A5062372955- Computational Drug Discovery Methods
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- HIV/AIDS drug development and treatment
- Plant biochemistry and biosynthesis
- RNA Research and Splicing
- RNA modifications and cancer
- Biochemical and Molecular Research
- Crystallography and molecular interactions
- Free Radicals and Antioxidants
- DNA and Nucleic Acid Chemistry
- Molecular Sensors and Ion Detection
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Synthesis and Characterization of Heterocyclic Compounds
- Mass Spectrometry Techniques and Applications
- Lipid metabolism and biosynthesis
- Trace Elements in Health
- Crystallization and Solubility Studies
- ATP Synthase and ATPases Research
- Spectroscopy and Quantum Chemical Studies
- Protein Kinase Regulation and GTPase Signaling
- Synthesis and biological activity
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2016-2025
Polish Academy of Sciences
2006-2023
Polish Academy of Learning
2022
In-Q-Tel
2011
University of Wrocław
2010
Central Institute for Labour Protection
2007-2010
Institute of Physical Chemistry
1998-2010
University of Warsaw
2000-2010
National Institute of Chemistry
2007
Medical University of Białystok
2003
Research Article18 March 2019Open Access Source DataTransparent process Inhibition of proteasome rescues a pathogenic variant respiratory chain assembly factor COA7 Karthik Mohanraj Laboratory Mitochondrial Biogenesis, Centre New Technologies, University Warsaw, Poland ReMedy International Agenda Unit, Institute Molecular and Cell Biology, Search for more papers by this author Michal Wasilewski Corresponding Author [email protected] orcid.org/0000-0002-4890-5103 Cristiane Benincá MRC Biology...
Abstract The nine identified human homologues of E . coli AlkB 2-oxoglutarate (2OG) and Fe(II)-dependent dioxygenase, ALKBH1-8 FTO, display different substrate specificities diverse biological functions. Here we discovered the combined overexpression members ALKBH family in head neck squamous cell carcinomas (HNSCC). We found direct correlation ALKBH3 FTO expression with primary HNSCC tumor size. observed unidentified thus far cytoplasmic localization ALKBH2 5 HNSCC, suggesting abnormal...
Plant isoprenoids are derived from two biosynthetic pathways, the cytoplasmic mevalonate (MVA) and plastidial methylerythritol phosphate (MEP) pathway. In this study their respective contributions toward formation of dolichols in Coluria geoides hairy root culture were estimated using vivo labeling with (13)C-labeled glucose as a general precursor. NMR mass spectrometry showed that both MVA MEP pathways sources isopentenyl diphosphate incorporated into polyisoprenoid chains. The involvement...
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is a positive-strand RNA virus that causes severe syndrome in humans, which now referred to as coronavirus disease 2019 (COVID-19). Since December 2019, the new pathogen has rapidly spread globally, with over 65 million cases reported beginning of 2020, including 1.5 deaths. Unfortunately, currently, there no specific and effective treatment for COVID-19. As SARS-CoV-2 relies on its spike proteins (S) bind host cell-surface...
Tn7 is a bacterial transposon with relatives containing element-encoded CRISPR-Cas systems mediating RNA-guided insertion. Here, we present the 2.7 Å cryoelectron microscopy structure of prototypic transposase TnsB interacting end DNA. When interacts across repeating binding sites, it adopts beads-on-a-string architecture, where DNA-binding and catalytic domains are arranged in tiled intertwined fashion. The form few base-specific contacts leading to preference that requires multiple weakly...
Isoprenoids, including dolichols (Dols) and polyprenols (Prens), are ubiquitous components of eukaryotic cells. In plant cells, there two pathways that produce precursors utilized for isoprenoid biosynthesis: the mevalonate (MVA) pathway methylerythritol phosphate (MEP) pathway. this work, contribution these to biosynthesis Prens Dols was addressed using an in planta experimental model. Treatment plants with pathway-specific inhibitors analysis effects various light conditions indicated...
Previously we demonstrated for several examples that peptides having a general internal sequence R(N)-Yaa-Ser/Thr-Xaa-His-Zaa-R(C) (Yaa = Glu or Ala, Xaa Ala His, Zaa Lys, R(N) and R(C) any N- C-terminal amino acid sequence) were hydrolyzed specifically at the Yaa-Ser/Thr peptide bond in presence of Ni(II) ions alkaline pH (Krezel, A., Mylonas, M., Kopera, E. Bal, Acta Biochim. Polon. 2006, 53, 721-727 references therein). Hereby report synthesis combinatorial library...
Recently we screened a combinatorial library of R1-(Ser/Thr)-Xaa-His-Zaa-R2 peptides (Xaa = 17 common α-amino acids, except Asp, Glu, and Cys; Zaa =19 R1 CH3CO-Gly-Ala, R2 Lys-Phe-Leu-NH2) established criteria for selecting Ser/Thr, Xaa, substitutions optimal specific R1-Ser/Thr peptide bond hydrolysis in the presence Ni(II) ions (Krȩżel, A.; Kopera, E.; Protas, A. M.; Poznański, J.; Wysłouch-Cieszyńska, Bal, W. J. Am. Chem. Soc. 2010, 132, 3355−3366). The screening results were confirmed by...
