986
Universidad de Extremadura
1981
Unidades Centrales Científico-Técnicas
1976
The effect of glucagon on hepatic protein systhesis and proteolysis has been investigated. intraperitoneal administration 200 mug produced an increase the polypeptide chains completion time which was maximal 5 min after its approached control values at 20 min. polypeptides observed hormone represents a 38% inhibition synthetic rate. When continuously supplied by intravascular infusion, attained throughout experiment. This synthesis brought about accompanied in polyribosomal state...
The metabolic effects of glucagon and plus insulin on the isolated rat livers perfused with 10 mM sodium L-lactate as substrate were studied. Glucagon stimulated gluconeogenesis, ketogenesis ureogenesis at concentration used 2.1 nM. addition to give a glucagon-to-insulin ratio 0.2 reversed all effects. enhancement gluconeogenesis was accompanied by rise in cytosolic mitochondrial state reduction NAD system fall [ATP]/[ADP] ratio. analysis intermediary metabolite concentrations suggested,...
The binding of phenylalanyl‐tRNA to purified yeast ribosomes at low magnesium concentrations is dependent upon one the supernatant transfer factors (factor A). This reaction requires GTP. GTP analogue guanylyl methylene diphosphonate cannot be substituted for but it acts as a good inhibitor. After incubation with phenylalanyl‐tRNA, factor A and practically no compound other than itself found linked ribosomes. In addition this enzymic type binding, also bound in absence provided that...
Abstract Background Podocalyxin (podxl) is a heavily glycosylated transmembrane protein mainly found on the apical membrane of rat podocytes and also in endothelial, hematopoietic, tumor cells. Despite its interest no much known about transcriptional regulation podxl different Thus, we aimed at studying functional features 5'-regulatory region human Podxl gene. Results The promoter gene has been cloned structure function were analyzed. primary DNA sequence rich G+C devoid TATA or CAAT boxes....
We investigated the effects of administration single amino acids to starved rats on regulation protein synthesis in liver. Of all tested, only alanine, ornithine and proline promoted statistically significant increases extent hepatic polyribosome aggregation. The most effective these was whose effect promoting polyribosomal aggregation accompanied by a decrease polypeptide-chain elongation time. following observations indicate that alanine plays an important physiological role synthesis....
Abstract Epstein‐Barr–transformed lymphocytes from Alzheimer's disease patients showed the following distinct features in controlling intracellular pH compared with cells normal age‐matched controls: (1)The αIgM‐induced acidification was more pronounced than control and this effect appears to be associated a loss of effectiveness Ca 2+ / calmodulin‐dependent mechanism activity Na + /H exchanger; (2) H ‐buffering capacity rate proton efflux response an acid load were both decreased cells. It...
This work reports the structural and functional characterization of platelet glycoprotein complex GPIIb-IIIa (integrin alpha IIb beta 3) in a patient type II Glanzmann thrombasthenia, bearing homozygous G-->A base transition at position 1074 GPIIb that results an Arg327-->His substitution. CHO cells stably transfected with cDNA encoding His327GPIIb showed drastic reduction surface expression 3 relative to control wild GPIIb. Immunoprecipitation analysis demonstrated synthesis,...
ImageB]>>/Type/Page>> endobj 42 0 obj <> 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 <>stream HWrH}WÌ£Ù²aeª™G" Y[PK}m%eKA`ohF#)JH>=6 "?J|ÚŸ&SÞ"'unDQTHÔ˜0P(>&4DÚži{a=GFo~qs`!4 x `Q@̸XE\wÒ ‡5 6I_&* =rogC<=(5H*yZSmG^&)}Z8G&S0Y4~>n)Ö'è‚-wgSMG\å±¼||x^>d\g5aq*z2>`Ü®WUi.51Co~]/g'DO1jAÉž-αcÜOEObË ‡r0i QAEŸLr3usgNqKh FK#{F>h1DuqSE2u08n`
The effect of nucleotide energy levels in vivo on the different steps protein synthesis has been studied. Hepatic anoxia was induced by interrupting blood portal-vein flow. At 5 min ATP fell to 59% control values and amino acid incorporation into inhibited more than 70%. This strong inhibition not paralleled polyribosomal breakdown. On contrary, when fasted rats were used, at ribosomal state aggregation found increase. Longer periods resulted a further decrease triphosphonucleoside content...