We have identified a plasma membrane Na(+)/H(+) antiporter gene from tomato (Solanum lycopersicum), SlSOS1, and used heterologous expression in yeast to confirm that SlSOS1 was the functional homolog of AtSOS1. Using post-transcriptional silencing, we evaluated role played by long-distance Na(+) transport salt tolerance tomato. Tomato because its anatomical structure, more complex than Arabidopsis, agricultural significance. Transgenic plants with reduced exhibited growth rate compared...

The Ca(2+)-dependent SOS pathway has emerged as a key mechanism in the homeostasis of Na(+) and K(+) under saline conditions. We have identified functionally characterized gene encoding calcineurin-interacting protein kinase tomato, SlSOS2. On basis sequence similarity complementation studies yeast Arabidopsis, it can be concluded that SlSOS2 is functional tomato homolog Arabidopsis AtSOS2 operates signal transduction pathway. biotechnological potential to provide salt tolerance was...

The enzymes lipoxygenase and hydroperoxide lyase have been identified in strawberry (Fragaria × ananassa Duch.) var. Camarosa. Their subcellular localization, substrate preference, product specificity were determined mature fruits. activity of both was located mainly the microsomal fraction. Linolenic acid preferred for lipoxygenase, forming 13- 9-hydroperoxides this proportion 70:30. cleaves 13-hydroperoxide linoleic (13% relative activity) linolenic (100% acids to form hexanal...

Four strawberry (Fragaria ananassa × Duch.) varieties Oso Grande, Chandler, Tudla, and I-101) were studied in relation to their alcohol acyltransferase (AAT) activity. AAT activity profile during fruit development ripening was determined for each variety. The highest value corresponded which is also the variety showing earliest maximum peak of effect passive modified atmosphere (MA) storage on studied. An increase found strawberries stored under high CO2 (>30%). This higher level could be...

The fate of amino acids in relation to aroma biogenesis was studied strawberries using the vitro growth approach. This fruit presented differences level metabolization for different acids. Incubations with L-isoleucine gave rise an increase fourteen compounds this aroma, either not detected previously or constituents strawberry aroma. However, L-valine incubations did provide a significant change Strawberry feeding resulted 7-fold sum 2-methylbutanoate esters, and double production...

Furaneol, mesifurane, and furaneol glucoside contents during ripening of four strawberry varieties (Oso Grande, Chandler, Tudla, I-101) have been analyzed. Patterns furanone were similar for the varieties, amounts increasing to reach highest values at overripe stage. However, derivatives differed quantitatively among varieties. The amount shown by Oso Grande stage is so far reported (37.05 μg/g fw). content in mesifurane was found I-101 variety, stage, 23.5 13.2 fw, respectively. Results...

Several classes of seed proteins limit the utilisation plant in human and farm animal diets, while foods have much to offer sustainable intensification food/feed production health. Reduction or removal these could greatly enhance protein quality various strategies been used try achieve this with limited success. We investigated whether protease inhibitor mutations be exploited quality, availing induced mutant natural Pisum germplasm collections identify mutants, whilst acquiring an...

Strawberry fruits (Fragaria × ananassa Duch.), cultivar Camarosa, were harvested at green inmature stages and grown in vitro sucrose-containing solutions. The effect of exogenously added methyl jasmonate (JAMe, 50 μM) on development ripening strawberry was evaluated through different parameters. A significant increase respiratory activity ethylene production determined white pink strawberries treated with JAMe, while responses elicited from ripe overripe fruits. higher growth rate found...

Functional gluten-free biscuits enriched with commercial and landrace non-commercial chickpea flours were designed compared a traditional shortbread biscuit. They analyzed in sensory attributes, amino acid profile, antioxidant properties. Subsequently, the digested vitro to evaluate protein hydrolysis, bioaccessibility, phenolic compounds release, markers. The presence of provided golden color heightened biscuit hardness fracturability (especially non-commercial), increasing crispness...

