A5101626506- Dental Trauma and Treatments
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Congenital heart defects research
- ATP Synthase and ATPases Research
- Peptidase Inhibition and Analysis
- Force Microscopy Techniques and Applications
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cardiomyopathy and Myosin Studies
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Mesenchymal stem cell research
Shenyang Agricultural University
2022
Central South University
2010-2021
Hunan Cancer Hospital
2021
South China University of Technology
2020
Guangzhou Medical University
2020
Guangzhou First People's Hospital
2020
Massachusetts General Hospital
2007
The aim of this study was to inhibit adipogenic differentiation by transfecting two growth factors, platelet-derived factor (PDGF-BB) and bone morphogenic protein 2 (BMP-2), into modified rat marrow mesenchymal stem cells (rBMSCs) then compounded with platelet-rich plasma (PRP). To achieve rBMSCs, the osteoporosis model rats established, rBMSCs from were isolated identified. Co-transfection PDGF-BB-GFP BMP-2 detection PDGF-BB/BMP-2 expression in transfected BMSCs assessed qRT-PCR western...
Objective To investigate the role of Rho in SiO2 induced α-SMA expression human bronchial epithelial cells ( HBECs) . Methods HBECs were cultured and stimulated with SiO2. Immunocytochemistry Western blot used to detect α-SMA. The activity was determined by GST pull down assay. In prevention experiment,SiO2-stimulated incubated inhibitor Y27632,and examined blot. Results With SiO2( 0 - 300 μg/mL) treatment,the increased gradually,and 200 μg/mL led highest which 5. 09 ± 1. 98) times control...
The aim of this study was to improve osteoblast function by incorporating rat bone marrow stromal stem cells (rBMSCs) overexpressing platelet-derived growth factor (PDGF-BB) and morphogenic protein (BMP-2) into platelet-rich plasma (PRP) gels. rBMSCs were isolated, cultured, identified. subsequently co-transfected with two recombinant adenoviruses delivering PDGF-BB-GFP BMP-2-GFP. PDGF-BB BMP-2 expression levels in transduced BMSCs detected, a post-transfection analysis the osteogenic...
In the present study, we report that a GFP fusion tag facilitated soluble expression of pea actin isoform (PEAc1) in E. coli. To investigate influence on PEAc1 structure and activity, PEAc1, His-tagged (His-PEAc1), (His-GFP), with (His-PEAc1-GFP) were expressed SDS-PAGE western blot analyses reveal solubility His-PEAc1-GFP was higher than His-PEAc1. The His-GFP proteins purified from supernatant cell homogenate Ni-affinity column, His-PEAc1 inclusion bodies. CD spectrum analysis four...