Background Obstructive sleep apnea (OSA) is a common disorder, frequently observed in obese individuals, with shared mechanisms involving leptin and its receptor, which regulate appetite energy expenditure. SH2B1 key enhancer of signaling the receptor pathway. This study aimed to investigate association between variants OSA.

Abstract Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide. Natural compounds with anticancer potential, such as tomentosin, sesquiterpene lactone derived from Inula viscosa , are under investigation alternative therapeutic agents. However, its potential effects on CRC remain unexplored. This study aimed to evaluate the tomentosin in cells and elucidate underlying molecular mechanisms. HCT 116 HT- 29 were treated cell viability, colony formation, invasion,...

Abstract The activity concentrations of natural radionuclides in peloids were studied to assess the radiologic hazard from 18 Turkish spas. are mainly used for therapeutic treatments, rheumatic diseases and aesthetic purposes. 226 Ra, 232 Th, 40 K 137 Cs determined with a gamma ray spectrometer using HPGe detector. average K, 110.69, 71.52, 576.48 0.447 Bq/kg, respectively. radium equivalent activities peloid samples ranged 63.3 766.77 Bq/kg. absorbed dose rate (D out ) varied between 37.52...

Abstract The aim of this study was to evaluate the anticancer effects piceatannol, a natural stilbenoid, on human neuroblastoma cells. In order accomplish goal, we performed various cellular assays, including XTT cell proliferation assay for viability, colony formation capacity, FITC Annexin V and death detection kit apoptosis, matrigel invasion intracellular reactive oxygen species (ROS) red dye ROS levels, TMRM staining method mitochondrial membrane potential (MMP), CYTO‐ID autophagy...

Lactic acid bacteria are very significant to human health  due the production of some antimicrobial substances and ability inhibit pathogenic bacteria. Furthermore, also used as starter culture in various food production. In this study,Lactobacillus strains were isolated from 100 milk colostrum samples 50 faecal 3 30-day old infants who fed on only breast milk. The isolatedLactobacillus strains futher identified by API CHL systems as Lactobacillus acidophilus (20.0%), L. acidophilus -3...

RT-qPCR was used to analyze the vitamin D receptor (VDR) gene TaqI polymorphism in 100 Turkish patients with type 2 diabetes mellitus (T2DM) and hypertension compared healthy subjects, determine whether VDR could be considered as one of susceptibility genes for T2DM hypertension.Genotyping done PCR, followed by melting curve analysis specific fluorescent hybridization probes.The results showed that distributions TT, Tt tt genotypes were 51, 46 3% patient group, 35, 49 16% control...

Herbal and aromatic plants are attracting more attention among contemporary plant researchers because some human diseases resulting from bacterial antibiotic resistances have gained worldwide concern. A number of methods available being developed for the isolation nucleic acids plants. Because contain high amounts many different substances, it is unlikely that just one acid method suitable all can ever exist. Therefore, we 4 modified new produced good quality DNA these This article deals...

Background/aim: The purpose of the present study was to investigate whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in prostate cancer (PCa). Materials and methods: We examined three eNOS (T-786C promoter region, G894T, Intron 4 VNTR 4a/b) at extracted DNAs from 50 formalin-fixed paraffin-embedded tissues PCa patients. For controls, blood samples obtained healthy men were studied. Genotyping molecular variants performed by PCR-RFLP technique. Results: found...

The thermal properties such as specific heat capacity and dissipation of peloids play an important role in therapeutic applications. Therefore, these were determined on the samples taken from spas where peloid applications are used Turkey. In addition, some pure clay minerals investigated to determine their suitability possible constituents make recommendations for preparing most usable therapy. For this aim, mineralogical using X-ray diffraction (XRD) Differential Scanning Calorimeter...

We aimed molecular analysis of soybean by lecithin specific primer pairs LE5/LE6. Genomic DNA was extracted from CTAB method and EZ1 nucleic acid isolation system. A sensitive qualitative detection for soybean, using the polymerase chain reaction developed with E5/LE6 primers, produced a 195 bp product. However, antioxidant activity methanolic extract Turkey DPPH ABTS radical scavenging assays, showed higher 53.19±0.87% 45.10±0.32%, respectively. Fatty compositions several phenolic acids...

One prerequisite to reliable molecular biology work is that the genomic DNA of a sample should be good quality. The isolation intact, high-molecular-mass essential for many applications including long Polymerase Chain Reaction (PCR), endonuclease restriction digestion, Southern blot analysis, and library construction. Many protocols are available extraction from plant material. of Ginkgo biloba is quite difficult on because high phenolic polysaccharide content its leaves. This study aimed...

Abstract Globally colorectal cancer ranks as the third most widespread disease and leading cause of cancer-associated mortality. Immunotherapy treatments like PD-L1 blockade have been used to inhibit legend, which boosts activity cytotoxic T lymphocytes. Recently, studies suggest that some probiotics could potentially enhance effectiveness immunotherapy for patients. We found in Caco-2 HT-29 cells, live Leuconostoc mesenteroides treatment resulted an increase expression this stimulated...

Genetic analysis of plants relies on high yields pure DNA samples. Here we present the optimization isolation and PCR conditions for RAPD selected medicinal conservation concern from Turkey, containing levels polysaccharides, polyphenols secondary metabolites. The method involves a modified CTAB extraction employing polyvinyl pyrrolidone (PVP) while grinding, successive long-term chloroform-isoamylalcohol extractions, EZ1 nucleic acid protocols. yield ranged 1 - 2 μg/μl per gram leaf tissue...

The efficiency, with which the lysis of 20 anaerobic bacterial samples [8 different species isolated from 4 colostrum and infants (which are fed only by breast milk 3 to 30-day-old) faeces] were lysed, was tested using a reference or an improving method that involves higher levels lysozyme (Sigma-Aldrich) proteinase K solution (Sigma-Aldrich). Eight lysed efficiency > 87% methods. Also, lytic for Bifidobacterium bifidum BB-12 90%. Accordingly, since DNA improved methods can reflect nearly...

ABSTRACTChickpea is an important food legume crop with high nutritional value. Lack of suitable DNA isolation protocol a limiting factor for any molecular studies on this crop. Today, however, it possible to determine plants that possess desired qualities in earlier generations using methods (DNA marker techniques) and combine gene areas bearing the commercially used varieties. Therefore, later studies, 5 ISSR primers, which were pre-determined chickpea genome mapping characteristic...

Diabetes Mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia resulting from defects insulin secretion and/or increased cellular resistance to insulin. Chronic and other disturbances DM lead long-term tissue organ damage as well dysfunction involving the eyes, kidneys, nervous vascular systems. Type 2 mellitus; most common form worldwide, its prevalence increasing. Its underlying can vary predominant with relative deficiency secretory defect resistance. Transcription...