Journal Article Intercontinental Spread of a Multiresistant Clone Serotype 23F Streptococcus pneumoniae Get access Rosario Munoz, Munoz Search for other works by this author on: Oxford Academic PubMed Google Scholar Tracey J. Coffey, Coffey Margaret Daniels, Daniels Christopher G. Dowson, Dowson Gotz Laible, Laible Julio Casal, Casal Regine Hakenbeck, Hakenbeck Michael Jacobs, Jacobs James M. Musser, Musser Brian Spratt, Spratt ... Show more Alexander Tomasz The Infectious Diseases, Volume...

Summary Multiply antibiotic‐resistant serotype 23F isolates of Streptococcus pneumoniae are prevalent in Spain and have also been recovered recently the United Kingdom States. Analysis populations these by multilocus enzyme electrophoresis, restriction endonuclease cleavage electrophoretic profiling penicillin‐binding protein (PBP) genes, has demonstrated that a single clone (Muñoz et al. , 1991). Here we report studies non‐serotype penicillin‐resistant pneumococci isolated Kingdom. One...

Serotype 19F variants of the major Spanish multiresistant serotype 23F clone Streptococcus pneumoniae have been proposed to arisen by recombinational exchanges at capsular biosynthetic locus. Members and were confirmed be essentially identical in overall genotype, as they indistinguishable REP‐PCR, had sequences three polymorphic housekeeping genes. Eight studied all large replacements In cases, one cross‐over points appeared upstream dexB which flanks end locus, six other point was...

Summary Penicillin‐resistant strains of Streptococcus pneumoniae possess forms penicillin‐binding proteins (PBPs) that have a low affinity for penicillin compared to those from penicillin‐sensitive strains. PBP genes penicillin‐resistant isolates are very variable and mosaic structure composed blocks nucleotides similar found in differ by up 21%. These chromosomally encoded presumably arisen following transformation homologous recombination with number closely related species. This study...

Summary Resistance to third‐generation cephalosporins in a clinical isolate of Streptococcus pneumoniae was shown be due the production altered forms penicillin‐binding proteins (PBPs) 2X and 1A. The cloned PBP2X gene from resistant strain able transform susceptible an intermediate level resistance. resulting transformant could transformed full resistance using PBP1A latter strain. Chromosomal DNA (and other strains) readily (>250‐fold for cefotaxime; >100‐fold ceftriaxone) single step...

Streptococcus pneumoniae CS109 and CS111 were isolated in the United States 1991 have high levels of resistance to expanded-spectrum cephalosporins (MICs 8 32 micrograms cefotaxime per ml, respectively). CS109, but not CS111, also showed high-level penicillin. As both strains expressed serotype 23F capsule, very closely related overall genotype, possessed identical or mosaic pbp1a, pbp2x, pbp2b genes, it is likely that they arisen from a recent common ancestor. High-level was entirely due...

ABSTRACT The genetic relatedness and evolutionary relationships between group B streptococcus (GBS) isolates from humans those bovines were investigated by phylogenetic analysis of multilocus sequence typing data. collection consisted 111 GBS cows with mastitis a diverse global patients invasive disease ( n = 83) carriers 69). Cluster showed that the majority bovine (93%) grouped into one cluster. human greater diversity clustered separately population. However, homogeneous type 17 (ST-17)...

Mosaic penicillin-binding proteins (PBP) 1A, 2X and 2B genes were cloned from four clinical isolates of Streptococcus pneumoniae with levels susceptibility to penicillin ranging 1.5 16 micrograms benzylpenicillin ml-1. In each instance it was possible transform either the penicillin-sensitive laboratory strain R6 or a sensitive isolate 110K/70 full level resistance these three alone. Until now has not been clearly determine whether alterations PBP1A, alone sufficient attain high resistance.

The high prevalence of penicillin resistance among Streptococcus pneumoniae isolates from Uruguay has been associated with the emergence a penicillin-resistant clone serotype 14. Isolates this were identical by multilocus sequence typing to members Spanish 9V and possessed indistinguishable forms penicillin-binding protein 2b 2x genes. Their pbp1a genes also identical, except at 3′ end. 14 shown be variant which arose 22·2 kb recombinational replacement that introduced capsular biosynthetic...