Abstract BACKGROUND In the global socioeconomic context and current climate change scenario, investigating nutritional bioactive characteristics of landraces can provide interesting profiles with technological applications benefits for human health. The purpose this work was to improve our knowledge regarding phenolic composition several chickpea accessions preserved in a gene bank compared widely consumed cultivars, as first approach establish their potential interest. CIELab colour,...

The Ca2+-dependent SOS pathway has emerged as a key mechanism in the homeostasis of Na+ and K+ under saline conditions. We recently identified functionally characterized by complementation studies yeast Arabidopsis gene encoding calcineurin-interacting protein kinase tomato, SlSOS2.Citation1 also show evidences on biotechnological potential SlSOS2 conferring salt tolerance to transgenic tomato. increased salinity overexpressing plants is associated with higher sodium content stems leaves....

The presence of so-called anti-nutritional factors can reduce the bioavailability nutrients following consumption seeds which are otherwise an excellent source proteins, carbohydrates and micronutrients. Among proteins associated with negative effects on quality in pea (Pisum sativum L.) lectin, albumin 2 (PA2) trypsin inhibitors (TI). Here we have investigated impact these protein digestibility amino acid availability, using naturally occurring derived mutant lines lacking proteins....

The substrate specificity of alcohol acyltransferase (AAT) enzymes from different strawberry varieties was studied. Proteins with AAT activity fruits Fragaria x ananassa Duch. cv. Oso Grande were purified to apparent homogeneity and used for kinetic studies straight-chain alcohols acyl-CoAs. K(m) values obtained enzyme six alcohols, using acetyl-CoA as cosubstrate, decreased increasing length the chain. In similar experiments increase in acyl-CoA carbon chain also found be correlated a...

Legume consumption has been reported to induce beneficial effects on obesity-associated metabolic disorders, but the underlying mechanisms have not fully clarified. In current work, pea (Pisum sativum L.) seed meal proteins (albumins, legumins and vicilins) were isolated, submitted a simulated gastrointestinal digestion, of their hydrolysates (pea albumins (PAH), (PLH) vicilin (PVH), respectively) 3T3-L1 murine pre-adipocytes investigated. The hydrolysate native vicilins, increased lipid...

Whey-based beverages could be an effective way of reusing a by-product th cheese industry, mitigating environmental hazards and, at the same time, profiting useful food with high nutritional and antioxidant properties. In this study, traditional Ecuadorian beverage (Colada) was prepared combining sweet whey, Maracuyá barley. Antioxidant properties before after in vitro digestion using INFOGEST method were determined, relationships intestinal transformations lipid protein fractions analyzed....

Two lipoxygenase isoenzymes, BBL-1 and BBL-2, were purified from broad beans. Fractionation of globulins albumins by ionic strength was preferred to the classical water extraction system ammonium sulfate fractionation as initial purification steps. From albumin fraction, BBL-2 17.6 35.7-fold, respectively, conventional gel filtration ion-exchange chromatography. The molecular weight both 97 kDa with a maximal activity around pH 5.8; however, they showed significant difference in their Km...

The biosynthesis of 2,5-dimethyl-4-hydroxy-3(2H)-furanone (Furaneol) and its methyl ether glucoside derivatives has been studied in strawberries. An vitro system was used for growing this fruit, showing that the presence incubation medium sucrose or hydroxyquinoline hemisulfate no effect on bioformation these compounds. Strawberries grown showed an increase furanone content with time, especially between second fourth days, to same extent as field-grown fruits but at a higher rate. Among...

Glycation pattern of Bowman–Birk inhibitors from soybean affects their functional properties.

The Ca2+-dependent SOS pathway has emerged as a key mechanism in the homeostasis of Na+ and K+ under saline conditions. We recently identified functionally characterized by complementation studies yeast Arabidopsis gene encoding calcineurin-interacting protein kinase tomato, SlSOS2.Citation1 also show evidences on biotechnological potential SlSOS2 conferring salt tolerance to transgenic tomato. increased salinity overexpressing plants is associated with higher sodium content stems leaves....