A5012705491- Metal Extraction and Bioleaching
- Corrosion Behavior and Inhibition
- Minerals Flotation and Separation Techniques
- Mine drainage and remediation techniques
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Hydrogen embrittlement and corrosion behaviors in metals
- Microbial Fuel Cells and Bioremediation
- Extraction and Separation Processes
- Mineral Processing and Grinding
- Radioactive element chemistry and processing
- Chromium effects and bioremediation
- Genetics, Bioinformatics, and Biomedical Research
- Protein Structure and Dynamics
- Electrochemical Analysis and Applications
- CRISPR and Genetic Engineering
- Microbial infections and disease research
- Cancer Research and Treatments
- Bat Biology and Ecology Studies
- Bacterial Genetics and Biotechnology
- Wildlife Ecology and Conservation
- Amphibian and Reptile Biology
- Enzyme Structure and Function
- Trace Elements in Health
Fundación Ciencia and Vida
2016-2025
San Sebastián University
2020-2025
Millennium Science Initiative
2018-2022
Bangor University
2016-2020
Universidad Andrés Bello
2005-2016
Millennium Institute for Integrative Biology
2005-2012
Universidad Bernardo O'Higgins
2003
National University of Comahue
2001
University of Chile
2001
Abstract Background Acidithiobacillus ferrooxidans is a major participant in consortia of microorganisms used for the industrial recovery copper (bioleaching or biomining). It chemolithoautrophic, γ-proteobacterium using energy from oxidation iron- and sulfur-containing minerals growth. thrives at extremely low pH (pH 1–2) fixes both carbon nitrogen atmosphere. solubilizes other metals rocks plays an important role nutrient metal biogeochemical cycling acid environments. The lack...
Acidithiobacillus ferrooxidans gains energy from the oxidation of ferrous iron and various reduced inorganic sulfur compounds at very acidic pH. Although an initial model for electron pathways involved in has been developed, much less is known about this microorganism. In addition, what reported both derived different A. strains, some which have not phylogenetically characterized shown to be mixed cultures. It necessary provide models within one strain order comprehend full metabolic...
Abstract Members of the genus Acidithiobacillus, now ranked within class Acidithiobacillia, are model bacteria for study chemolithotrophic energy conversion under extreme conditions. Knowledge genomic and taxonomic diversity Acidithiobacillia is still limited. Here, we present a systematic analysis nearly 100 genomes from sampled wide range habitats. Some these new others have been reclassified on basis advanced analysis, thus defining 19 lineages ranking at different levels. This work...
Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic compounds. Here we present draft genome sequence ATCC 51756 (the type strain species), which has permitted prediction genes for survival in acidic environments, including nutrient assimilation.
Acidithiobacillus thiooxidans is a mesophilic, extremely acidophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and inorganic compounds. Here we present draft genome sequence A. ATCC 19377, which has allowed identification genes for survival colonization acidic environments.
Abstract Background Normalization is a prerequisite for accurate real time PCR (qPCR) expression analysis and the validation of microarray profiling data in microbial systems. The choice use reference genes that are stably expressed across samples, experimental conditions designs key consideration interpretation gene data. Results Here, we evaluate carefully selected set derived from previous microarray-based transcriptional experiments performed on Acidithiobacillus ferrooxidans identify...
Background Acidithiobacillus caldus is a sulfur oxidizing extreme acidophile and the only known mesothermophile within Acidithiobacillales. As such, it one of preferred microbes for mineral bioprocessing at moderately high temperatures. In this study, we explore genomic diversity A. strains using combination bioinformatic experimental techniques, thus contributing first insights into elucidation species pangenome. Principal Findings Comparative sequence analysis ATCC 51756 SM-1 indicate...
The acidithiobacilli are sulfur-oxidizing acidophilic bacteria that thrive in both natural and anthropogenic low pH environments. They contribute to processes lead the generation of acid rock drainage several different geoclimatic contexts, their properties have long been harnessed for biotechnological processing minerals. Presently, genus is composed seven validated species, described between 1922 2015: Acidithiobacillus thiooxidans, A. ferrooxidans, albertensis, caldus, ferrivorans,...
Strain MG, isolated from an acidic pond sediment on the island of Milos (Greece), is proposed as a novel species ferrous iron- and sulfur-oxidizing Acidithiobacillus. Currently, four eight validated this genus oxidize iron, strain MG shares many key characteristics with these four, including capacities for catalyzing oxidative dissolution pyrite anaerobic growth via ferric iron respiration. also grows aerobically hydrogen anaerobically coupled to reduction. While 16S rRNA genes...
The γ-proteobacterium Acidithiobacillus ferrooxidans lives in extremely acidic conditions (pH 2) and, unlike most organisms, is confronted with an abundant supply of soluble iron. It also unusual that it oxidizes iron as energy source. Consequently, faces the challenging dual problems (i) maintaining intracellular homeostasis when high environmental loads and (ii) regulating use both source a metabolic micronutrient. A combined bioinformatic experimental approach was undertaken to identify...
Rubrerythrins (RBRs) are non-heme di-iron proteins belonging to the ferritin-like superfamily (FLSF). They involved in oxidative stress defense as peroxide scavengers a wide range of organisms. The vast majority RBRs, including classical forms this protein, contain C-terminal rubredoxin-like domain electron transport that is used during catalysis anaerobic conditions. Rubredoxin an ancient and large protein family short length (<100 residues) contains Fe-S center transfer. However,...
Type IV CRISPR-Cas are a distinct variety of highly derived systems that appear to have evolved from type III through the loss target-cleaving nuclease and partial deterioration large subunit effector complex. All known encoded on plasmids, integrative conjugative elements (ICEs), or prophages, thought contribute competition between these elements, although mechanistic details their function remain unknown. There is clear parallel compositions likely origin I recruited by Tn7-like...
Membrane vesicles (MVs) are envelope-derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied pathogenic microorganisms, the roles, relevance, and biotechnological potential MVs from environmental bacteria less well established. Acidithiobacillaceae family active players sulfur iron biogeochemical cycles extremely acidic environments drivers leaching mineral ores contributing to acid rock/mine drainage (ARD/AMD) industrial...
Abstract Background Iron is an essential nutrient but can be toxic at high intracellular concentrations and organisms have evolved tightly regulated mechanisms for iron uptake homeostasis. Information on management available living circumneutral pH. However, very little known about how acidophilic bacteria, especially those used industrial copper bioleaching, cope with environmental loads that 10 18 times the concentration found in pH neutral environments. This study was motivated by need to...
The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Desert, microorganisms growing this environment show varying levels of resistance/tolerance to copper, tellurium, arsenic, among others. Herein, we report genome sequence comparative genomic analysis a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic athalassohaline lake. SH31 belongs phylogenetic Group II its closest relative is S17, Argentinian...
Lake Caviahue (37° 50 ‘S and 71° 06’ W; Patagonia, Argentina) is an extreme case of a glacial, naturally acidic, aquatic environment (pH ~ 3). Knowledge the bacterial communities in water column this lake, incipient, with basal quantification bacterioplankton abundance distribution North South Basins Caviahue, described presence sulfur iron oxidizing bacteria lake sediments. The role that plays nutrient utilization recycling environment, especially phosphorus cycle, has not been studied. In...