A5012142979- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Muscle Physiology and Disorders
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Cardiac electrophysiology and arrhythmias
- Cardiomyopathy and Myosin Studies
- Neurobiology and Insect Physiology Research
- Exercise and Physiological Responses
- Muscle activation and electromyography studies
- Ion Channels and Receptors
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Insect and Pesticide Research
- Antioxidant Activity and Oxidative Stress
- Cannabis and Cannabinoid Research
- Cellular transport and secretion
- Insect and Arachnid Ecology and Behavior
- Lipoproteins and Cardiovascular Health
- Sleep and Wakefulness Research
- Spaceflight effects on biology
- Photoacoustic and Ultrasonic Imaging
- Fungal and yeast genetics research
- Calcium signaling and nucleotide metabolism
- Nicotinic Acetylcholine Receptors Study
University of Debrecen
2016-2025
Research Network (United States)
2023
Hungarian Research Network
2023
Vrije Universiteit Amsterdam
2005
University of Bern
2004
University of Cincinnati
2004
Hungarian Academy of Sciences
1999-2000
1 The contractile properties and ATPase activity of skinned human skeletal muscle fibres from vastus lateralis were examined. Fibre types resolved single fibre segments by SDS-polyacrylamide gel electrophoresis. was determined enzymatic coupling ATP resynthesis to the oxidation NADH. 2 partitioning into (a) calcium-activated due actomyosin (AM) interaction, (b) sarcoplasmic reticular (SR) calcium pump, (c) basal (calcium independent) investigated comparing utilization before after exposure...
In cardiac muscle Ca 2+ -induced release (CICR) from the sarcoplasmic reticulum (SR) is initiated by influx via L-type channels. At present, mechanisms underlying termination of SR release, which are required to ensure stable excitation-contraction coupling cycles, not precisely known. However, same mechanism leading refractoriness could also be responsible for CICR. To examine we analyzed Na + -Ca exchange currents reflecting cytosolic signals induced UV-laser flash-photolysis caged . Pairs...
Triadin is a multiple proteins family, some isoforms being involved in muscle excitation-contraction coupling, and having still unknown functions. To obtain clues on triadin functions, we engineered knock-out mouse line characterized the physiological effect of ablation skeletal function. These mice presented reduced strength, which seemed not to alter their survival has been present work. We first checked these expression level different calcium homeostasis observed fast muscles an increase...
The effects of the muscle relaxant dantrolene on steps excitation-contraction coupling were studied fast twitch muscles rodents. To identify site action drug, single fibers for voltage-clamp measurements, heavy SR vesicles calcium efflux studies and solubilized release channels/RYRs lipid bilayer isolated. Using double Vaseline-gap or silicone-clamp technique, was found to suppress depolarization-induced elevation in intracellular concentration ([Ca2+]i) by inhibiting from SR. suppression...
Skeletal muscle excitation-contraction (EC) coupling is independent of calcium influx. In fact alternative splicing the voltage-gated channel CaV1.1 actively suppresses currents in mature muscle. Whether this necessary for normal development and function not known. However, defects causing aberrant expression calcium-conducting developmental CaV1.1e splice variant correlate with weakness myotonic dystrophy. Here we deleted exon 29 mice. These mice displayed overall motor performance,...
Abstract During aging reduction in muscle mass (sarcopenia) and decrease physical activity lead to partial loss of force increased fatigability. Deficiency the essential trace element selenium might augment these symptoms as it can cause pain, fatigue, proximal weakness. Average voluntary daily running, maximal twitch tetanic force, calcium release from sarcoplasmic reticulum (SR) decreased while reactive oxygen species (ROS) production associated with contractions aged – 22-month-old...
As an essential trace element selenium plays a significant role in many physiological functions of the organs. It is found within muscles as selenocystein selenoprotein N, which involved redox-modulated calcium homeostasis and protection against oxidative stress.The effects two different compounds (selenate NanoSe 0.5 5 ppm concentration for weeks) on muscle properties mice were examined by measuring vivo performance, vitro force soleus (SOL) extensor digitorum longus (EDL) changes...
The endocannabinoid system (ECS) regulates and controls many of our most critical bodily functions including healthy muscles however, its potential role(s) in physiological skeletal muscle function disorders are unclear. Earlier using the Cre/LoxP we generated characterized a tamoxifen-inducible skel-etal muscle-specific CB1R knockdown (skmCB1-KD) mouse model. Here, sought to elucidate mechanisms underlying reduced force generation. To address this, measured calcium movements following...
