A5042057438- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Gene expression and cancer classification
- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Estrogen and related hormone effects
- Biosensors and Analytical Detection
- Force Microscopy Techniques and Applications
- Near-Field Optical Microscopy
- Epigenetics and DNA Methylation
- Cellular Mechanics and Interactions
- Diffusion and Search Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Chromosomal and Genetic Variations
- T-cell and B-cell Immunology
- Photoacoustic and Ultrasonic Imaging
- Advanced Biosensing Techniques and Applications
Institut Curie
2021-2022
Université Paris Sciences et Lettres
2021-2022
Sorbonne Université
2021-2022
Centre National de la Recherche Scientifique
2021-2022
National Institutes of Health
2022
Dynamique du noyau
2021-2022
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2020
Leiden University
2014-2019
Our understanding of the physical principles organizing genome in nucleus is limited by lack tools to directly exert and measure forces on interphase chromosomes vivo probe their material nature. Here, we introduce an approach actively manipulate a genomic locus using controlled magnetic inside living human cell. We observed viscoelastic displacements over micrometers within minutes response near-piconewton forces, which are consistent with Rouse polymer model. results highlight fluidity...
Recent advances in live cell imaging have provided a wealth of data on the dynamics transcription factors. However, consistent quantitative description these dynamics, explaining how factors find their target sequences vast amount DNA inside nucleus, is still lacking. In present study, we combined two methods, single-molecule microscopy and fluorescence recovery after photobleaching, to determine mobility pattern glucocorticoid receptor (GR) mineralocorticoid (MR), ligand-activated For...
Article18 September 2020Open Access Source DataTransparent process A genetic memory initiates the epigenetic loop necessary to preserve centromere position Sebastian Hoffmann Institut Curie, CNRS, UMR 144, PSL Research University, Paris, France Search for more papers by this author Helena M Izquierdo INSERM U932, Riccardo Gamba Florian Chardon Marie Dumont Veer Keizer Solène Hervé Shannon McNulty Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC,...
Transcription factor mobility is a determining in the regulation of gene expression. Here, we have studied intranuclear dynamics glucocorticoid receptor (GR) using fluorescence recovery after photobleaching and single-molecule microscopy. First described dynamic states which GR occurs. Subsequently analyzed transitions between these continuous time Markov chain model, functionally investigated by making specific mutations DNA-binding domain. This analysis revealed that diffuses freely...
Abstract Our understanding of the physical principles organizing genome in nucleus is limited by lack tools to directly exert and measure forces on interphase chromosomes vivo probe their material nature. Here, we present a novel approach actively manipulate genomic locus using controlled magnetic inside living human cell. We observe viscoelastic displacements over microns within minutes response near-picoNewton forces, which are well captured Rouse polymer model. results highlight fluidity...
Single-molecule imaging of proteins in a 2D environment like membranes has been frequently used to extract diffusive properties multiple fractions receptors. In 3D the apparent however change with observation time due movements molecules out depth-of-field microscope. Here we developed mathematical framework that allowed us correct for fraction size limited detection volume single-molecule imaging. We applied our findings on mobility activated glucocorticoid receptors cell nucleus, and found...