A5035871532- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
- Bone Tissue Engineering Materials
- Cardiomyopathy and Myosin Studies
- Pluripotent Stem Cells Research
- Cardiac Structural Anomalies and Repair
- Angiogenesis and VEGF in Cancer
- Cardiovascular Function and Risk Factors
- 3D Printing in Biomedical Research
- Cardiac pacing and defibrillation studies
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- biodegradable polymer synthesis and properties
Imperial College London
2018-2024
University of Helsinki
2021-2024
Medium-chain length polyhydroxyalkanoates (MCL-PHAs) have demonstrated exceptional properties for cardiac tissue engineering (CTE) applications. Despite prior work on MCL-PHA/polycaprolactone (PCL) blends, optimal scaffold production and use as an alternative delivery route controlled release of seeded progenitor cells (CPCs) in CTE applications vivo has been lacking. We present herein applicability MCL-PHA/PCL (95/5 wt %) blends fabricated thin films with improved performance compared to...
Organ dysfunction is a major cause of morbidity and mortality. Transplantation typically the only definitive cure, challenged by lack sufficient donor organs. Tissue engineering encompasses development biomaterial scaffolds to support cell attachment, proliferation, differentiation, leading tissue regeneration. For efficient clinical translation, forming technology utilized must be suitable for mass production. Herein, uniaxial polyhydroxyalkanoate manufactured pressurized gyration, hybrid...
Coronary microvascular disease (CMD) and its progression towards major adverse coronary events pose a significant health challenge. Accurate in vitro investigation of CMD requires robust cell model that faithfully represents the cells within cardiac microvasculature. Human pluripotent stem cell-derived endothelial (hPSC-ECs) offer great potential; however, they are traditionally derived via differentiation protocols not readily scalable specified Here, we report development comprehensive...
Background: Left ventricular hypertrabeculation (LVHT) is a heterogenous cardiac condition with complex and poorly understood aetiology. We comprehensively characterised the effect of novel P1891A mutation in SCN5A gene, which encodes voltage-gated sodium channel Nav1.5, identified Finnish family diagnosed LVHT. Methods: generated SCN5A-P1891A mutation-carrying human induced pluripotent stem cell-derived cardiomyocytes (P1891A-hiPSC-CMs) performed electrophysiological assessments, including...
<title>Abstract</title> Cardiac microvascular disease (CMD) and its progression towards major adverse coronary events pose a significant health challenge. Accurate in vitro investigation of CMD requires robust cell model that faithfully represents the cells within cardiac microvasculature. Human pluripotent stem cell-derived endothelial (hPSC-ECs) offer great potential; however, they are traditionally derived via differentiation protocols not readily scalable specified Here, we report...
<title>Abstract</title> Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold promise in averting functional decline towards heart failure following myocardial infarction (MI). However, challenges persist retaining these cells within the myocardium. We developed a novel three-dimensional cardiac patch and evaluated its potential to prevent post-MI decline. For first time, melt electrowritten (MEW), structurally anisotropic scaffolds were derived from high molecular weight,...
Abstract Introduction Left ventricular noncompaction (LVNC) is a heterogenous cardiomyopathy characterised by an extensively trabeculated left ventricle (LV). Several genetic variants that perturb trabeculae compaction, and thus LV maturation, have been reported [1]. However, the precise mechanisms underpinning this proven contentious with, for example, differentially aberrant cardiomyocyte proliferation implicated in development of LVNC [2,3]. Purpose In study, we investigate novel SCN5A...
Introduction: Coronary microvascular disease (CMD) can manifest as angina or HFpEF and is implicated in post-STEMI remodelling COVID-19. Superior vitro models would aid the development of therapies for CMD. This study outlines creation a 3D differentiation protocol that yields stable human pluripotent stem cell-derived microvascular-like endothelial cells (hPSC-MVECs). Hypothesis: We hypothesised organoid environment allow recapitulation microvasculature. Methods: Growth factor-mediated...
Abstract Introduction The microvasculature is a vital constituent of the adult human vasculature 1. Cardiac tissue engineering, in vitro models cardiac remodelling and regeneration, disease would benefit from inclusion well-characterised, phenotypically stable microvascular-like endothelial cells (ECs) that could be generated at scale without xenogeneic reagents. Primary microvascular ECs have been utilised for such applications however, they are susceptible to passaging-induced senescence²....