We have previously shown that neuromodulatory actions on astrocytes can elicit metabotropic glutamate- and N-methyl-D-aspartate receptor-dependent tonic changes in excitability the mesopontine region. Although vitro experiments explored robust effects, vivo significance of our findings remained unknown. In this project, chronic chemogenetic activation its movement, circadian activity, acoustic startle spatial memory were tested. The control group young adult male mice where expressed only...
Isometric force production and ATPase activity were determined simultaneously in single human skeletal muscle fibers (n = 97) from five healthy volunteers nine patients with chronic heart failure (CHF) at 20°C. The permeabilized by means of Triton X-100 (1% vol/vol). was enzymatic coupling ATP resynthesis to the oxidation NADH. Calcium-activated actomyosin (AM) obtained subtracting measured relaxing (pCa 9) solutions that maximally activating 4.4) solutions. Fiber type on basis myosin heavy...
Key points Marijuana was found to cause muscle weakness, although the exact regulatory role of its receptors (CB1 cannabinoid receptor; CB1R) in excitation–contraction coupling (ECC) mammalian skeletal remains unknown. We that CB1R activation or knockout did not affect force directly, whereas decreased Ca 2+ ‐sensitivity contractile apparatus and made fibres more prone fatigue. demonstrate CB1Rs are connected inositol 1,4,5‐trisphosphate pathway either myotubes adult fibres. By contrast,...
Single muscle fibres were dissociated enzymatically from the extensor digitorum longus and communis muscles of rats guinea‐pigs. The mounted into a double Vaseline gap experimental chamber events in excitation–contraction coupling studied under voltage clamp conditions. dependence intramembrane charge movement followed two‐state Boltzmann distribution with maximal available 26.1 ± 1.5 1.3 nC μF −1 , mid‐point –35.1 5.0 –42.2 1.2 mV steepness 16.7 2.2 17.0 1.9 (means s.e.m. n = 7 4)...
Key points Hypermuscularity associated with naturally occurring mutations in the myostatin gene as found Compact mice results increased muscle mass but reduced specific force. The calcium sensitivity of contractile apparatus assessed on chemically skinned skeletal fibres under isometric conditions is not altered these animals. While resting concentration remains unaffected, depolarization‐evoked increases intracellular are suppressed. Spontaneous release events from sarcoplasmic reticulum...
Today septins are considered as the fourth component of cytoskeleton, with Septin7 isoform playing a critical role in formation higher-order structures. While its importance has already been confirmed several intracellular processes different organs, very little is known about skeletal muscle. Here, using conditional knockdown (KD) mouse model, C2C12 cell line, and enzymatically isolated adult muscle fibers, organization localization septin filaments revealed, an ontogenesis-dependent...
The remodelling of the extracellular matrix plays an important role in skeletal muscle development and regeneration. Syndecan-4 is a cell surface proteoglycan crucial for differentiation. Syndecan-4−/− mice have been reported to be unable regenerate following damage. To investigate consequences decreased expression Syndecan-4, we studied vivo vitro performance excitation–contraction coupling machinery young aged Syndecan-4+/− (SDC4) mice. In grip force was significantly as well average...
Key points Dynamin 2 is a ubiquitously expressed protein involved in membrane trafficking processes. Mutations the gene encoding dynamin are responsible for congenital myopathy associated with centrally located nuclei muscle fibres. Using fibres from mouse model of most common mutation this disease humans, we tested whether altered Ca 2+ signalling and excitation–contraction coupling contribute to weakness. The plasma network that carries electrical excitation moderately perturbed diseased...
PAF, a small antifungal protein from Penicillium chrysogenum, inhibits the growth of several pathogenic filamentous fungi, including members Aspergillus genus. PAF has been proven to have no toxic effects in vivo mice by intranasal application. To test its efficacy against invasive pulmonary aspergillosis (IPA), experiments were carried out suffering IPA. Adult immunosuppressed and then infected with fumigatus. After stable infection, animals inoculated intranasally at concentration 2.7...
The 95 kDa triadin (Trisk 95), an integral protein of the sarcoplasmic reticular membrane in skeletal muscle, interacts with both ryanodine receptor (RyR) and calsequestrin. While its role regulation calcium homeostasis has been extensively studied, data are not available on whether overexpression or interference expression Trisk would affect sparks localized events release (LCRE). In present study LCRE transients were studied using laser scanning confocal microscopy C2C12 cells primary...