A5047481335- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Diet, Metabolism, and Disease
- Biofuel production and bioconversion
- Food composition and properties
- Plant nutrient uptake and metabolism
- Microbial Metabolic Engineering and Bioproduction
- Seaweed-derived Bioactive Compounds
- Enzyme Catalysis and Immobilization
- Probiotics and Fermented Foods
- Connexins and lens biology
- Advanced Glycation End Products research
- Biochemical effects in animals
- Polysaccharides and Plant Cell Walls
- Enzyme Structure and Function
- Protein Hydrolysis and Bioactive Peptides
- Legume Nitrogen Fixing Symbiosis
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Algal biology and biofuel production
- Phase Equilibria and Thermodynamics
- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- Botanical Research and Applications
Jiangnan University
2016-2025
State Key Laboratory of Food Science and Technology
2015-2024
Yixing People's Hospital
2023
Jiangsu University
2023
The noncharacterized protein ACL75304 encoded by the gene Ccel_0941 from Clostridium cellulolyticum H10 (ATCC 35319), previously proposed as xylose isomerase domain TIM barrel, was cloned and expressed in Escherichia coli . enzyme purified nickel-affinity chromatography with electrophoretic homogeneity then characterized d-psicose 3-epimerase. strictly metal-dependent showed a maximal activity presence of Co(2+). optimum pH temperature for were 55 °C 8.0. half-lives at 60 6.8 h 10 min when...
d-Psicose is a highly valuable rare sugar because of its excellent physiological properties and commercial potential. 3-epimerase (DPEase) the key enzyme catalyzing isomerization d-fructose to d-psicose. However, poor thermostability low catalytic efficiency are serious constraints on industrial application. To address these issues, site-directed mutagenesis Tyr68 Gly109 Clostridium bolteae DPEase was performed. Compared with wild-type enzyme, Y68I variant displayed highest substrate-binding...
The laver crude polysaccharides were extracted, purified, and subsequently degraded using H<sub>2</sub>O<sub>2</sub>. One low-molecular-weight polysaccharide PD-1 showing the highest inhibition activity against α-amylase might be used as a novel agent for T2DM management.
Difructose anhydride I (DFA-I) can be produced from inulin, with DFA-I-forming inulin fructotransferase (IFTase-I). However, the metabolism of through DFA-I remains unclear. To clarify this pathway, several genes enzymes related to pathway in genome
Difructose anhydride I (DFA-I) is a prospective prebiotic whose physiological functions have yet to be elucidated. This study aimed delineate the role of DFA-I, highlighting its nondigestibility and subsequent fermentation by human fecal microbiota. Our findings underscore potential DFA-I enhance production short-chain fatty acids (SCFAs), notably butyric acid (7.69 mM after 48 h period), lower pH. The augmentation in SCFA concentration decrease pH coincide with proliferation beneficial...
Lactosucrose, a rare trisaccharide formed from sucrose and lactose by enzymatic transglycosylation, is type of indigestible carbohydrate with good prebiotic effect. In this study, lactosucrose biosynthesis was efficiently carried out purified levansucrase Leuconostoc mesenteroides B-512. The target gene cloned expressed in Escherichia coli, the recombinant enzyme to homogeneity nickel affinity gel filtration chromatography. effects pH, temperature, substrate concentration, ratio, amount on...
Melibiose, which is an important reducing disaccharide formed by α-1,6 linkage between galactose and glucose, has been proven to have beneficial applications in both medicine agriculture. In this study, a characterized levansucrase from Leuconostoc mesenteroides B-512 FMC was further used study the bioproduction of melibiose raffinose. The reaction conditions were optimized for synthesis. optimal pH, temperature, substrate concentration, ratio substrates, units enzymes determined as pH 6.0,...