Streptococcus pneumoniae (the pneumococcus) is believed to have developed resistance penicillin by the production of altered forms penicillin-binding proteins (PBPs) that decreased affinity for penicillin. Sixty-eight clinical isolates serogroup 6 and 19 pneumococci (MICs, < 0.015 8 micrograms/ml) were randomly selected from hospitals across South Africa which are at substantial geographic distance each other. The polymerase chain reaction was used isolate domain PBPs 2B 2X chromosomal...

ABSTRACT Intramammary infection with Streptococcus uberis is a common cause of bovine mastitis throughout the world. Several procedures to differentiate S. isolates have been proposed. However, all are prone interlaboratory variation, and none suitable for description population structure. We describe here development multilocus sequence typing (MLST) scheme help address these issues. The sequences seven housekeeping gene fragments from each 160 United Kingdom milk were determined. Between 5...

The region encoding the transpeptidase domain of penicillin-binding protein 2B (PBP 2B) gene two penicillin-resistant clinical isolates Streptococcus oralis was > 99.6% identical in nucleotide sequence to that a serotype 6 isolate pneumoniae. downstream 849 base pairs these genes were identical. Analysis data indicates PBP has probably been transferred from S. pneumoniae into oralis, rather than vice versa, and shows one this resistance distributed horizontally both within different viridans...

A total of 26% the pneumococci isolated from an outpatient clinic in Nairobi, Kenya, during 1991 to 1992 had intermediate levels penicillin resistance. Gene fingerprinting and DNA sequencing were used distinguish penicillin-binding protein (PBP) 1A, 2B, 2X genes 23 resistant isolates. Isolates grouped into those that identical forms each three PBP (fingerprint groups) rRNA gene restriction patterns (ribotypes). Both methods divided isolates 11 groups. In a few cases, horizontal transfer...

We analysed a collection of 95 multiply antibiotic-resistant pneumococci, recovered since 1988 from 14 Spanish hospitals, that have MICs ≥ 0·25 µg benzylpenicillin ml−1. The majority the isolates were serogroups 14, 23, 6, 19 and 15, which are currently mainly associated with multiresistance in Spain. All serogroup 23 members major serotype 23F multiresistant clone. Similarly, most 6 6B clone, or variants this Eighteen 24 highly penicillin-resistant clone appears to be 19F variant 25...

Streptococcus uberis, strain 0140J, contains a single copy sortase A (srtA), encoding transamidase capable of covalently anchoring specific proteins to peptidoglycan. Unlike the wild-type, an isogenic mutant carrying inactivating ISS1 insertion within srtA was only able infect bovine mammary gland in transient fashion. For first 24 h post challenge, colonised at similar rate and number wild type strain, but unlike did not subsequently colonise higher numbers. Similar levels host cell...

ABSTRACT TLRs mediate recognition of a wide range microbial products, including LPS, lipoproteins, flagellin, and bacterial DNA, signaling through leads to the production inflammatory mediators. In addition TLRs, many other surface receptors have been proposed participate in innate immunity recognition, some these, for example, C-type lectins, is likely cooperate with TLR defining responses. present study, we examined importance ECD intracellular TIR domain boTLR2 huTLR2 induce...

Abstract IL-17 has emerged as a key player in the immune system, exhibiting roles protection from infectious diseases and promoting inflammation autoimmunity. Initially thought to be CD4 T-cell-derived, sources of are now known varied belong both innate adaptive arms system. Mechanisms for inducing production lymphoid cells rely on appropriate antigenic stimulation context TGF-β1, IL-6 and/or IL-1β. Using culture protocols adapted human studies, we have effectively induced bovine + WC1 γδ...

Streptococcus uberis, a Gram-positive, catalase-negative member of the family Streptococcaceae is an important environmental pathogen responsible for significant proportion subclinical and clinical bovine intramammary infections. Currently, genome only single reference strain (0140J) has been described. Here we present comparative analysis complete draft sequences additional twelve S. uberis strains.Pan core revealed common to all strains be 1,550 genes in 1,509 orthologous clusters,...