Noonan syndrome (NS) is a common developmental disorder presenting with dysmorphic craniofacial features, heart defects, and short stature. It belongs to the group of RASopathies caused by germline mutations in genes encoding proteins involved RAS/MAPK signaling pathway. Although nine are known cause NS, approximately 30% cases still have unexplained etiology. To identify new causative genes, 42 patients clinical diagnosis who had negative results on Sanger sequencing PTPN11 , SOS1 RAF1 (the...
Abstract Mounting evidence indicates that MS analysis of the human blood peptidome allows to distinguish between cancer and non‐cancer samples, giving promise for a new MS‐based diagnostic tool. However, several aspects already published work have been criticized demand more methodical approach has formulated. Motivated by this we undertook systematic study plasma serum using an integrated ESI‐LC‐MS‐based platform, equipped with data tools relative absolute peptide quantitation. We used high...
To examine the relative role of halogen bonding and hydrophobic interactions in inhibition human CK2alpha by 4,5,6,7-tetrabromobenzotriazole (TBBt), we have synthesized a series 5-substituted benzotriazoles (Bt) corresponding 4,6,7-tribromobenzotriazoles (Br3Bt) examined their to that TBBt. The various C(5) substituents differ size (H CH3), electronegativity (NH2 NO2), hydrophobicity (COOH Cl). Some were bond donors (Cl, Br), while others fluorine (F CF3). Most analogues Br3Bt (with...
The binding of four bromobenzotriazoles to the catalytic subunit human protein kinase CK2 was assessed by two complementary methods: Microscale Thermophoresis (MST) and Isothermal Titration Calorimetry (ITC). New algorithm proposed for global analysis MST pseudo-titration data enabled reliable determination affinities distinct sites, a relatively strong one with Kd order 100 nM substantially weaker (Kd > 1 μM). site determined same protein-ligand systems using ITC were in most cases...
Replication of human immunodeficiency virus 1 (HIV-1) involves conversion its single-stranded RNA genome to double-stranded DNA, which is integrated into the host. This catalyzed by reverse transcriptase (RT), possesses DNA polymerase and RNase H domains. The available crystal structures suggest that at any given time RNA/DNA substrate interacts with only one active site two domains HIV-1 RT. Unknown whether a simultaneous interaction sites possible. Therefore, mechanism coordination...
The DXO family of proteins participates in eukaryotic mRNA 5'-end quality control, removal non-canonical NAD+ cap and maturation fungal rRNA precursors. In this work, we characterize the Arabidopsis thaliana homolog, DXO1. We demonstrate that plant-specific modification within active site negatively affects capping surveillance properties DXO1, but has only a minor impact on its strong deNADding activity. Unexpectedly, catalytic activity does not contribute to striking morphological...
ABSTRACT The list of mitochondrial DNA (mtDNA) variants detected in individuals with neurodegenerative diseases is constantly growing. Evaluating their functional consequences and pathogenicity not easy, especially when they are found only a limited number patients together wild-type mtDNA (heteroplasmy). Owing to its amenability genetic transformation incapacity stably maintain heteroplasmy, the strong evolutionary conservation proteins encoded mitochondria, Saccharomyces cerevisiae...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPulse radiolysis studies of intramolecular electron transfer in model peptides and proteins. 5. Trp.bul. .fwdarw. Tyr.bul. radical transformation H-Trp-(Pro)n-Tyr-OH series peptidesKrzysztof Bobrowski, Jerzy Holcman, Jaroslaw Poznanski, Marek Ciurak, Kazimierz L. WierzchowskiCite this: J. Phys. Chem. 1992, 96, 24, 10036–10043Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November...
Efficient repair by Escherichia coli AlkB dioxygenase of exocyclic DNA adducts 3,N(4)-ethenocytosine, 1,N(6)-ethenoadenine, 3,N(4)-α-hydroxyethanocytosine, and reported here for the first time 3,N(4)-α-hydroxypropanocytosine requires higher Fe(II) concentration than reference 3-methylcytosine. The pH optimum follows order pK(a) values protonation adduct, suggesting that positively charged substrates favorably interact with negatively carboxylic group Asp-135 side chain in enzyme active...
In ongoing studies on the role of individual bromine atoms 4,5,6,7-tetrabromobenzotriazole (TBBt) in its relatively selective inhibition protein kinase CK2α, we have prepared all possible two mono-, four di-, and tri-bromobenzotriazoles determined their physicochemical properties aqueous medium. They exhibited a general trend decrease solubility with an increase number bromines benzene ring, significantly modulated by pattern substitution. For given attached bromines, this was directly...