A5030579536- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- biodegradable polymer synthesis and properties
- Microbial metabolism and enzyme function
- Anaerobic Digestion and Biogas Production
- Plant biochemistry and biosynthesis
- Microbial Metabolism and Applications
Engineering and Physical Sciences Research Council
2021-2024
Biotechnology and Biological Sciences Research Council
2021-2024
University of Nottingham
2020-2024
Stable production of value-added products using a microbial chassis is pivotal for determining the industrial suitability engineered biocatalyst. Microbial cells often lose multicopy expression plasmids during long-term cultivations. Owing to advantages related titers, yields, and productivities when system compared with genomic integrations, plasmid stability essential industrially relevant biobased processes. Cupriavidus necator H16, facultative chemolithoautotrophic bacterium, has been...
3-Hydroxypropionate (3-HP) is a versatile compound for chemical synthesis and potential building block biodegradable polymers. Cupriavidus necator H16, facultative chemolithoautotroph, an attractive production chassis has been extensively studied as model organism biopolymer production. Here, we engineered C. H16 3-HP biosynthesis from its central metabolism. Wild type can use carbon source, highly undesirable trait chassis. However, deletion of three (methyl-)malonate semialdehyde...
Polyhydroxyalkanoates are attractive alternatives to traditional plastics. However, although polyhydroxybutyrate (PHB) is produced in large quantities by Cupriavidus necator H16, its properties far from ideal for the manufacture of plastic products. These may be improved through coproduction with 3-hydroxypropionate (3HP), which leads formation copolymer poly(3-hydroxybutyrate-co-3-hydroxypropionate) (poly(3HB-co-3HP). To achieve this, a pathway was designed enable C. H16 convert β-alanine...