A5074788664- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Peroxisome Proliferator-Activated Receptors
- Redox biology and oxidative stress
- Heat shock proteins research
- Adenosine and Purinergic Signaling
- Signaling Pathways in Disease
- Fermentation and Sensory Analysis
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
Jagiellonian University
2018-2024
Postgraduate School of Molecular Medicine
2018-2024
University of Nottingham
2015-2019
Article9 September 2020Open Access Transparent process Molecular basis for the bifunctional Uba4–Urm1 sulfur-relay system in tRNA thiolation and ubiquitin-like conjugation Marta Pabis orcid.org/0000-0001-7394-5206 Malopolska Centre of Biotechnology (MCB), Jagiellonian University, Krakow, Poland Search more papers by this author Martin Termathe orcid.org/0000-0002-5618-4633 Max Planck Institute Biomedicine, Muenster, Germany Keerthiraju E Ravichandran orcid.org/0000-0003-2568-1343...
Abstract Post‐translational modifications by ubiquitin‐like proteins (UBLs) are essential for nearly all cellular processes. Ubiquitin‐related modifier 1 (Urm1) is a unique UBL, which plays key role in tRNA anticodon thiolation as sulfur carrier protein (SCP) and linked to the noncanonical E1 enzyme Uba4 (ubiquitin‐like activator 4). While Urm1 has also been observed conjugate target like other UBLs, molecular mechanism of its attachment remains unknown. Here, we reconstitute covalent...
Saccharomyces cerevisiae is the micro-organism of choice for conversion fermentable sugars released by pre-treatment lignocellulosic material into bioethanol. Pre-treatment releases acetic acid and previous work identified a cytochrome oxidase chaperone gene (COX20) which was significantly up-regulated in yeast cells presence acid.A Δcox20 strain sensitive to compared with background strain. Overexpressing COX20 using tetracycline-regulatable expression vector system strain, resulted...
The yeast peroxiredoxin Ahp1, like related anti-oxidant enzymes in other species, undergoes urmylation, a lysine-directed conjugation to ubiquitin-like modifier Urm1. Ahp1 assembles into homodimer that detoxifies peroxides via forming intersubunit disulfides between peroxidatic and resolving cysteines are subsequently reduced by the thioredoxin system. Although urmylation coincides with oxidative stress, it is unclear how this modification happens on molecular level whether affects activity....
Industrial production of bioethanol from lignocellulosic materials (LCM's) is reliant on a microorganism being tolerant to the stresses inherent fermentation. Previous work has highlighted importance cytochrome oxidase chaperone gene (COX20) in improving yeast tolerance acetic acid, common inhibitory compound produced during pre-treatment LCM's. The presence acid been shown induce oxidative stress and programmed cell death, so role COX20 was determined. Analysis using flow cytometry revealed...
Ubiquitin-related modifier 1 (Urm1) is a highly conserved member of the ubiquitin-like (UBL) family proteins. Urm1 key component eukaryotic transfer RNA (tRNA) thiolation cascade, responsible for introducing sulfur at wobble uridine (U34) in several tRNAs. must be thiocarboxylated (Urm1-SH) by its E1 activating enzyme UBL protein activator 4 (Uba4). Uba4 first adenylates and then thiocarboxylates C-terminus using adenyl-transferase (AD) rhodanese (RHD) domains. However, detailed mechanisms...