A5091815534- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Skin and Cellular Biology Research
- Hair Growth and Disorders
- Skin Protection and Aging
- Corneal surgery and disorders
- melanin and skin pigmentation
- Mast cells and histamine
- Wound Healing and Treatments
- Dermatology and Skin Diseases
- MicroRNA in disease regulation
- Protease and Inhibitor Mechanisms
- Dermatologic Treatments and Research
- Allergic Rhinitis and Sensitization
- RNA Interference and Gene Delivery
- Connexins and lens biology
- Asthma and respiratory diseases
- Hedgehog Signaling Pathway Studies
- Cell Adhesion Molecules Research
- Urticaria and Related Conditions
- Advancements in Transdermal Drug Delivery
- Axon Guidance and Neuronal Signaling
- Wnt/β-catenin signaling in development and cancer
- Contact Dermatitis and Allergies
- Ocular Disorders and Treatments
Northwestern University
2015-2025
National Institutes of Health
1993-2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
Center for Cancer Research
2018
KU Leuven
2018
The University of Texas Southwestern Medical Center
2018
Université Paris-Est Créteil
2018
National Cancer Institute
2018
ABSTRACT Using double labeling techniques, we studied the replication of corneal epithelial stem cells that reside exclusively in limbal zone, and their progeny transit amplifying cells. We show can be induced to enter DNA synthesis by wounding TPA. demonstrate existence a hierarchy TA cells; those peripheral cornea undergo at least two rounds before they become post-mitotic, whereas central are capable only one round division. However, cell cycle time these shortened number times replicate...
Two structurally distinct populations of basal keratinocytes, nonserrated and serrated, were observed in cynomolgus monkey human palm epidermis. Anatomical location, fine structural features, kinetic properties suggest that cells represent a stem cell population serrated help anchor the epidermis to dermis.
Skin development requires reactive oxygen species generated by mitochondria in keratinocytes.
The skin is repeatedly exposed to solar UV radiation. Long-term photodamage a consequence of cumulative radiation injury. Hence an examination the repetitive effects exposure more likely yield clues early alterations that lead photoaged than single exposure.We examined low-dose irradiation on human with aim identifying UVA-induced may have different wavelength dependence acute erythema.Areas lower part back were each suberythemal dose (0.5 minimal erythema [MED]) simulated (290 400 nm) and...
Despite their potential to regulate approximately one-third of the whole genome, relatively few microRNA (miRNA) targets have been experimentally validated, particularly in stratified squamous epithelia. Here we demonstrate not only that lipid phosphatase SHIP2 is a target miRNA-205 (miR-205) epithelial cells, but, more importantly, corneal epithelial-specific miR-184 can interfere with ability miR-205 suppress levels. This first example miRNA negatively regulating another maintain levels...
To understand better the role of mast cell secretory products in genesis inflammation, a system was developed for vitro degranulation human cells skin organ cultures. Within 2 hr after morphine sulfate-induced degranulation, endothelial lining microvessels adjacent to affected expressed an activation antigen important endothelial-leukocyte adhesion. Identical results were obtained when other secretagogues (anti-IgE, compound 48/80, and calcium ionophore A23187) used. Induction this abrogated...
When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for emissions, active efflux of by ABCG2/BCRP1 transporter causes certain cells appear as a segregated cohort, known side population (SP). Stem from several tissues have been shown possess SP phenotype. As lack specific surface markers has hindered isolation subsequent biochemical characterization epithelial stem this study sought determine existence expression ABCG2 in...
The anatomy of linear wrinkles ("crow's feet' and temporal frown lines), fine criss-cross the face wrinkling general body surface elderly persons, was studied by light scanning electron microscopy. No histological features distinguished various from surrounding skin. It concluded that wrinkle is a configuration change, like grooves worn into an old glove, without specific structural alterations at level. As regards pathogenesis, common setting found to be deterioration elastic tissue...
microRNA-205 (miR-205) and miR-184 coordinately regulate the lipid phosphatase SHIP2 for Akt survival signaling in keratinocytes. As PI3K-Akt pathway has also been implicated regulating actin cytoskeleton cell motility, we investigated role that these 2 miRNAs play keratinocyte migration. We used antagomirs (antago) to reduce levels of miR-205 primary human epidermal keratinocytes (HEKs) corneal epithelial (HCEKs) as well direct silencing using siRNA oligos. Treatment HEKs HCEKs with...
Epithelial cells changing from the granular stage of differentiation to horny are more numerous, and reveal sequential events transformation in finer detail rumen epithelium than other keratinizing epithelia thus far studied electron microscope. Studies such indicate that is initiated by release hydrolytic enzymes, as evidenced appearance lysosomes. As lysosomes increase number, nucleus, ribosomes, mitochondria, Golgi apparatus, mucous granules gradually degraded. Furthermore, marked changes...