A5073675121- Gut microbiota and health
- Inflammatory Bowel Disease
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal motility and disorders
- Oral and gingival health research
- Enzyme Structure and Function
- Innovative Microfluidic and Catalytic Techniques Innovation
- Digestive system and related health
- Protein Structure and Dynamics
- Computational Drug Discovery Methods
- Oral microbiology and periodontitis research
- Scientific Computing and Data Management
- Probiotics and Fermented Foods
- Neonatal and Maternal Infections
- Pancreatitis Pathology and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Diet and metabolism studies
- Pesticide and Herbicide Environmental Studies
- Weed Control and Herbicide Applications
- Infant Health and Development
- Pediatric Hepatobiliary Diseases and Treatments
- Immune responses and vaccinations
- Food Allergy and Anaphylaxis Research
- Drug Transport and Resistance Mechanisms
- 3D Printing in Biomedical Research
Microbiologie de l’alimentation au service de la santé
2018-2024
AgroParisTech
2021-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2024
Université Paris-Saclay
2021-2024
European Molecular Biology Laboratory
2013-2022
Unit of Virus Host Cell Interactions
2015-2016
Université Grenoble Alpes
2015-2016
Centre National de la Recherche Scientifique
2015-2016
University of Reading
2016
Institut de Pharmacologie et de Biologie Structurale
2016
Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting processing largely depend on manual operations. Here, a series new methods are presented based low X-ray-background film as support photoablation laser that enable automation major operations required preparation crystals diffraction experiments. In this approach, controlled removal mother liquor before mounting...
The immediate early gene product Arc (activity-regulated cytoskeleton-associated protein) is posited as a master regulator of long-term synaptic plasticity and memory. However, the physicochemical structural properties have not been elucidated. In present study, we expressed purified recombinant human (hArc) performed first biochemical biophysical analysis hArc's structure stability. Limited proteolysis assays MS indicate that hArc has two major domains on either side central more disordered...
In eukaryotes, the serpins constitute a wide family of protease inhibitors regulating many physiological pathways. Many reports stressed key role in several human physiopathologies including mainly inflammatory bowel diseases. this context, eukaryotic were largely studied and their use to limit inflammation was reported. comparison that, bacterial those from gut microbiota remain poorly studied. The two genes encoding for putative bacterium Eubacterium sireaum, display low sequence...
EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography services to users worldwide. The HTX lab has strong focus in development of new methods macromolecular crystallography. Through combination crystallization platform, CrystalDirect technology for fully automated crystal mounting and cryocooling CRIMS software we have developed pipelines that can be remotely operated over internet. These include...
Chronic digestive disorders are of increasing incidence worldwide with expensive treatments and no available cure. Available therapeutic schemes mainly rely on symptom relief, large degrees variability in patients' response to such treatments, underlining the need for new strategies. There strong indications that gut microbiota's contribution seems be a key modulator disease activity treatment responses. Hence, efforts have been devoted understanding host–microbe interactions mechanisms...
In cats and humans, several physiological environmental factors have been shown to alter the gut microbiota of healthy individuals. Cats share diseases with humans such as inflammatory bowel low-grade intestinal T-cell lymphoma. The physiopathology these chronic enteropathies is poorly understood but may involve disequilibrium composition disruption normal microbiome activity profiles. These disorders are increasingly diagnosed in feline species due improved medicalization easier access...
Understanding the mode and site of action a herbicide is key for its efficient development, evaluation toxicological risk, weed control resistance management. Recently, (MoA) cinmethylin was identified in lipid biosynthesis with acyl-ACP thioesterase (FAT) as (SoA). Cinmethylin registered selective use cereal crops grass weeds 2020.Here, we present high-resolution co-crystal structure FAT complex cumyluron by high throughput crystallization screen. We show binding to inhibition cumyluron....
Serine proteases are involved in many biological processes and associated with irritable bowel syndrome (IBS) pathology. An increase serine protease activity has been widely reported IBS patients. While most of the studies focused on host proteases, contribution microbial poorly studied. In present study, we report analysis proteolytic activities fecal samples from first Tunisian cohort IBS-M patients healthy individuals. We demonstrated, for time, that metalloproteases were fourfold higher...
EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography services to users worldwide. The HTX lab has strong focus in development of new methods macromolecular crystallography. Through combination crystallization platform, CrystalDirect technology for fully automated crystal mounting and cryocooling CRIMS software we have developed pipelines that can be remotely operated over internet. These include...
Abstract Background The gut microbiota and derived metabolites play a key role in regulating host physiology. Diet is identified as regulatory factor of the composition and, potentially, subsequent functionalities. Demonstrating diet may be complex most human studies are cross-sectional dietary intervention often accompanied by hygienic changes. objective present study was to investigate impact an industrial on modulation targeted functionalities using canine “natural” model. Results We...
Increased protease activity has been linked to the pathogenesis of IBD. While most studies have focusing on host proteases in gut inflammation, it remains unclear how address potential contribution their bacterial counterparts. In present study, we report a functional characterization newly identified serine protease, SP-1, from human microbiota. The repertoire
Le microbiote intestinal constitue un organe à part entière qui contribue significativement au développement et maintien de la physiologie l’hôte. Plusieurs modalités dialogue microbiote-hôte sont rapportées comme production d’acides gras chaine courte, le métabolisme des sels biliaires, d’indoles l’activité protéolytique. Les entéropathies inflammatoires chroniques (EIC) l’humain caractérisées par une activité protéolytique exacerbée essentiellement liée protéases sérine (PS). De plus, il a...