A5043366358- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Clostridium difficile and Clostridium perfringens research
- Nosocomial Infections in ICU
- Antifungal resistance and susceptibility
- Mycobacterium research and diagnosis
- Fungal Infections and Studies
- Bacterial Infections and Vaccines
- Infectious Diseases and Tuberculosis
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Viral Infections and Immunology Research
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Parasites and Host Interactions
- Diagnosis and treatment of tuberculosis
- Cytomegalovirus and herpesvirus research
- Infective Endocarditis Diagnosis and Management
- Helminth infection and control
- Salmonella and Campylobacter epidemiology
University of British Columbia
2014-2024
B.C. Women's Hospital & Health Centre
2020-2024
Royal Pavilion
2023
British Columbia Children's Hospital
2010-2022
Children's & Women's Health Centre of British Columbia
2008-2019
BC Centre for Disease Control
2013
Child and Family Research Institute
2009
Vancouver General Hospital
2008
Sultan Qaboos University
2008
We assessed the performance, stability, and user acceptability of swab-independent self-collected saliva saline mouth rinse/gargle sample types for molecular detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in adults school-aged children. Outpatients who had recently been diagnosed with COVID-19 or were presenting suspected asked to have a nasopharyngeal (NP) swab collected provide at least one type.
This article reports a case of 21-year-old woman with refractory B-cell acute lymphocytic leukaemia who presented severe respiratory syndrome coronavirus-2 (SARS-CoV-2). She remained positive for SARS-CoV-2 by viral culture 78 days and polymerase chain reaction (PCR) 97 days. Sequencing repeat samples over time demonstrated an increasing dynamic repertoire mutations.
Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of multi-drug-resistant infections in people, particularly indigent populations. MRSA can be transmitted between people and domestic animals, but the potential for transmission commensal pests, rodents, had not been investigated. The objective this study was to identify presence characterize ecology rats (Rattus spp.) from impoverished, inner-city neighborhood. Oropharyngeal swabs were collected trapped 33 city blocks...
We investigated epidemiologic and molecular characteristics of healthcare-associated (HA) community-associated (CA) Clostridioides difficile infection (CDI) among adult patients in Canadian Nosocomial Infection Surveillance Program hospitals during 2015-2019. The study encompassed 18,455 CDI cases, 13,735 (74.4%) HA 4,720 (25.6%) CA. During 2015-2019, rates decreased by 23.8%, whereas CA 18.8%. was significantly associated with increased 30-day all-cause mortality as compared (p<0.01). Of...
A survey in 2000 to detect methicillin-resistant Staphylococcus aureus (MRSA) colonization Vancouver downtown east side injection drug users (IDUs) revealed an MRSA nasal incidence of 7.4%. This is a follow-up study determine the current prevalence and further characterize isolates risk factors for colonization. In this point carriage among IDUs, swabs were cultured S. aureus. Isolates studied their antimicrobial susceptibility patterns presence mecA Panton-Valentine leukocidin (PVL) genes...
OBJECTIVE: To review the epidemiology and associated risk factors for candidemia at a tertiary care centre, in view of recent reports on changing bloodstream infection due to Candida species. METHODS: Between January 2000 December 2009, patients with blood culture samples positive species were identified using microbiology laboratory information system. Patient data collected by retrospective chart clinical characteristics including demographic data, underlying medical diagnoses factors....
Abstract Background We assessed the performance, stability, and user acceptability of swab-independent self-collected saliva saline mouth rinse/gargle sample types for molecular detection SARS-CoV-2 in adults school-aged children. Methods Outpatients who had recently been diagnosed with COVID-19 or were presenting suspected asked to have a nasopharyngeal swab collected provide at least one type. A portion participants also about acceptability. Samples underwent testing using multiple assays....
The coronavirus disease 2019 (COVID-19) pandemic has placed significant burden on healthcare systems. We compared Clostridioides difficile infection (CDI) epidemiology before and during the across 71 hospitals participating in Canadian Nosocomial Infection Surveillance Program. Using an interrupted time series analysis, we showed that CDI rates significantly increased COVID-19 pandemic.
Candidemia represents a significant cause of morbidity and mortality in children. We examined the epidemiology associated risk factors candidemia at Canadian tertiary care paediatric hospital over an 11-year period.A retrospective chart review was conducted on children with positive blood culture for Candida species between January 1, 2007 December 31, 2018. Patient demographics, previously described factors, species, follow-up investigations, interventions, outcome data were included...
The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and -80°C was assessed by real-time PCR. In purified nucleic acid extracts, both were stable for up to 30 days, irrespective temperature. unextracted samples, temperature-dependent loss (P < 0.05) observed serum cerebrospinal fluid specimens, while no changes EDTA blood samples.
Abstract Clostridium difficile is the leading cause of healthcare‐associated infections worldwide. The diagnosis C. infection (CDI) in pediatric oncology patients complex as diarrhea common, and there a high rate colonization infants young children. This study was conducted to assess accuracy surveillance definitions CDI (HA‐CDI) determine prevalence toxigenic among stem cell transplant patients. Methods A prospective cohort over three‐year period an inpatient setting. Baseline stool samples...
The genus Aurantimonas, proposed in 2003, encompasses four species from environmental sources, including Aurantimonas altamirensis, isolated a cave wall Spain. Here, we report what believe are the first cases of recovery A. altamirensis human clinical materials.
To identify the prevalence of C. auris in Canadian patients who are potentially at risk for colonization, we screened 488 were either hospitalized abroad, had a carbapenemase-producing organism (CPO), or units with high antifungal use. Two colonized auris; both received healthcare India and CPO. Among 35 recently Indian subcontinent CPO infected, was 5.7%.
A man with Escherichia coli meningitis and bacteremia, while on dexamethasone, developed Strongyloides hyperinfection syndrome died despite salvage therapy subcutaneous ivermectin. We report the first documented total free levels of ivermectin used for therapy.
Brain abscess, while rare, confers high mortality, especially in the developing world. The case of a Ugandan child with polymicrobial brain abscess including infection Tissierella praeacuta/Clostridium hastiforme requiring repeated drainage and eventual surgical excision is reported. demonstrates importance considering anaerobic organisms treatment children from
BACKGROUND: Bordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays detect B are typically based on the multicopy insertion sequence IS 481 , which offers high sensitivity but lacks species specificity. METHODS: A novel real‐time PCR assay porin gene was tested parallel with several previously published that target genes such as ptx ‐promoter, pertactin and putative thialase. The were evaluated using reference panel of...
As part of the COVID-19 pandemic, clinical laboratories have been faced with massive increases in testing, resulting sample collection systems, reagent, and staff shortages. We utilized self-collected saline gargle samples to optimize high throughput SARS-CoV-2 multiplex polymerase chain reaction (PCR) testing order minimize cost technologist time. This was achieved through elimination nucleic acid extraction automation handling on a widely available robotic liquid handler, Hamilton STARlet....
Clostridium difficile is the most common cause of antibiotic-associated diarrhea, and it occasionally causes extraintestinal infections. We present a case C. difficile–associated diarrhea that led to vertebral osteomyelitis associated with hardware. The became symptomatic 2 years after initial diarrheal event. recovered from internal hardware sites cannot simply be regarded as contaminant but should treated.