A5102731466- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- Transgenic Plants and Applications
- Peptidase Inhibition and Analysis
- Fungal and yeast genetics research
- Oral microbiology and periodontitis research
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Dental Health and Care Utilization
- Biochemical and Molecular Research
- Biosimilars and Bioanalytical Methods
- Virology and Viral Diseases
- Cancer therapeutics and mechanisms
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Viral Infections and Immunology Research
- Trypanosoma species research and implications
- HIV/AIDS oral health manifestations
- Antifungal resistance and susceptibility
- Antibiotic Resistance in Bacteria
- Advanced Biosensing Techniques and Applications
Khulna University
2022
University of Saskatchewan
2012-2020
Royal University Hospital
2018-2020
University of Toronto
2018
Construction of antibody-based, molecular-targeted optical imaging probes requires the labeling an antibody with a fluorophore. The most common method for doing this involves non-specifically conjugating fluorophore to antibody, resulting in poorly defined, heterogeneous that often have suboptimal vivo behavior. We tested new strategy site-specific label antibody-based using SpyCatcher/SpyTag protein ligase system.We used system site specifically nimotuzumab, anti-EGFR and anti-HER3 diabody....
Abstract Efforts to engineer recombinant antibodies for specific diagnostic and therapy applications are time consuming expensive, as each new antibody needs be optimized expression, stability, bio‐distribution, pharmacokinetics. We have developed a way construct antibody‐like “devices” by using bottom‐up approach build them from well‐behaved discrete domains or “parts”. Studies on structure function identified constant variable with functions that can expressed in isolation. used the...
Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties. Multermerization is one strategy that has been used produce two or more antigen binding sites. Multimerization enhances the functional affinity (avidity) and can be optimize size Most multimerization involve genetically fusing non-covalently linking fragments using oligomerization domains. Recent studies have defined guidelines for producing optimal...
Synthetic antibody libraries have been used to generate antibodies with favorable biophysical and pharmacological properties. Here, we describe the design, construction, validation of a phage-displayed antigen-binding fragment (Fab) library built on modified trastuzumab framework four fixed two diversified complementarity-determining regions (CDRs). CDRs L1, L2, H1, H2 were preserve most commonly observed "canonical" CDR conformation preferred by Fab framework. The diversity was engineered...
Abstract Streptococcus mutans and sobrinus are the main causative agents of human dental caries. Current strategies for treating caries costly do not completely eradicate them completely. Passive immunization using nonhuman antibodies against Streptococcal surface antigens has shown success in trials, however they often invoke immune reactions. We used phage display to generate antigen-binding fragments (Fabs) S . These Fabs were readily expressed E coli bound inhibited sucrose-induced...
Deletion or repression of Aspergillus nidulans ugmA (AnugmA), involved in galactofuranose biosynthesis, impairs growth and increases sensitivity to Caspofungin, a β-1,3-glucan synthesis antagonist. The A. fumigatus UgmA (AfUgmA) crystal structure has been determined. From that study, AfUgmA mutants with altered enzyme activity were transformed into AnugmA▵ assess their effect on wall composition nidulans. complemented (AnugmA::wild type AfugmA) strain had wild phenotype, indicating these...
To develop imaging and therapeutic agents, antibodies are often conjugated randomly to a chelator/radioisotope or drug using primary amine (NH2) of lysine sulfhydryl (SH) cysteine. Random conjugation NH2 SH groups can require extreme conditions may affect target recognition/binding must therefore be tested. In the present study, nimotuzumab was site-specifically labeled ∆N-SpyCatcher/SpyTag with different chelators radiometals. Nimotuzumab is well-tolerated anti-EGFR antibody low skin...
Saccharomyces cerevisiae Hansen GAL1 (galactokinase) generates galactose-1-phosphate; GAL7 (galactose-1-phosphate uridylyltransferase) transfers UDP between galactose or glucose and their respective sugar-1-phosphate conjugates, both are essential on galactose. Aspergillus nidulans ANID_04957 has 41% amino acid sequence identity with GAL1; ANID_06182 50% GAL7. The names GalE GalD consistent prior studies. Complemented galDΔ:ScGAL7 galEΔ:ScGAL1 strains had wild-type phenotype, demonstrating...
The cover feature picture shows a molecular toolkit for constructing synthetic antibodies. A new modular approach to post-translationally assemble antibody-like “devices” from well-behaved antibody domains or “parts” by using the Spytag/SpyCatcher protein ligase system is demonstrated. This allows parts be assembled in configurations that are not possible with genetic engineering approaches. We predict this will create optimized devices less time and at reduced costs. More information can...
The level of antibody titer against infectious bursal disease (IBD) in commercial chickens was determined using comparative sero evaluation through indirect ELISA test method. One hundred 1 day old were collected from a hatchery and they divided into two treatment groups named as ‘treatment group’ (flock 1) ‘control 2). Serum samples the flocks randomly four times on 1, 8, 16 28. examined to quantify A variation observed among different flocks. Mean titers found at 8686.4 9304.07 flock 2...