A5033075933- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- Genomics and Chromatin Dynamics
- Eicosanoids and Hypertension Pharmacology
- Bacteriophages and microbial interactions
- Hormonal Regulation and Hypertension
- Viral Infections and Immunology Research
- Transplantation: Methods and Outcomes
- RNA Interference and Gene Delivery
- Diffusion and Search Dynamics
- RNA Research and Splicing
- Electron Spin Resonance Studies
- Photosynthetic Processes and Mechanisms
- Organ Transplantation Techniques and Outcomes
- Alcohol Consumption and Health Effects
- Advanced Fluorescence Microscopy Techniques
- Virus-based gene therapy research
- Apelin-related biomedical research
- Renal Transplantation Outcomes and Treatments
Thermo Fisher Scientific (United States)
2024
University of California, Los Angeles
2016-2022
Oregon Health & Science University
2007-2008
Toho University
2008
Cytochrome P450 epoxygenase metabolizes arachidonic acid to epoxyeicosatrienoic acids (EETs). EETs are produced in the brain and perform important biological functions, including vasodilation neuroprotection. However, rapidly metabolized via soluble epoxide hydrolase (sEH) dihydroxyeicosatrienoic (DHETs). We tested hypothesis that sEH gene deletion is protective against focal cerebral ischemia through enhanced collateral blood flow.sEH knockout (sEHKO) mice with without antagonist 14, 15...
Significance Transcription initiation by RNA polymerase (RNAP) is a highly regulated rate-limiting step in many genes and involves numerous intermediate states that remain incompletely understood. Here, we report the characterization of previously hypothesized slow pathway involving RNAP backtracking pausing. This backtracked paused state observed when all nucleoside triphosphates (NTPs) are present at physiologically relevant concentrations, but becomes more prevalent with unbalanced NTP...
Previous works have reported significant effects of macromolecular crowding on the structure and behavior biomolecules. The crowded intracellular environment, in contrast to vitro buffer solutions, likely imparts similar enzyme serving as gatekeeper for genome, RNA polymerase (RNAP), is among most regulated enzymes. Although it was previously demonstrated that affects association RNAP DNA, not much known about how acts late initiation promoter clearance steps, which are considered be...
Abstract Initiation is a highly regulated, rate-limiting step in transcription. We employed series of approaches to examine the kinetics RNA polymerase (RNAP) transcription initiation greater detail. Quenched assays, combination with magnetic tweezer experiments and other methods, showed that, contrary expectations, RNAP exit from later stages (e.g. 7-base transcript) was markedly slower than earlier stages. Further examination implicated previously unidentified intermediate which adopted...
ABSTRACT Biological reactions in the cellular environment differ physicochemically from those performed dilute buffer solutions due to, part, slower diffusion of various components milieu, increase their chemical activities, and modulation binding affinities conformational stabilities. In vivo transcription is therefore expected to be strongly influenced by ‘crowdedness’ cell. Previous studies under macromolecular crowding conditions have focused mainly on multiple cycles RNAP-Promoter...