Dheer Singh

ORCID: 0000-0003-2686-0730
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About
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Research Areas
  • Reproductive Physiology in Livestock
  • Genetic and phenotypic traits in livestock
  • Reproductive Biology and Fertility
  • Animal Genetics and Reproduction
  • Cancer-related molecular mechanisms research
  • MicroRNA in disease regulation
  • Agricultural Science and Fertilization
  • Reproductive System and Pregnancy
  • Rice Cultivation and Yield Improvement
  • Extracellular vesicles in disease
  • Effects of Environmental Stressors on Livestock
  • RNA Interference and Gene Delivery
  • Crop Yield and Soil Fertility
  • Peroxisome Proliferator-Activated Receptors
  • Gut microbiota and health
  • Weed Control and Herbicide Applications
  • Ruminant Nutrition and Digestive Physiology
  • Molecular Biology Techniques and Applications
  • Agricultural Systems and Practices
  • Agronomic Practices and Intercropping Systems
  • Biosensors and Analytical Detection
  • Milk Quality and Mastitis in Dairy Cows
  • Cardiac, Anesthesia and Surgical Outcomes
  • Sugarcane Cultivation and Processing
  • Epigenetics and DNA Methylation

National Dairy Research Institute
2016-2025

Indian Oil Corporation (India)
2009-2023

Central Institute for Research on Buffaloes
2015-2023

Genomics (United Kingdom)
2020-2022

Guru Angad Dev Veterinary and Animal Sciences University
2021

Institut de Virologie
2021

Adesh University
2020-2021

Indian Council of Agricultural Research
2021

Maharana Pratap University of Agriculture and Technology
2020

Chhatrapati Shahu Ji Maharaj University
2019

IGF1, a potent stimulator of cellular proliferation, differentiation and development, regulates granulosa cell steroidogenesis apoptosis during follicular development. Depending upon species stage growth, IGF1 acts on either alone or together with FSH. We examined the mechanism action in bovine cells serum-free culture without insulin to determine its potential role regulation steroidogenic apoptotic regulatory gene expression investigate interaction FSH this mechanism. Bovine treated...

10.1530/rep-09-0050 article EN Reproduction 2009-10-10

Milk is not only a composite of nutrients but emerged as source exosomes acting promising drug delivery vehicle for small interfering RNA (siRNA). siRNA known its immense therapeutic potential has various physiological limitations, including stable delivery. To investigate the suitability stability and oral delivery, we encapsulated scrambled Alexa Fluor (AF)-488 in milk whey using lipofection evaluated against digestive processes along with uptake transepithelial transport by intestinal...

10.1021/acs.jafc.7b03123 article EN Journal of Agricultural and Food Chemistry 2017-10-02

Abstract Livestock, having close resemblance to humans, could be a better source of primary hepatocytes than rodents. Herein, we successfully developed three-dimensional (3D) culturing system for sheep and buffalo hepatocytes. The 3D-structures were formed on the fifth-day maintained until tenth-day polyHEMA-coated plates in hanging drops with William’s E media (HDW). Between cultured fresh cells, observed similar expression GAPDH, HNF4α, ALB, CYP1A1, CK8 CK18 . Interestingly, statistically...

10.1038/s41598-017-01355-6 article EN cc-by Scientific Reports 2017-04-20

ABSTRACT The variety CoP 92226 was selected for preparing sugarcane juice beverage on the basis of yield and sensory attributes from eight promising varieties sugarcane. Sugarcane samples were prepared by pasteurizing at 70°C 10 minutes adding citric acid (40 mg/100 ml), ascorbic ml) potassium metabisulphite (150 ppm). Samples stored room (30±5°C) refrigeration (4±2°C) temperature in pre-sterilized glass bottles analyzed physico-chemical, microbiological every 15 days interval 90 days. pH,...

10.1081/jfp-120015603 article EN International Journal of Food Properties 2002-10-15

Abstract Curcumin possesses anti‐inflammatory properties and provides a promising treatment for inflammation. The aim of the study is to establish that buffalo granulosa cells when primed with curcumin (20 μM), release improved cellular contents through exosome can mitigate cell dysfunction. Recently, we have shown exposed LPS (1 μg/mL) in serum free culture, transiently increased pro‐inflammatory cytokine genes (IL‐1β, TNF‐α, IL‐6) expression followed by inhibition CYP19A1 gene estradiol...

10.1002/jcb.26309 article EN Journal of Cellular Biochemistry 2017-08-03

Bovine granulosa cell culture models are important to understand molecular mechanisms of ovarian function. Folliculogenesis and luteinization associated with increasing density cells local hypoxic conditions. The current study identified two reliable housekeeping genes useful for gene normalization in under different vitro During the experiments were subjected biological physical stimuli, follicle stimulating hormone, initial plating hypoxia. Transcript abundance seven was quantified by...

10.1186/1477-7827-12-118 article EN cc-by Reproductive Biology and Endocrinology 2014-11-27

A facile, one‐step and greener route for the synthesis of gold nanoparticles (AuNPs) was developed in pursuit an eco‐friendly alternative via green nano‐materials. Mangifera indica (mango) is one most popular fruits key herb Ayurvedic ethno‐veterinary‐medical systems several thousand years. We report here first time AuNPs from auric chloride salt using aqueous extract M. flowers as reducing stabilizing agent. The biosynthesis process fast with forming within 20 minutes reaction‐kinetics...

10.1002/ep.12669 article EN Environmental Progress & Sustainable Energy 2017-06-26
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