A5102852605- Genomics, phytochemicals, and oxidative stress
- Garlic and Onion Studies
- Metalloenzymes and iron-sulfur proteins
- Metal-Catalyzed Oxygenation Mechanisms
- Metal Extraction and Bioleaching
- Amino Acid Enzymes and Metabolism
- X-ray Diffraction in Crystallography
- Zeolite Catalysis and Synthesis
- Analytical Chemistry and Chromatography
- High-pressure geophysics and materials
- Phytochemical compounds biological activities
- Photosynthetic Processes and Mechanisms
- Luminescence Properties of Advanced Materials
- Crystallization and Solubility Studies
- Electron Spin Resonance Studies
- Plant Disease Resistance and Genetics
- Chemical Reaction Mechanisms
- Glass properties and applications
- Microbial Community Ecology and Physiology
The University of Tokyo
2017-2020
Columbia University
1989
Planetary Science Institute
1980
University of California, Los Angeles
1980
Lachrymatory factor synthase (LFS) is responsible for the natural production of syn-propanethial S-oxide. Because its substrate and product are both transient, mechanistic insight currently poor. Here, we decipher catalytic cycle onion LFS (AcLFS) by employing crystallography, biochemical assays, molecular simulations. Crystal structures complexed with solute compounds demonstrate not only properties site but also a potent binding mode labile substrate, which can fulfill "syn-effect"...
Sulfur oxygenase reductases (SORs) are present in thermophilic and mesophilic archaea bacteria, catalyze oxygen-dependent oxygenation disproportionation of elemental sulfur. SOR has a hollow, spherical homo-24-mer structure reactions take place at active sites inside the chamber. The crystal structures SORs from Acidianus species have been reported. However, states site components (mononuclear iron cysteines) entry exit paths substrate products still dispute. Here, we report biochemical...
Abstract Natural lachrymatory effects are invoked by small volatile S -oxide compounds. They produced through alkene sulfenic acids the action of factor synthase (LFS). Here we present crystal structures onion LFS ( Ac LFS) revealed in solute-free and two solute-stabilized forms. Each structure adopts a single seven-stranded helix-grip fold possessing an internal pocket. Mutagenesis analysis localized active site to layer near bottom pocket, which is adjacent deduced key residues Arg71,...
Abstract Sulfur oxygenase reductases (SORs) are present in thermophilic and mesophilic archaea bacteria, catalyze oxygen-dependent oxygenation disproportionation of elemental sulfur. SOR has a hollow, spherical homo-24-mer structure reactions take place at active sites inside the chamber. The crystal structures SORs from two Acidianus species have been reported. However, states site components (mononuclear iron cysteines) entry exit paths substrate products still dispute. Here, we report...