A5059956562- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Identification and Quantification in Food
- Forensic Anthropology and Bioarchaeology Studies
- Environmental DNA in Biodiversity Studies
- Genetic diversity and population structure
- Yersinia bacterium, plague, ectoparasites research
- Genetic Associations and Epidemiology
- Wildlife Ecology and Conservation
- Historical and Archaeological Studies
- Biosensors and Analytical Detection
- Race, Genetics, and Society
- Insect and Pesticide Research
- Genomics and Phylogenetic Studies
- Advanced Chemical Sensor Technologies
- Nutrition, Genetics, and Disease
- Paleopathology and ancient diseases
- Ancient and Medieval Archaeology Studies
- Archaeology and ancient environmental studies
- Eosinophilic Disorders and Syndromes
- Isotope Analysis in Ecology
- Digital and Cyber Forensics
- Chemical Safety and Risk Management
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
Fakultní nemocnice Bulovka
2016-2025
Forensic DNA Service (Czechia)
2014-2025
Charles University
2011-2025
Police of the Czech Republic
1996-2003
University Psychiatric Hospital
2000
AimTo develop novel DNA extraction and typing procedure for identification of the 7th century human remains, determine familiar relationship between individuals, estimate Y-chromosome haplogroup, compare haplotype with contemporary populations.MethodsDNA from preserved femur samples was extracted using modified silica-based technique. Polymerase chain reaction amplification performed kits MiniFiler, Identifiler, Y-filer also laboratory-developed validated short tandem repeat (STR)...
Illegal wildlife trade is currently on the rise, and it becoming one of most lucrative crime sectors. The rarer species, higher demand. Wildlife falls under international regulations, such as CITES convention. Proving that this convention has been violated a complex process can be very difficult to do. DNA analysis methods remain (in many cases) only way determine whether certain specimen originated from protected animal specific individual, or species in which legal trade. Tanned hides are...
This study describes the development of a species determination/DNA quantification system called Pleo Qplex and an individual identification STR multiplex STRplex using Panthera leo samples. enables us to measure quantity extracted nuclear mitochondrial DNA detect presence co-purified inhibitors. STRplex, consisting seven loci, determination profile from sample based on analysis short tandem repeats (STRs). The provides additional loci top previously published in Ptig contains specific...
The Eurasian lynx (Lynx lynx) is listed in CITES Appendix II and protected under the Bern Convention EU Habitats Directive, yet it remains a frequent target of wildlife crime, highlighting urgent need for reliable identification methods. This study focuses on determination DNA quantification Lynx spp. using quantitative real-time PCR (qPCR). Llynx Qplex multiplex system effectively distinguishes from other Feliformia species by targeting mitochondrial nuclear markers. Additionally, we...
This study aimed to provide an overview of the methodological approach used for species determination big cats. The molecular system described herein employs mitochondrial DNA control region (CR-mtDNA)-length polymorphism in combination with highly sensitive and precise capillary electrophoresis. We demonstrated that CR-mtDNA barcoding can be utilized where presence biological material from cats is expected or as a confirmatory test alongside Sanger massive parallel sequencing (MPS). have...
AimA collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and Institute of Legal Medicine, 2nd Faculty Charles University in Prague, Czech Republic, aim to test performance different laboratories carrying out analysis relatively old bone samples.MethodsEighteen participating were asked perform typing two samples powder. Two provided National Museum Archaelogy came from archeological excavations estimated be approximately 150 400 years old. The...
AimTo evaluate the novel triplex polymerase chain reaction (PCR) assay for analysis of polymorphic Y-chromosomal short tandem repeat loci (Y-STR).MethodsA total 14 Y-STR was analyzed. Allele frequencies 3 tetrameric (DYS449, DYS456, and DYS458) extended haplotype typed by Y-PLEXTM 12 system were investigated in a sample 50 unrelated healthy Czech male donors. We computed relevant intra-population statistic parameters our data (gene diversity, average gene diversity over loci, mean number...
AimTo evaluate Y-chromosomal diversity of the Moravian Valachs Czech Republic and compare them with a population sample other samples from Central South-Eastern Europe, to effects genetic isolation sampling.MethodsThe first set consisted 94 unrelated male donors Valach region in northeastern border-area. The second 79 men who originated 7 paternal lineages defined by surname. No close relatives were sampled. third 273 whole was used for comparison, as well published data 27 populations....
Skeletal remains are among the most difficult types of samples encountered in forensic DNA casework and historical investigations due to prolonged exposure environmental insults. extracted from bone often is degraded, low quantities, contains co-purified inhibitors surrounding soil and/or burial vault material. When sexually dimorphic skeletal elements not recovered, determining sex a decedent can be challenging. With unidentified human remains, genetic data evaluated concert with...
Aim: To evaluate the performance of a police laboratory that specializes in DNA identification skeletal remains terms success rates and compliance procedures with international quality assurance standards recommendations; to describe correlations between sample type, post-mortem interval level decomposition ostheological samples autosomal STR typing dataset 219 individuals (323 samples) Slovakia; provide readers comprehensive guidelines for set-up operation specialized bone laboratory.This...
The aim of this technical note is to provide an overview methodical approaches used develop molecular systems for species determination/DNA quantification called Ptig Qplex and individual identification STRplex Panthera tigris samples. Both will help combat the illegal trade endangered create a worldwide shared database DNA profiles.