A5027516334- RNA Research and Splicing
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Amyotrophic Lateral Sclerosis Research
- Osteoarthritis Treatment and Mechanisms
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Cell Adhesion Molecules Research
- Neurogenetic and Muscular Disorders Research
- Plant Virus Research Studies
- NF-κB Signaling Pathways
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Bone Metabolism and Diseases
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- TGF-β signaling in diseases
- Genetics and Neurodevelopmental Disorders
- Nuclear Receptors and Signaling
- Epigenetics and DNA Methylation
- Bone health and treatments
Farmingdale State College
2016-2021
Stony Brook University
2006-2021
Cold Spring Harbor Laboratory
2012-2017
New York University Langone Orthopedic Hospital
2004-2006
York University
2006
Amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) are two incurable neurodegenerative disorders that exist on a symptomological spectrum share both genetic underpinnings pathophysiological hallmarks. Functional abnormality of TAR DNA-binding protein 43 (TDP-43), an aggregation-prone RNA DNA binding protein, is observed in the vast majority familial sporadic ALS cases ~40% FTLD cases, but cascade events leading to cell death not understood. We have expressed...
Elevated expression of specific transposable elements (TEs) has been observed in several neurodegenerative disorders. TEs also can be active during normal neurogenesis. By mining a series deep sequencing datasets protein-RNA interactions and gene profiles, we uncovered extensive binding TE transcripts to TDP-43, an RNA-binding protein central amyotrophic lateral sclerosis (ALS) frontotemporal lobar degeneration (FTLD). Second, find that association between TDP-43 many its targets is reduced...
Degradative fragments of cartilage oligomeric matrix protein (COMP) have been observed in arthritic patients. The physiological enzyme(s) that degrade COMP, however, remain unknown. We performed a yeast two-hybrid screen (Y2H) to search for proteins associate with COMP identify an interaction partner might it. One using the epidermal growth factor (EGF) domain as bait led discovery ADAMTS-7. Rat ADAMTS-7 is composed 1595 amino acids, and this exhibits higher expression musculoskeletal...
Evidence is rapidly mounting that transposable element (TE) expression and replication may impact biology more widely than previously thought. This includes potential effects on normal physiology of somatic tissues dysfunctional impacts in diseases associated with aging, such as cancer neurodegeneration. Investigation the biological mobile elements cells will be greatly facilitated by use donor are engineered to report de novo events vivo. In multicellular organisms, reporter constructs...
The differentiation of uncommitted mesenchymal cells into osteoblasts is a fundamental molecular event governing both embryonic development and bone repair. morphogenetic proteins (BMPs) are important regulators this process; they function by binding to cell surface receptors signaling means Smad proteins. Core factor α-1 (Cbfa1), member the runt family transcription factors, an essential transcriptional regulator osteoblast formation, process positively or negatively regulated variety...
A hallmark of genes that are subject to developmental regulation transcriptional elongation is association the negative factor NELF with paused RNA polymerase complex. Here we use a combination biochemical and genetic experiments investigate in vivo function Drosophila embryo. associates different gene promoter regions correlation II (Pol II) initial activation expression during early stages embryogenesis. Genetic reveal maternally provided required for activation, rather than repression...
The molecular mechanisms by which mesenchymal cells differentiate into chondrocytes are poorly understood. cartilage oligomeric matrix protein gene (COMP) encodes a noncollagenous extracellular whose expression pattern correlates with chondrocyte differentiation and arthritis. We have used the COMP promoter as model to identify regulatory sequences necessary for chondrocyte-specific cell type-specific proteins that bind these sequences. previously cloned 1.9 kilobases of 5(') flanking...
ABSTRACT Amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) are two incurable neurodegenerative disorders that exist on a symptomological spectrum share both genetic underpinnings pathophysiological hallmarks. Functional abnormality of TAR DNA-binding protein 43 (TDP-43), an aggregation-prone RNA DNA binding protein, is observed in the vast majority familial sporadic ALS cases ∼40% FTLD cases, but cascade events leading to cell death not understood. We have...
Abstract Evidence is rapidly mounting that transposable element expression and replication may impact biology more widely than previously thought. This includes potential effects on normal physiology of somatic tissues dysfunctional impacts in diseases associated with aging such as cancer neurodegeneration. Investigation the biological mobile elements cells will be greatly facilitated by use donor are engineered to report de novo events vivo. In multicellular organisms, successful reporters...
This work investigates the role of DNA binding by Runt in regulating sloppy paired 1 (slp1) gene and particular two distinct cis-regulatory elements that mediate regulation other pair-rule transcription factors during Drosophila segmentation. We find a DNA-binding-defective form is ineffective at repressing both distal (DESE) proximal (PESE) early stripe slp1 also compromised for DESE-dependent activation. The function Runt-binding sites DESE further investigated using site-specific...